Learn about different research areas where cell analysis plays an important role. Find application notes and articles, scientific posters, webinars and other relevant content on cancer, exosomes, neuroscience, stem cell research, immunology, and more.
The focus of the Schmitt-Ulms laboratory at the University of Toronto is the development of models and methods for studying proteins that cause neurodegenerative diseases, with the goal of generating insights that will lead to novel angles for diagnosis or intervention
Parkinson’s disease (PD) is a progressive neurodegenerative disorder that affects 1% of people over age 60 and more than 5 million people worldwide. PD results from the selective loss of dopaminergic neurons in the substantia nigra region of the brain. The loss of these neurons initially affects movement. As the disease progresses, however, cognitive function is impaired, and late-stage disease is often accompanied by dementia.
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| Type | Title | Categories |
|---|---|---|
| Application note (2011) | Detection of human mesenchymal stem cells using the Attune Acoustic Focusing Cytometer | Attune/Attune NxT, cell cycle, flow cytometer/flow cytometry, immunophenotyping, stem cell research |
| Application note (2012) | Monitoring neurite morphology and synapse formation in primary neurons for neurotoxicity assessments and drug screening | ArrayScan, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, neuroscience, viability |
| Application note (2013) | Optimizing the high content analysis of cell morphology using multi-dimensional imaging on the Thermo Scientific ArrayScan XTI HCA Reader: Stem cell colonies | Alexa Fluor, antibodies, ArrayScan, confocal microscopy, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, immunofluorescence (IF), microplate reader, particles, stem cell research |
| Application note (2014) | Using a high content analysis database as a repository for data | database management, drug discovery, high content analysis, microplate reader |
| Application note (2014) | High content cell cycle screening: Pairing FUCCI technology with Thermo Scientific HCS Studio 2.0 Software and FCS Express Image cytometry | ArrayScan, cancer, cell cycle, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader |
| Application note (2015) | No-wash, no-lyse detection of phagocytic cells via a pHrodo BioParticles functional assay in human whole blood on the Attune NxT Flow Cytometer | antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immune system, immunophenotyping, particles, pH detection |
| Application note (2015) | 13-parameter immunophenotyping of human lysed whole blood with the the Attune NxT Flow Cytometer allows for the identification of B cells, NK cells, multiple T cell subsets and myeloid cells | Alexa Fluor, antibodies, Attune/Attune NxT, cancer, flow cytometer/flow cytometry, fluorescent dyes, immune system, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot |
| Application note (2017) | Simple and accurate monitoring of adipogenesis | adipocyte, adipogenesis, automated cell counter, cell differentiation, Countess, EVOS, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, onstage incubator, stem cell research |
| Application note (2017) | Flow cytometry analysis of dose response for apoptosis induction | apoptosis, Attune/Attune NxT, cancer, flow cytometer/flow cytometry |
| Application note (2017) | Flow cytometry analysis of transcription factor expression during differentiation of hPSC-derived cardiomyocytes | antibodies, Attune/Attune NxT, cardiomyocytes, cell differentiation, flow cytometer/flow cytometry, fluorescence, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunophenotyping, stem cell research |
| BioProbes articles (Issues 50– present day) | BioProbes Journal of Cell Biology Application | cell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification |
| Molecular Probes Handbook | Mitochondria in diseases—Note 12.2 | antibodies, cell structure-mitochondria, fluorescence microscopy/fluorescence imaging |
| Molecular Probes Handbook | Sphingolipids, steroids, lipopolysaccharides and related probes—Section 13.3 | fluorescent dyes, lipid metabolism, lipid tracking |
| Scientific poster (2006) | LipidTOX dyes for adipocyte staining in routine imaging applications | cell differentiation, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, lipid detection, lipids, stem cell research, viability |
| Scientific poster (2008) | Quantum dot (Qdot) labeling of gene expression in fresh frozen brain tissue using high-throughput in situ hybridization | in situ hybridization, brightfield microscopy, confocal microscopy,fluorescence microscopy/fluorescence imaging, fluorescent dyes, gene expression, high content analysis, nanocrystals, neuroscience, Qdot, tissue |
| Scientific poster (2008) | FluxOR, a universal potassium channel assay: Screening BacMam-delivered and stably expressed targets | BacMam technology, drug discovery, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader |
| Scientific poster (2008) | Modified tumor cell protein expression and modification under physiological oxygen | Alexa Fluor, cancer, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, physiological oxygen level, protein labeling |
| Scientific poster (2008) | Targeting serum glycoprotein cancer biomarkers with click chemistry | cancer, Click-iT, Electrophoresis gel, fluorescent dyes, gel electrophoresis, protein labeling |
| Scientific poster (2009) | Dual pulse labeling of S-phase population using click chemistry to measure changes in cell proliferation | Alexa Fluor, cancer, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes |
| Scientific poster (2009) | Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry | Alexa Fluor, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, nucleic acid quantitation, stem cell research, violet laser-excited reagents |
| Scientific poster (2009) | A novel xeno-free and serum-free culture medium for the expansion of human mesenchymal stem cells | cell culture/expansion, flow cytometer/flow cytometry, fluorescent dyes, gene expression, stem cell research |
| Scientific poster (2009) | Metabolic labeling and click chemistry detection of stem cell markers | Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein labeling, stem cell research |
| Scientific poster (2009) | Cell-based potassium ion channel screening with BacMam using the FluxOR Assay | BacMam technology, drug discovery, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader |
| Scientific poster (2010) | Acoustic flow cytometry for multicolor immunophenotyping analysis | antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, immune system, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot, violet laser-excited reagents |
| Scientific poster (2010) | Analysis of proliferation dynamics in primary and immortalized human cells | Alexa Fluor, cancer, cell proliferation, CellTrace, circulatory system, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, fluorescent proteins, nucleic acid quantitation |
| Scientific poster (2010) | Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry and imaging | Alexa Fluor, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF), immunophenotyping, stem cell research, violet laser-excited reagents |
| Scientific poster (2010) | A new generation of cellular imaging probes for neurosciences research | autophagy, BacMam technology, CellLight, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels, ion indicators |
| Scientific poster (2010) | NA-Fluor Influenza Neuraminidase Assay Kit: Standardization of the classic MUNANA-based neuraminidase activity assay for monitoring antiviral resistance in influenza | drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, influenza, microbiology, microplate reader |
| Scientific poster (2010) | A comparison of three techniques to induce efficient ex vivo T cell expansion | Alexa Fluor, antibodies, cell proliferation, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, immune system, magnetic beads, multicolor flow cytometry, viability |
| Scientific poster (2010) | The NA-XTD Influenza Neuraminidase Assay Kit: Extended-glow chemiluminescence assay system for highly sensitive influenza neuraminidase assays | chemiluminescence, drug discovery,fluorescence microscopy/fluorescence imaging, high content analysis, influenza, microbiology, microplate reader |
| Scientific poster (2010) | Dual pulse labeling using EdU and BrdU to monitor alterations in cellular proliferation by fluorescence microscopy and flow cytometry | Alexa Fluor, cell cycle, cell proliferation, Click-iT, drug discovery, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Scientific poster (2011) | Characterization of human limbal stem cells using acoustic focusing cytometry | Attune/Attune NxT, cell culture/expansion, flow cytometer/flow cytometry, fluorescent dyes, rare-event detection, stem cell research, tissue, violet laser-excited reagents |
| Scientific poster (2011) | Acoustic cytometric identification and initial characterization of limbal stem cells isolated from human cornea | Attune/Attune NxT, cell culture/expansion, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, PCR/qPCR, stem cell research, tissue, violet laser-excited reagents |
| Scientific poster (2011) | Primary human cells from Life Technologies: Offerings, scale and applications in HCS and HTS formats | Alexa Fluor, antibodies, autophagy, BacMam technology, cell proliferation, cell signaling, Click-iT, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis |
| Scientific poster (2011) | NA-Fluor and NA-XTD Influenza Neuraminidase Assays for neuraminidase quantitation and inhibition assays | chemiluminescence, drug discovery,fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, influenza, microbiology, microplate reader |
| Scientific poster (2012) | Rapid, in vitro T lymphocyte cell tracing by acoustic focusing cytometry | Attune/Attune NxT, cell proliferation, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, magnetic beads, stem cell research |
| Scientific poster (2012) | Characterization of human limbal stem cells | Attune/Attune NxT, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, rare-event detection, stem cell research, tissue, violet laser-excited reagents |
| Scientific poster (2014) | Flow cytometry multiplexing used to analyze metabolic activity and assess pharmaceutical compound toxicity as a high-throughput screening tool | Attune/Attune NxT, drug discovery, flow cytometer/flow cytometry |
| Scientific poster (2016) | High content screening in MCF7 and MDA-MB231 cells show differential responses depending on oxygen levels and mechanistic readout for viability | cell health, cell viability and function, drug discovery, high content analysis, high throughput screening |
| Scientific poster (2016) | Novel in vitro method for screening inhibitors of protein translation | cancer, drug discovery, high throughput screening, microplate reader, protein translation |
| Scientific poster (2018) | A Laser Based High Content Analysis Platform Improves Speed, Sensitivity and Axial Resolution for Quantitative Imaging | High-Content Analysis, High-Content Screening, Quantitative Imaging, Phenotypic Screening, Drug Discovery, 3D Cell Cultures, Spheroids, CX7 LZR |
| Scientific poster (2018) | Analysis Of Cellular Processes Within 3D Cell Models Using Fluorescence Microscopy | High-Content Analysis, High-Content Screening, Quantitative Imaging, Phenotypic Screening, Drug Discovery, 3D Cell Cultures, Spheroids, CX7 LZR |
| Webinar | Breathing new life into hypoxia research Although the significance of hypoxia in biological processes is well known, creating model systems with accurate control of hypoxic conditions is extremely difficult without access to elaborate systems that allow precise control and maintenance of temperature, humidity, and gases (CO2 and O2) during an experiment. In this webinar, we will discuss: Overview of hypoxia in human diseases Classical methods for setting up hypoxic conditions Novel instruments and reagents for imaging cells in hypoxic conditions | EVOS, fluorescence microscopy/fluorescence imaging, high content analysis, hypoxia, onstage incubator |
不可转售。Super Bright聚合物染料由Becton, Dickinson and Company授权销售。
仅供科研使用，不可用于诊断目的。
