We offer Luciferase reporter reagents, multiple cloning site vectors, and flash, glow and dual-spectral luciferase assay kits based on the intracellularly expressed firefly luciferase. Firefly luciferase is a 60kDa protein produced in nature by several species of the Lampyridae family of beetles which includes the genera Photinus and Luciola. Bioluminescent signal from firefly luciferase originates from the oxidation of D-luciferin. Light output captured using a luminometer can be correlated with the amount of firefly luciferase protein produced and used to determine the activity of the promoter driving firefly expression.
Choose the right Firefly luciferase assay for your application
| Order now | Order now | Order now | |
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| Spectral Type | Single-spectral | Single-spectral | Single-spectral |
| Assay Type | Flash-type | Glow-type | Flash-type |
| Luciferase | Firefly | Firefly | Firefly |
| Description |
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| Average Half-Life | 30 min | >1 hr | >1 hr |
The Thermo Scientific Pierce Firefly Luciferase Flash Assay Kit provides reagents to measure Red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly.
The Thermo Scientific Pierce Firefly Luciferase Glow Assay Kit provides a bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of firefly luciferase reporters for protein expression assays.
Red firefly luciferase reporter assay vectors
Firefly luciferase reaction
For Research Use Only. Not for use in diagnostic procedures.