Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
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| 2 | 405 | 660/20, 630 LP | 407 | 646 | (in buffer) 3 | flow cytometry |
Invitrogen Brilliant Violet™ 650 (BV650) is a tandem dye excited by the 405 nm violet laser and emits at 650 nm. This dye has a low-to-medium relative brightness and can be used for cell surface, intracellular, and nuclear staining. BV650 may have some cross-laser excitation with the red laser.
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye- conjugated antibodies in a staining panel, we recommend using Super Bright Complete Staining Buffer or Brilliant Stain Buffer to minimize any non-specific polymer interactions.
We offer BV650 dye conjugated to primary antibodies for use in flow cytometry.
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Spectral signature of Brilliant Violet™ 650 dye. Data acquired on a 5-laser Cytek Aurora and normal human peripheral blood cells stained with CD8a Monoclonal Antibody (RPA-T8), Brilliant Violet™ 650 were used for analysis.
Intracellular staining of human peripheral blood cells using Brilliant Ultra Violet™ 650. Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with the Cell Stimulation Cocktail (plus protein transport inhibitors) (right). Cells were then stained intracellularly, using Intracellular Fixation & Permeabilization Buffer Set, CD4 Monoclonal Antibody (RPA-T4), FITC and TNF alpha Monoclonal Antibody, Brilliant Violet™ 650. Viable cells in the lymphocyte gate were used for analysis, as determined by Fixable Viability Dye eFluor™ 780.
Cell surface staining of mouse splenocytes using Brilliant Violet™ 650. C57BL/6 mouse splenocytes were stained with CD3e Monoclonal Antibody, FITC and 0.25 µg of Rat IgG2a kappa Isotype Control, Brilliant Violet™ 650 (left) or 0.25 µg of CD4 Monoclonal Antibody (RM4-5), Brilliant Violet™ 650 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD Viability Staining Solution.
Brilliant Ultra Violet™ dye background
Brilliant Ultra Violet dyes are a polymer dye-based technology and compatible with both spectral flow cytometry as well as traditional flow cytometry. The UV laser has unique channels far from heavily occupied detectors, allowing for larger panels.
Additional resources
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.
Brilliant Ultra Violet™ 和 Brilliant Violet™ 是 Becton, Dickinson 公司或其附属公司的商标或注册商标，经许可使用。
仅供科研使用，不可用于诊断目的。