Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
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| 5 | 430 | CFP | 427 | 499 | 5 (in antifade) | Microscopy |
Invitrogen Aluora dyes, including Aluora 430 dye, are a specialized organic dye technology specifically optimized for spatial biology and multiplex immunohistochemistry (IHC) applications. Aluora 430 dye is excited by the 430 nm laser and emits at 499 nm. This dye has high brightness and photostability. To learn more about spatial imaging, visit our Spatial Biology Resource Center.
We offer Aluora 430 Spatial Amplification reagents for use in immunohistochemistry.
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Human FFPE small intestine was incubated with 1:1,000 of Biotinylated Ki-67 Monoclonal Antibody (SolA15) (Cat. No. 13-5698-82) then labeled with the reagents in the Aluora 430 HRP-streptavidin Kit (Cat. No. A40001345) and a DAPI nuclear stain. The slides were mounted in ProLong Glass mountant. Images were captured with an Invitrogen EVOS M7000 instrument (see information about EVOS imaging systems).
Aluora dyes
Invitrogen Aluora dyes are a specialized organic dye technology specifically optimized for spatial biology and multiplex immunohistochemistry applications. These bright, photostable dyes are excellent for detecting extremely low-abundance targets. Additionally, Aluora dyes exhibit a distinct emission spectra fingerprint that can be spectrally unmixed for high plex imaging experiments on spatial imaging platforms such as the EVOS S1000 Spatial Imaging System.
Additional resources
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.
Brilliant Ultra Violet™ 和 Brilliant Violet™ 是 Becton, Dickinson 公司或其附属公司的商标或注册商标，经许可使用。
仅供科研使用，不可用于诊断目的。