Bz-rA CEP
Designed for oligonucleotide synthesis for diagnostic and therapeutic development
Thermo Scientific RNA phosphoramidites are built to meet the varying needs of oligonucleotide manufacturers for the development of therapeutic, diagnostic, and research applications. We offer TheraPure, standard, and fast deprotect phosphoramidites.
- TheraPure phosphoramidites undergo additional quality control release testing and are compared with our standard phosphoramidites, helping to ensure that impurities and residual solvents are controlled to the stringent levels required by our customers for their oligo therapeutics.
- Standard phosphoramidites support the quality and reliability suitable for routine oligonucleotide synthesis.
- Fast deprotect phosphoramidites allow the removal of base protecting groups under mild or accelerated conditions without the need for special reagents.
Our RNA phosphoramidites are supported by:
- Expert technical support—over 40 years of partnership, customization, and scale-up experience
- Secure and consistent supply—5x capacity expansion in 2022
- Service excellence—48 hours or less response to orders on standard business days, >98% delivery to customer at promised date†
- Flexible packaging—custom and bulk packaging available upon request
- Quality—ISO 9001:2015–certified quality management system that has been refined in partnership with our customers since 1995
Quality manufacturing and regulatory documentation
Processes are monitored from material procurement through final QC and product release for batch-to-batch reproducibility. Purity of each phosphoramidite lot is assessed by HPLC-MS, GC, 31P NMR, as well as Karl Fischer oven titration. Our rigorously controlled manufacturing processes help minimize levels of reactive P(III) impurities which could be incorporated into the oligonucleotide during synthesis and result in the accumulation of by-products.
Available RNA phosphoramidites by synthesizer
Applied Biosystems/AKTA Synthesizer | Expedite Synthesizer | MerMade Synthesizer | Bulk | ||
---|---|---|---|---|---|
TheraPure phosphoramidites | Bz-rA | ✓ | ✓ | ✓ | ✓ |
Ac-rC | ✓ | ✓ | ✓ | ✓ | |
iBu-rG | ✓ | ✓ | ✓ | ✓ | |
rU | ✓ | ✓ | ✓ | ✓ | |
Standard phosphoramidites | Bz-rA | ✓ | |||
Ac-rC | ✓ | ||||
iBu-rG | ✓ | ||||
rU | ✓ | ||||
Fast deprotection (mild) | PAC-rA | ✓ | ✓ | ||
iPrPAC-rG | ✓ | ✓ | |||
Ac-rG | Contact us |
For items not listed above, flexible/bulk packaging, or custom services, please contact us ›
TheraPure RNA Phosphoramidites
TheraPure phosphoramidites are manufactured with high purity and tight control of critical impurity level for excellent batch-to-batch reproducibility.
Click images to enlarge
Bz-rA CEP | iBu-rG CEP | Ac-rC CEP | rU CEP | |
---|---|---|---|---|
27-1903 | 27-1906 | 27-1805 | 27-1804 | |
Formula | C53H66N7O8PSi | C50H68N7O9PSi | C47H64N5O9PSi | C45H61N4O9PSi |
Molecular weight | 988.2 g/mol | 970.1 g/mol | 902.1 g/mol | 861.1 g/mol |
CAS # | 104992-55-4 | 147201-04-5 | 121058-88-6 | 118362-03-1 |
Appearance | Color intensity must not exceed Pantone #P1-4U or #P4-2U | |||
Solution clarity | Free from undissolved particulate at 0.2 M solution in ACN | |||
HPLC purity | ≥99% | |||
31P NMR purity | ≥99% | |||
Residual solvent | ≤3.0% total % by weight solvent residue | |||
Shipping / Storage condition | Ambient / –20°C | |||
Water content | ≤0.3% | |||
Material origin | Non-animal source (no TSE/BSE risk) |
Ordering information
Not finding the product you need? Please contact us or request custom services.
Standard RNA Phosphoramidites
Traditional base protection methods are used to help ensure quality and reliability for routine oligonucleotide synthesis.
Click images to enlarge
Bz-rA CEP | iBu-rG CEP | Ac-rC CEP | rU CEP | |
---|---|---|---|---|
Cat. No. | 27-1403 | 27-1406 | 27-1405 | 27-1404 |
Formula | C53H66N7O8PSi | C50H68N7O9PSi | C47H64N5O9PSi | C45H61N4O9PSi |
Molecular weight | 988.2 g/mol | 970.1 g/mol | 902.1 g/mol | 861.1 g/mol |
CAS # | 104992-55-4 | 147201-04-5 | 121058-88-6 | 118362-03-1 |
Appearance | Color intensity must not exceed Pantone #P1-4U or #P4-2U | |||
HPLC purity | ≥98.0% | |||
31P NMR purity | ≥98.0% | |||
Shipping / Storage condition | Ambient / –20°C | |||
Water content | ≤0.3% | |||
Material origin | Non-animal source (no TSE/BSE risk) |
Ordering information
Not finding the product you need? Please contact us or request custom services.
Fast deprotect RNA phosphoramidites
They are ideal for applications requiring mild deprotection to help preserve the integrity of oligonucleotides containing sensitive dyes, labels, or other modifications that may be unstable with prolonged exposure to strong alkaline conditions. Fast deprotect phosphoramidites permit the removal of base protecting groups under mild or accelerated conditions without the need for special reagents.
Click images to enlarge
TheraPure PAC-rA CEP * | TheraPure iPrPAC-rG CEP * | TheraPure Ac-rG CEP ** | |
---|---|---|---|
Cat. No. | 27-1803 | 27-1806 | SY3000 |
Formula | C54H68N7O9PSi | C57H74N7O10PSi | C48H64N7O9PSi |
Molecular weight | 1018.2 g/mol | 1076.3 g/mol | 942.14 g/mol |
CAS # | 121058-86-4 | 956580-73-7 | 944138-03-8 |
Solution clarity | 0.2 M solution in ACN is free from undissolved particulate | ||
HPLC purity | ≥99% | ||
31P NMR purity | ≥99% | ||
Water content | ≤0.3% | ||
Shipping / Storage condition | Ambient / –20°C | ||
Material origin | Non-animal source (no TSE/BSE risk) |
942.14 g/mol |
942.14 g/mol |
** The acetyl-protected rG (Ac-rG, Cat No. SY3000) amidite is a suitable replacement for iBu-rG (Cat No. 2706) and is ideal for synthesis of longmers. Oligonucleotide deprotection times can be reduced as much as 25%.
Ordering information
Not finding the product you need? Please contact us or request custom services.
Technical note
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For Research Use Only. Not for use in diagnostic procedures.