Polymer-based columns for carbohydrate analysis
Need a column to separate carbohydrates using dilute acid mobile phase? These polymeric ligand exchange columns utilize a range of counter ions to deliver selectivity required for the analysis of carbohydrates, organic acids, and sugar alcohols. Thermo ScientificHyperREZ XP HPLC columns are available in H+, Ca+2, Pb+2, and Na+ forms.
Column | Particle Size | Porosity | Applications |
|---|---|---|---|
| HyperREZ XP Carbohydrate Ca2+ LC Columns | 8μm | 8% cross linkage |
|
| HyperREZ XP Carbohydrate Pb2+ LC Columns | 8μm | 8% cross linkage |
|
| HyperREZ XP Carbohydrate H+ LC Columns | 8μm | 8% cross linkage |
|
| HyperREZ XP Carbohydrate Na+ LC Columns | 10μm | 4% cross linkage |
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HyperREZ XP Carbohydrate columns are based on a monodisperse resin with 4 or 8% divinylbenzene content, and provide an ideal medium for the analysis of carbohydrates and organic acids. Unlike silica based columns they are stable at low pH, allowing the use of dilute acid as a mobile phase. The columns can also be run at elevated temperatures for faster analysis and improved resolution of some closely eluting analytes. The columns can be easily regenerated for increased column lifetime. Selectivity differences arise from the interactions of the different counter ions forms with the hydroxyl groups on the analyte molecules.
Saccharide | H+ | Ca2+ | Pb2+ |
|---|---|---|---|
| Adonitol | 11.5 | 14.9 | 20.4 |
| Arabinose | 11.4 | 13.6 | 19.4 |
| Erythritol | 12.7 | 15.6 | 20.3 |
| Fructose | 10.6 | 13.5 | 19.3 |
| Fucose | 12.2 | 13.7 | 17.1 |
| Galactose | 1.07 | 12.2 | 15.6 |
| Glucose | 9.9 | 11.1 | 13.9 |
| Glycerol | 14.1 | 16.1 | 19.5 |
| Lactose | 8.6 | 9.7 | 12.8 |
| Maltose | 8.4 | 9.5 | 12.5 |
| Maltotriose | 7.7 | 8.7 | 11.9 |
| Mannitol | 11.0 | 17.3 | 28.9 |
| Mannose | 1.5 | 12.5 | 16.7 |
| Raffinose | 8.2 | 8.6 | 11.4 |
| Sorbitol | 11.1 | 20.7 | N/A |
| Sucrose | 9.8 | 9.4 | 11.9 |
| Xylose | 10.6 | 12.0 | 15.0 |
HyperREZ XP Carbohydrate columns are based on a monodisperse resin with 4 or 8% divinylbenzene content, and provide an ideal medium for the analysis of carbohydrates and organic acids. Unlike silica based columns they are stable at low pH, allowing the use of dilute acid as a mobile phase. The columns can also be run at elevated temperatures for faster analysis and improved resolution of some closely eluting analytes. The columns can be easily regenerated for increased column lifetime. Selectivity differences arise from the interactions of the different counter ions forms with the hydroxyl groups on the analyte molecules.
Saccharide | H+ | Ca2+ | Pb2+ |
|---|---|---|---|
| Adonitol | 11.5 | 14.9 | 20.4 |
| Arabinose | 11.4 | 13.6 | 19.4 |
| Erythritol | 12.7 | 15.6 | 20.3 |
| Fructose | 10.6 | 13.5 | 19.3 |
| Fucose | 12.2 | 13.7 | 17.1 |
| Galactose | 1.07 | 12.2 | 15.6 |
| Glucose | 9.9 | 11.1 | 13.9 |
| Glycerol | 14.1 | 16.1 | 19.5 |
| Lactose | 8.6 | 9.7 | 12.8 |
| Maltose | 8.4 | 9.5 | 12.5 |
| Maltotriose | 7.7 | 8.7 | 11.9 |
| Mannitol | 11.0 | 17.3 | 28.9 |
| Mannose | 1.5 | 12.5 | 16.7 |
| Raffinose | 8.2 | 8.6 | 11.4 |
| Sorbitol | 11.1 | 20.7 | N/A |
| Sucrose | 9.8 | 9.4 | 11.9 |
| Xylose | 10.6 | 12.0 | 15.0 |
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