Table 6.8 Summary of biotin-, streptavidin- and NeutrAvidin biotin-binding protein–labeled FluoSpheres microspheres.
Microspheres (Ex/Em) * |
0.04 µm
|
0.2 µm
|
1.0 µm
|
---|---|---|---|
Biotin-Labeled Microspheres | |||
Yellow-green (505/515) | F8766 0.4 mL | F8767 0.4 mL | F8768 0.4 mL |
Nonfluorescent | F8769 0.4 mL | ||
Streptavidin-Labeled Microspheres | |||
Yellow-green (505/515) | F8780 0.4 mL | ||
NeutrAvidin-Labeled Microspheres | |||
Europium luminescent (340–370/610) | F20883 0.4 mL | F20884 0.4 mL | |
Yellow-green (505/515) | F8771 0.4 mL | F8774 0.4 mL | F8776 0.4 mL |
Red (580/605) | F8770 0.4 mL | F8775 0.4 mL | |
Nonfluorescent | F8772 0.4 mL | F8777 0.4 mL | |
* Excitation (Ex) and emission (Em) maxima in nm. The streptavidin-labeled FluoSpheres beads are supplied as aqueous suspensions containing 0.5% solids and 5 mM sodium azide. The NeutrAvidin biotin-binding protein–labeled europium luminescent FluoSpheres are supplied as aqueous suspensions containing 0.5% solids and 5 mM azide. Other NeutrAvidin biotin-binding protein– and biotin-labeled FluoSpheres beads are supplied as aqueous suspensions containing 1% solids, 5 mM sodium azide and 0.02% Tween. All sizes fall within a narrow range as discussed in Microspheres—Section 6.5. Sizes indicated in the above tables are nominal and may vary from batch to batch. Actual sizes, as determined by electron microscopy, are specified on the product label. |
For Research Use Only. Not for use in diagnostic procedures.