DH10B 感受态细胞

Product box and its contents: 5 tubes of ElectroMAX DH10B Cells, 1 vial of pUC19 DNA, and 2 bottles of SOC medium

什么是 DH10B 感受态细胞？

大肠杆菌 DH10B 菌株 由于其具有高转化效率，可摄取和容纳大质粒 (>30 kb) ，缺乏甲基化依赖性限制性系统 (MDRS) ，以及可进行蓝白斑菌落筛选，成为日常分子克隆应用的常用菌株。DH10B α 可摄入基因组 DNA ，是其与 DH5 α 菌株的主要区别。完整的 DH10B 基因组已被 Durfeet 等人测序并于 2008 年发布 [1]。

DH10B 感受态细胞的优势

基因组突变使其成为 cDNA 和基因组 DNA 文库转化的理想选择
蓝白斑筛选，便于重组 DNA 菌落鉴定
有化学或电转感受态可供选择

哪种DH10B感受态细胞适合您?

	MAX Efficiency DH10B 感受态细胞	MAX Efficiency DH10B T1 噬菌体抗性感受态细胞*	ElectroMAX DH10B 细胞	ElectroMAX DH10B T1 噬菌体抗性感受态细胞	MegaXDH10B T1R 电转感受态细胞

适用于	应用广泛的高效感受态细胞	应用广泛方便的单次使用或高通量形式	高转化效率文库构建和其他具有挑战性的实验应用	高转化效率文库构建和其他具有挑战性的实验应用	更高的转化效率高要求的分子克隆文库构建
转化效率	>1 x 10⁹ cfu/µg	>1 x 10⁹ cfu/µg	>1 x 10¹⁰ cfu/µg	>1 x 10¹⁰ cfu/µg	>3 x 10¹⁰ cfu/µg
转化类型	化学修饰	化学修饰	电穿孔	电穿孔	电穿孔
抗噬菌体 T1 、 T5 和 φ80 γ 污染	–	✓	––	✓	✓
可提供的规格	5 x 200 µL/tube	21 x 50 µL/tube 1 x 96 well plate with 20 µL/well	5 x 100 µL/tube	5 x 100 µL/tube	5 x 100 µL/tube

* 菌株也可以高通量的 FlexPlate 形式提供。

参考文献

Durfee R, Nelson R, Baldwin S, et al (2008) The Complete Genome Sequence of Escherichia coli DH10B: Insights into the Biology of a Laboratory Workhorse. J Bacteriol 190(7):2597–2606.

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