DY547 CEP
Designed for oligonucleotide synthesis for diagnostic research and development
Dye-labeled phosphoramidites are intended for oligonucleotide synthesis designed for molecular assays, microscopy, and microarrays. A broad range of labeling options are available with coupling efficiency, emission, and absorption spectra comparable to other commercially available dyes.
These phosphoramidites may be used on automated DNA synthesizers following standard synthesis and deprotection procedures. Fluorescent tags can be incorporated at the 5′ end of an oligonucleotide, and DyLight dye–labeled phosphoramidites can be used in research applications without the constraints of licensing and royalty fees.
Our dye-labeled phosphoramidites are supported by:
- Technical expertise—over 40 years of partnership, customization, and scale-up experience
- Secure and consistent supply—5x capacity expansion in 2022 to support emerging therapeutics and diagnostic assays
- Service excellence—48 hours or less response to orders, >98% delivery to customer at promised date†
- Flexible packaging—custom and bulk packaging available upon request
- Refined quality—ISO 9001:2015–certified quality management system that has been refined in partnership with our customers since 1995
Quality manufacturing and documentation
Processes are monitored from material procurement through final QC and product release for batch-to-batch reproducibility. Purity of each phosphoramidite lot may be assessed by HPLC, NMR, and water determination by the Karl Fischer method. Our rigorously controlled manufacturing processes help minimize levels of reactive impurities which could be incorporated into the oligonucleotide during synthesis and result in the accumulation of by-products.
DyLight-labeled phosphoramidites
Thermo Scientific DyLight (DY) fluorescent labels offer choices with spectrally distinct colors for oligonucleotide labeling with ß-cyanoethyl phosphoramidites (CEP).
Chemical structures and technical specifications
DY547 CEP | DY647 CEP | DY677 CEP | |
---|---|---|---|
Cat. No. | SY6332 | SY6334 | SY6336 |
Formula | C38H54IN4O2P | C47H63N4O5PS | C55H67N4O5PS |
Molecular weight | 756.74 g/mol | 827.07 g/mol | 927.18 g/mol |
HPLC purity | ≥90% at 547 nm | ≥85% at 647 nm | ≥85% at 677 nm |
Identity | UV-Vis spectrum conforms to that of the authentic sample | ||
Shipping / storage condition | Dry ice / –20°C | ||
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Additional dye-labeled phosphoramidites
Phosphoramidites with fluoresceine and its derivatives like 6-carboxyfluorescein (6-FAM), hexachlorofluorescein (HEX), tetrachlorofluorescein (TET) are also available to provide you with flexibility in oligo labeling.
Chemical structures and technical specifications
Click images to enlarge
Fluorescein CEP | 6-FAM CEP | HEX CEP | TET CEP | ||
---|---|---|---|---|---|
Cat. No. | 27-1606 | 27-1608 | SY6342 | SY6341 | |
Chemical name | [(3′,6′-dipivaloylfluoresceinyl)-5,6-carboamidohexyl]-1-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | Fluorescein dipivalate phosphoramidite, 6-isomer | 6-(4,7,2′,4′,5′,7′-hexachloro-3′,6′-dipivaloylfluoresceinyl-6-carboxamido)-hexyl-1-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | 5′-tetrachloro-fluorescein phosphoramidite | |
Formula | C46H58N3O10P | C46H58N3O10P | C46H52N3O10Cl6P | C46H54N3O10Cl4P | |
Molecular weight | 843.95 g/mol | 843.94 g/mol | 1050.63 g/mol | 981.73 g/mol | |
CAS / PubChem# | N/A | 204697-37-0 / 58114954 | 1360547-55-2 | 877049-90-6 | |
HPLC purity | ≥90% | ≥90% | ≥95% | ≥95% | |
31P NMR purity | 85% | ≥95% (6-isomer) | N/A | N/A | |
Shipping / storage condition | Dry ice / –20°C | ||||
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For flexible/bulk packaging, custom services, quotes, or items not listed above, please contact us or submit your inquiry.
Technical note
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For Research Use Only. Not for use in diagnostic procedures.