Amine-Reactive, Ultraviolet Light–Excitable Fluorophores for Labeling Proteins and Nucleic Acids—Table 1.12

• Higher fluorescence per attached dye than AMCA
• Protein conjugates emit at slightly shorter wavelengths than AMCA or AMCA-X

• Cascade Blue derivative containing a spacer between the fluorophore and the reactive SE
• Near-perfect match to the 405 nm violet diode laser

• Extremely large Stokes shift
• Fills spectral gap between green- and yellow-fluorescent dyes

• Widely used blue-fluorescent labeling dye
• Compact structure

• Blue-fluorescent dye
• Small size

• Resistant to quenching upon protein conjugation
• Trisulfonated pyrene

• Longer-wavelength alternatives to AMCA

• pH-sensitive fluorescence
• Compact structure

• Optimal for 365 nm excitation sources

• pH-insensitive fluorescence
• Compact structure

• Longer-wavelength alternative to the Alexa Fluor 350 and AMCA-X dyes
• Excited with the 405 nm violet diode laser

• Excited with the 405 nm violet diode laser
• Compatible with the Pacific Blue dye for two-color analysis using the blue diode laser

* The absorption (Abs) and fluorescence emission (Em) maxima, in nm, listed in this table are for the goat anti–mouse IgG antibody or dextran conjugates in aqueous buffer.

(1) Spectral maxima for D374.
(2) Spectral maxima for D1412.
(3) Spectral maxima for H1193.
(4) Spectral maxima for 7-hydroxy-4-methylcoumarin-3-acetic acid, succinimidyl ester.

(AA) = Acetyl azide.
(Ab) = Antibody Labeling Kit (Kits for Labeling Proteins and Nucleic Acids—Section 1.2).
(APEX) = APEX Antibody Labeling Kit (Kits for Labeling Proteins and Nucleic Acids—Section 1.2).
(COOH) = Carboxylic acid.
(ITC) = Isothiocyanate.
(P) = Easy-to-Use Protein Labeling Kit (Kits for Labeling Proteins and Nucleic Acids—Section 1.2).
(SE) = Succinimidyl ester.
(Z) = Zenon Antibody Labeling Kit (Zenon Technology: Versatile Reagents for Immunolabeling—Section 7.3).