Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
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| 2 | 405 | 810/25 | 408 | 800 | (in buffer) 3 | flow cytometry |
Invitrogen NovaFluor Violet 800 dye has a unique emission spectrum when excited by the 404 nm laser, which fits in a spectral space that is currently not accessible by other spectral dyes. Adding spectrally unique dyes may allow for building out larger panels with minimal loss of resolution. NovaFluor Violet 800 dye should generally be paired with moderately to highly expressed antigens in multicolor panels. The macromolecule-based NovaFluor Violet 800 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.
Additional products
- Narrow emission spectrum and minimal cross-laser excitation for better resolution
- Should be paired with moderately expressed antigens to minimize spillover
- Spectrally unique and can be used to fill unused detectors in conventional flow cytometry
- Produces stable fluorescence and is compatible with all buffers tested
Click image to enlarge
Spectral signature of NovaFluor Violet 800 dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Violet 800 dye were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
Click image to enlarge
NovaFluor Violet 800 dye is compatible with spectrally adjacent fluorophores including BV711, BV786, NovaFluor Violet 690 and NovaFluor Violet 745 dyes. Human peripheral blood cells (PBMCs) were stained with CD3 Monoclonal Antibody, Brilliant Violet™ 711 (left), CD4 NovaFluor Violet 745 dye, CD8a Monoclonal Antibody, Brilliant Violet™ 786 (middle), CD45RA NovaFluor Violet 800 dye, and CD27 NovaFluor Violet 690 dye (right). The CD3-positive gate was used for analysis in the middle and right plots. Viable cells were used for analysis, as determined by LIVE/DEAD Fixable Blue Dead Cell Stain Kit, for UV excitation. Data was collected on a 5-laser Cytek Aurora Flow Cytometer.
NovaFluor dyes
NovaFluor dyes are built using Phiton technology and are compatible with both spectral flow cytometry and traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, which helps reduce spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.
Additional resources
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.