Designed for optimum chromatography
Enjoy the range and capabilities of these state-of-the-art columns and gain confidence in the accuracy and quality of your analytical data. The Thermo Scientific Hypersil GOLD family of liquid chromatography (LC) columns is the culmination of 40 years of experience in the media and column product development and manufacturing.
Outstanding peak shape results in greater sensitivity. When peaks exhibit tailing, peak height is reduced, therefore compromising the sensitivity of analysis. The highly symmetrical peaks provided by Hypersil GOLD columns enhance peak height and allow for optimized peak integration calculations. This can be particularly critical when low concentrations of an analyte are present, such as in an impurity assay.
Conditions
- Column: Hypersil GOLD, 5μm, 150 × 4.6mm
- Mobile Phase: A: 0.1% ammonia pH 10.6
- B: Methanol + 0.1% ammonia
- Gradient: 5–100% B in 15 min
- Flow Rate: 1.0mL/min
- Injection Volume: 10μL
- Detection: UV @ 254nm
- Temperature: 30 °C
| Phase | Phase Properties |
|---|---|
| Hypersil GOLD Peptide HPLC Columns | Consistent retention of all peptides, including hydrophilic ones, and resolve deamidated species |
| Hypersil GOLD C18 Selectivity LC Columns | Excellent peak shape for all analyte types. |
| Hypersil GOLD C8 LC Columns | Similar selectivity to C18 columns, but with reduced retention. |
| Hypersil GOLD C4 LC Columns | Similar selectivity to C18 and C8 columns, but with reduced retention. |
| Hypersil GOLD aQ LC Columns | Polar endcapped C18 columns, stable in 100% aqueous mobile phase, providing enhanced retention and resolution of polar analytes. |
| Hypersil GOLD PFP LC Columns | Perfluorinated phenyl columns offering alternative selectivity. The fluorine atoms around the phenyl ring enhance pi-pi interactions with aromatic molecules. |
| Hypersil GOLD Phenyl LC Columns | Excellent retention and unique selectivity for aromatic analytes. |
| Hypersil GOLD Cyano LC Columns | Alternative selectivity with lower hydrophobicity; can also be used for normal phase separations. |
| Hypersil GOLD Amino LC Columns | Can be used in reversed phase, normal phase, ion exchange, and HILIC modes; particularly useful for separating carbohydrates. |
| Hypersil GOLD AX LC Columns | Separation of smaller proteins, peptides, anionic species, and polar molecules. |
| Hypersil GOLD SAX LC Columns | A quaternary amino ion exchange ligand ideally suited to separating small polar organic analytes in aqueous mobile phases. |
| Hypersil GOLD Silica LC Columns | Separation of nonpolar and moderately polar organic compounds by normal phase chromatography. |
| Hypersil GOLD PEI HILIC LC Columns | Enhanced retention of polar and hydrophilic analytes that are problematic using reversed phase columns. |
Important product information
Hypersil GOLD columns are based on improved, highly pure silica and a novel proprietary derivatization and endcapping procedure using alkyl chain chemistry.
The capabilities of this state-of-the-art family of columns, with numerous chemistries and a range of particle sizes and hardware formats, meet the challenges of modern chromatography.
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