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Table 7.10 Molecular Probes lectin conjugates.
Label (Abs/Em) * |
Lectin Conjugate †
| |||||||
---|---|---|---|---|---|---|---|---|
Con A | WGA | IB4 | GS-II | PHA-L | HPA | SBA | PNA | |
Alexa Fluor 350 (346/442) | C11254 | W11263 | ||||||
Fluorescein (494/518) | C827 | W834 | ||||||
Alexa Fluor 488 (495/519) | C11252 ‡ | W11261 | I21411 | L21415 | L11270 | L11271 | L11272 | L21409 |
Oregon Green 488 (496/524) | W6748 | |||||||
Alexa Fluor 555 (555/565) | W32464 | |||||||
Tetramethylrhodamine (555/580) | C860 | W849 | ||||||
Alexa Fluor 568 (578/603) | I21412 | L32458 | ||||||
Alexa Fluor 594 (590/617) | C11253 | W11262 | I21413 | L21416 | L32456 | L32462 | L32459 | |
Texas Red (595/615) | C825 | |||||||
Texas Red-X (595/615) | W21405 | |||||||
Alexa Fluor 633 (632/647) | C21402 | W21404 | ||||||
Alexa Fluor 647 (650/668) | C21421 | W32466 | I32450 | L32451 | L32457 | L32454 | L32463 | L32460 |
Alexa Fluor 680 (679/702) | W32465 | |||||||
Qdot 655 (405/655) § | Q12021MP | |||||||
Biotin-XX (NA) | I21414 | |||||||
* Approximate absorption (Abs) and fluorescence emission (Em) maxima, in nm, for conjugates. † Lectin sources: Concanavalin A (Con A) isolated from Canavalia ensiformis (jack bean); Wheat Germ Agglutinin (WGA) isolated from Triticum vulgaris; IB4 isolated from Griffonia simplicifolia (an African legume); GS-II isolated from Griffonia simplicifolia; PHA-L isolated from Phaseolus vulgaris (red kidney bean); HPA isolated from Helix pomatia (edible snail); SBA isolated from Glycine max (soybean); PNA isolated from Arachis hypogaea (peanut). ‡ Succinylated Alexa Fluor 488 concanavalin A is also available, C21401. § The properties and applications of Qdot nanocrystal conjugates are described in Qdot Nanocrystals—Section 6.6. NA = Not applicable. For researchers interested in testing our fluorescent WGA conjugates in their application, we also offer a Wheat Germ Agglutinin Stain Sampler Kit (W7024), which contains 1 mg quantities each of WGA conjugates of the Alexa Fluor 350, Oregon Green 488, tetramethylrhodamine and Texas Red-X dyes. |
For Research Use Only. Not for use in diagnostic procedures.