Updated Cancer research guidebook
Learn how to maximize funding, save time, save precious samples, and more.
With over 100 subtypes, cancer is among the leading causes of death worldwide. Its dynamic nature presents an enormous scientific and clinical challenge for future discoveries. Whether it’s detecting biomarkers early or deterring its migration to other sites, thousands of cancer researchers around the world are on the front lines, working to discover cures and preventive treatments. We spoke with many of them to understand how we can help make their journey easier.
As part of that effort, we identified six common areas where we could help circumvent challenges:
- New techniques and education are needed
- Data are too limited
- Collaboration is limited
Updated Immuno‑oncology (I‑O) guide
Discover key I‑O research approaches, including checkpoint inhibitor therapy research, CAR T cell therapy research, and cancer vaccine therapy research.
Our commitment to you
We understand what cancer researchers face. Our goal is to support you with a comprehensive range of technologies and solutions that help you maximize your time, budget, data, and resources—accelerating your path from discovery to potential clinical translation. Discover educational resources and solutions that can help you overcome these impediments. Learn about how to maximize funding, save time, save precious samples and more. We’re committed to partnering with you for your next breakthrough.
Time is too precious—scientists shouldn’t have to spend excessive time creating and testing new protocols and workflows. Achieving efficiency shouldn’t come at the expense of accuracy either. New technologies have led to products that are designed to work together for rapid and reliable results.
Whether your cancer research would benefit from cutting-edge technologies or workflow efficiencies, utilizing time-saving products could make all the difference in the speed of research.
Featured cell analysis products
Save time with the high-performance capabilities of the Invitrogen Attune NxT Flow Cytometer. This dynamic instrument is designed with active mechanical resistance to clogging, novel acoustics-assisted hydrodynamic focusing for speed, and sample acquisition from 96-well and 384-well microtiter plates for reliable automation.
Immunophenotyping analysis on minimally processed samples can be performed using a simplified no-wash, no-lyse method, reducing the time-intensive traditional sample preparation protocols from 10 steps to 3.
Detection of cell populations can also be accomplished with CellTrace cell proliferation kits that permanently label cells with fluorescent stains to trace generations or divisions without affecting morphology or physiology.
Achieve exceptional results from immunophenotyping with our highly referenced intracellular fixation and permeabilization buffers. Remove dead cells and unwanted artifacts during analysis using our LIVE/DEAD fixable viability dyes.
Make the ELISA process easier and more reliable, reducing the typical workflow by 30% while maintaining the performance you require. With Invitrogen instant ELISA kits, there is no laborious preparation of reagents, serial dilutions of standards, or sequential additions to the plate. These kits are an excellent option for labs requiring a streamlined ELISA system that doesn’t compromise on accuracy.
One microplate reader for several applications and all skill sets. Varioskan LUX supports most common measurement technologies including: absorbance (UV-Vis, including pathlength correction), fluorescence intensity (including FRET), and luminescence to support a variety of plate-based assays eliminating one at a time measurements.
Measuring cell proliferation is a fundamental method for assessing cell health, determining genotoxicity, and evaluating anticancer drugs. We offer a wide variety of Invitrogen functional reagents to support cancer research and >13,000 primary antibody conjugates for flow cytometry.
Assays provide fast detection of DNA synthesis, in as little as 60 minutes, as an alternative to the cumbersome BrdU assay
Invitrogen ProQuantum immunoassay kits enable highly sensitive measurements of specific targets such as IFNγ, IL-8, and IL-6 using only a fraction of sample volume and with greater dynamic range compared to traditional methods. These assays offer a simplified workflow with analysis using qPCR technology.
Correct for overlapping fluorescence emission spectra using our Invitrogen UltraComp eBeads compensation beads. Additionally, Ready Flow ready-to-use flow cytometry reagents provide time-saving, and simplified protocols that do not require dilutions, calculations, or pipetting. These room temperature–stable reagents are available for most common needs, including dead cell identification, cell cycle analysis, and apoptosis.
Revolutionize your way to unveil the complex interactions in studying cell signaling pathways. The Invitrogen ProcartaPlex Signaling Dual Reporter assay format leverages the dual reporter capabilities of the Luminex xMAP INTELLIFLEX DR-SE instrument and enables the simultaneous detection of total and phosphorylated proteins in a single well within 4 hours.
Featured sample prep productsThe Thermo Scientific KingFisher Flex Purification System provides plate-based handling (mix, capture, transfer, release) of magnetic particles for bead-based nucleic acid and protein affinity purification procedures. This easy-to-use instrument provides excellent reproducibility and minimizes the hands-on time required for DNA, RNA, and protein isolation from a wide range of sample types. The system is available with four different user-interchangeable magnetic head formats, enabling scientists to process a variety of volumes with compatible purification reagents, such as Applied Biosystems and MagMAX nucleic acid extraction products.
Invitrogen Dynabeads magnetic beads are the most cited products for sensitive applications such as chromatin immunoprecipitation (ChIP) and immunoprecipitation (IP) of low-abundance proteins. These highly uniform particles reduce nonspecific binding and enable IP protocols to be performed in less than 40 minutes. Automated IP protocols are now available for use of Dynabeads products with KingFisher systems.
Featured amplification productsAchieve faster and more efficient PCR with a unique universal primer annealing feature that allows for co-cycling of all assays, reducing tedious optimization steps. Invitrogen Platinum II Taq Hot-Start DNA Polymerase has also been engineered to synthesize DNA up to 4X faster than standard Taq polymerase, reducing PCR run times.
Combine the convenience of rapid, real-time nucleic acid analysis with the precision of high-resolution image capture to reduce workflow time and accelerate discovery. Using the E-Gel Power Snap Electrophoresis System with precast E-Gel agarose gels can simplify the nucleic acid electrophoresis workflow. There are no gels to pour, buffers to make, staining or destaining steps to perform, or gel boxes to assemble. Just load your samples and run.
Featured nucleic acid analysis productsIon Torrent Oncomine cell-free nucleic acid assays are multibiomarker next-generation sequencing (NGS) assays that enable reproducible detection of somatic mutations in plasma, down to a level of 0.1% in genes relevant to solid tumors. These assays are designed to use a single tube of blood for analysis of SNVs, short indels, CNVs, and fusions that are frequently mutated in cancer research samples. When used as part of a complete NGS workflow, the process from blood research sample to variant data takes as little as two days.
Semiconductor sequencing instruments enable the production of high-quality data in just a few hours. With the Ion GeneStudio S5 systems, you can go from DNA library to data in as little as 24 hours, with only 45 minutes of hands-on time, when paired with the Ion Chef System.
Featured protein analysis productsiWestern workflow for western blot analysis
Designed to address processing efficiency, reproducibility, and robustness of results, the Invitrogen iWestern workflow products enable exceptional western blotting results with minimal hands-on time, allowing you to focus on what matters most—your data.
Reliable tools and reagents help cancer researchers achieve more with limited funding. From antibodies that are proven to perform, to tools that work better together, solutions that can help maximize funding is mission critical. The following core solutions for cancer research may be your biggest opportunity to achieve cost efficiency.
Featured cell analysis products
Cancer researchers can’t afford to take risks with challenging samples. Invitrogen Alexa Fluor Plus secondary antibodies represent an advancement in fluorescent-conjugate technology, designed to provide brighter signals, enhanced sensitivity, and minimal cross-reactivity in a variety of samples and applications. Make your low-abundance targets visible, spend less time optimizing, and make every one of your precious samples count with antibodies that perform.
Customize experiments with up to 4 lasers and 14 colors with options for up to 6 channels off of the violet laser. Get the freedom to build multicolor panels, accommodating common fluorophores and dyes.
Leverage a new platform that enables measurement of target-specific proteins such as IFNγ, IL-8, and IL-6. Invitrogen ProQuantum immunoassay kits utilize proximity ligation assay (PLA) technology and 2 μL of sample or less per run. Unlike other next-generation immunoassay platforms on the market, you do not need to buy an additional, closed system. ProQuantum immunoassays can be run on any existing qPCR instrument, which maximizes the value of the instruments you already have.
Invitrogen uncoated ELISA kits are ideal for experienced ELISA users on a budget, with less stringent requirements for inter- and intra-assay variance. These kits are several times more economical than ready-to-use ELISA kits that contain precoated plates.
Recover high percentages of nucleic acids from your samples and maximize your budget with TRIzol reagent for RNA and DNAzol reagent for genomic DNA. These organic reagents are well established. TRIzol has been references in over 70,000 publications. Extraction of RNA can be completed in under an hour. Genomic DNA extraction with DNAzol can be completed in 10-30 minutes with recoveries of 70-100%.
The Applied Biosystems SimpliAmp Thermal Cycler delivers high performance at an affordable price. The 96-well VeriFlex block has 3 independent temperature zones, for precise temperature control over your PCR optimization. The SimpliAmp Thermal Cycler also features an 8-inch color touch screen and is WiFi enabled for remote monitoring.
Backed by the proven reliability of Applied Biosystems technology, MiniAmp and MiniAmp Plus thermal cyclers were designed for routine PCR. With their compact design at just 7.5 inches (19 cm) wide and entry-level prices, these thermal cyclers are perfect for every lab bench.
Featured cell culture products
Benefit from a proven combination of Gibco cell culture media and Thermo Scientific Nunc plastics for consistent cell growth. Every lot of Nunc plastics with the Nunclon Delta cell culture–treated surface is verified with trusted Gibco media to confirm consistent cell growth across multiple cell lines. Only Thermo Scientific cell culture plastics endure rigorous testing with Gibco media, including cloning efficiency tests using specially selected cell lines with high sensitivity to toxic components. It’s the perfect pairing that can mean the difference between success and starting over.
We’re committed to helping you maximize every precious research sample. Feel reassured by tools that provide enhanced sensitivity so that you can accelerate your research path forward. From reagents to kits to instruments, our solutions are designed to empower your next discovery.
Featured cell analysis products
Study up to 16-data parameters from your precious samples while saving time with the high-performance capabilities of the Invitrogen Attune NxT Flow Cytometer. This dynamic instrument is designed with active mechanical resistance to clogging, novel acoustics-assisted hydrodynamic focusing for speed, and sample acquisition from 96-well and 384-well microtiter plates for reliable automation. Immunophenotyping analysis on minimally processed samples can be performed using a simplified no-wash, no-lyse method, reducing the time-intensive traditional sample preparation protocols from 10 steps to 3 straightforward steps.
Invitrogen CytoVista Tissue Clearing Reagent sufficiently clears tissue for 3D fluorescent imaging, but unlike other clearing techniques does not significantly change the tissue morphology. Minimal morphological changes, such as shrinkage and contraction, have been observed. For precious samples, the clearing can be reversed, and the tissue can be processed for histology applications such as hematoxylin and eosin (H&E) staining.
The Invitrogen PureLink RNA Micro Scale Kit is designed to be used when input is limited. It provides rapid purification and concentration of total RNA from a variety of samples, including LCM tissues, fine-needle aspirates, and sorted cells.
Unlocking the information contained in FFPE samples is essential to cancer research. The Applied Biosystems MagMAX FFPE DNA/RNA Ultra Kit is designed for sequential isolation of DNA and RNA from the same FFPE tissue sample, with a minimal requirement of a 5 μm section or curl. The DNA and RNA are recovered in separate eluates, and both are compatible with a broad range of applications, including real-time PCR and NGS.
Invitrogen SYBR Gold Nucleic Acid Gel Stain is the most sensitive fluorescent stain we offer for nucleic acid detection. Maximize your precious DNA and RNA samples with enhanced sensitivity to detect as little as 25 pg of sample.
Invitrogen Platinum SuperFi DNA Polymerase is a proofreading enzyme that combines superior fidelity with trusted Platinum hot-start technology. Featuring >100x the fidelity of Taq polymerase, Platinum SuperFi DNA Polymerase is ideally suited for applications that benefit from exceptional sequence accuracy. In addition, the extreme robustness of the polymerase enables successful amplification of samples of suboptimal purity.
With simple cartridge-loaded reagents and a straight-forward user interface, the Ion GeneStudio S5 System makes NGS fast and easy—ideal for any cancer research lab. Minimize sample input and maximize flexibility. Ion AmpliSeq technology enables successful library preparation from challenging sample types such as formalin-fixed, paraffin-embedded (FFPE) tissue, retrospective samples from fine-needle aspirates, and cell-free DNA (cfDNA) extracted from blood, using as little as 1 ng of input DNA or RNA to support multiple applications. With this system, you can efficiently implement small and large projects across multiple research applications with a simple sample-to-data NGS workflow.
Applied Biosystems TaqMan Advanced miRNA assays enable highly sensitive and specific quantitation of mature miRNAs using qPCR. Together with the TaqMan Advanced miRNA cDNA Synthesis Kit, this solution provides a streamlined workflow that allows for the detection and quantitation of mature miRNA from as little as 1 pg of total RNA or 2 μL of plasma or serum.
Invitrogen SuperScript IV Reverse Transcriptase offers superior cDNA synthesis in a short 10-minute reaction. It is designed to provide reliable and consistent results even with the most challenging RNA samples.
The Ion Torrent Oncomine TCR Beta-LR Assay is designed to efficiently capture all three complementarity determining regions of the TCR beta chain gene (CDR1, CDR2, CDR3) with as little as 10ng of RNA input. This assay enables key applications such as predictive or prognostic biomarker discovery, T cell characterization, and identification of variable gene polymorphisms from samples such as RNA extracted from whole blood, fresh-frozen tissue, or sorted cells. The use of RNA template allows sequencing of productive and relevant variable (V), diversity (D), and joining (J) rearrangements—improving the identification of rare clones.
With a 3-day workflow using as little as 20 ng of tumor DNA, the Ion Torrent Oncomine Tumor Mutation Load Assay covers 1.65 Mb across 409 oncogenes relevant to major cancer types. The assay highly correlates with exome mutation counts and obviates the need for whole-exome sequencing, allowing for a higher percentage of samples to be evaluated and conserving precious samples for additional biomarker assessment. Additionally, our Oncomine informatics enable simultaneous assessment of TMB and variant profiling for greater actionable insights.
The Applied Biosystems Arcturus Cellect LCM System is optimized for applications ranging from single-cell isolation to extraction of small cell populations with a high degree of precision and flexibility. The instrument enables researchers to uncover unique molecular signatures that may be obscured in heterogeneous tumor samples. This system features the unique combination of a gentle infrared (IR) laser to capture the cells of interest and a powerful ultraviolet (UV) laser for efficient microdissection.
Applied Biosystems TaqMan Mutation Detection assays are powered by allele-specific TaqMan PCR (castPCR) technology to detect and measure somatic mutations in genes of interest to cancer research. This highly specific and sensitive technology can detect rare copies of mutated DNA in a sample that contains large amounts of wild type DNA—down to 1 cancer cell in 1,000 normal cells. These assays are compatible with sample types such as cell lines, FFPE tissue, and fresh-frozen tissue.
The Invitrogen Qubit Fluorometer is designed to quickly and specifically quantitate DNA or RNA. In addition, with the newest version of the Qubit Fluorometer, you can now easily measure RNA integrity and quality. It is now easier than ever to make sure you have enough DNA or RNA for your experiment. Use as little as 1 μL of sample, even with very dilute samples. Dyes in Invitrogen Qubit assays bind selectively to DNA, RNA, or protein, making them more sensitive than UV absorbance.
When working with challenging RNA samples or analyzing low-abundance transcripts, the ability of a reverse transcriptase to generate cDNA from the lowest amount of input RNA is crucial. Invitrogen SuperScript IV VILO Master Mix offers highly sensitive and robust cDNA synthesis for RT-qPCR applications in a short 10-minute reaction. Obtain higher cDNA yields and earlier Ct values (up to 6 cycles ahead of other reverse transcriptases), all while maintaining superior linearity across a broad range of RNA inputs. Additionally, superior inhibitor resistance allows for the utmost confidence in results, even with degraded or inhibitor-containing RNA samples.
Requiring low sample input, the Ion Torrent Oncomine TCR Beta-SR Assay specifically interrogates the CDR3 region of the TCR beta chain gene. Compatible with both FFPE DNA and RNA, this short-read sequencing assay enables characterization of immune status and detection of T cell clones in peripheral blood. The assay offers a 2-day turnaround time and is complete with superior informatics for accurate clonality and sequence assessment of the CDR3 region without interference from primer bias.
Clariom D Pico and Clariom S Pico microarray assays
Many expression profiling techniques require large amounts of input RNA, which are often not available in cancer research samples. Applied Biosystems Clariom Pico assays can generate detailed, robust, and accurate data from as little as 100 pg of total RNA (as few as 10 cells) from a wide range of challenging clinical research samples.
Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultrasensitive enhanced chemiluminescent (ECL) substrate for low-femtogram detection of protein by western blot analysis with the enzyme horseradish peroxidase (HRP). This substrate produces a quantitative signal that is easily captured by exposure to film or with an imaging system. The substrate offers excellent signal duration—8 hours of usable light output when conditions are optimized.
Invitrogen Bolt Bis-Tris Plus mini gels are designed for optimal separation of a broad molecular weight range of proteins under denaturing conditions during gel electrophoresis. The unique WedgeWell design allows loading of up to two times more sample volume than other precast gels. Bolt gels are ideal for western blot analysis and applications where protein integrity is crucial and higher sensitivity is required.
Featured cell culture products
Gibco extracellular matrix (ECM) products are essential tools for the growth, differentiation, and maturation of 2D and 3D cellular models. Products including Gibco Geltrex matrix, collagen, and the AlgiMatrix system are widely used to provide cells with more physiologically relevant environments that promote in vivo–like morphology. Lot-to-lot consistency and functional testing help provide increased confidence for specialized cell types like stem cells or cancer cell lines.
Techniques for gene manipulation, expression analysis, and cancer cell modeling can be difficult to implement in the lab. New technologies can help simplify these techniques and make them accessible to all researchers. This increased accessibility leads to broader adoption by the research community, allowing the techniques to be standardized over time. From nucleic acids to proteins to cells, empower your cancer research workflow.
The Thermo Scientific KingFisher Flex Purification System provides plate-based handling (mix, capture, transfer, release) of magnetic particles for bead-based nucleic acid and protein affinity purification procedures. This easy-to-use instrument provides excellent reproducibility and minimizes the hands-on time required for DNA, RNA, and protein isolation from a wide range of sample types and volumes.
Liquid biopsy is a noninvasive technique for molecular biomarker discovery that is expected to eliminate the need for costly, invasive procedures. Our wet lab–verified Applied Biosystems TaqMan Liquid Biopsy dPCR assays enable the sensitive detection and quantitation of low-frequency mutations, including the most common cancer-related mutations in EGFR, BRAF, KRAS, PIK3CA, JAK2, and other genes.
Invitrogen QuantiGene RNA assays are hybridization-based assays that utilize branched DNA signal amplification technology to measure gene expression. QuantiGene assays work directly with cell or blood lysates or tissue homogenates without RNA purification or enzymatic manipulation. Invitrogen QuantiGene Plex DNA CNV assays are also available to measure differences in copy number.
The Invitrogen PrimeFlow RNA Assay expands the capability of flow cytometry to measure RNA. It is the only known commercial assay that enables the simultaneous detection of up to four RNA targets in combination with immunophenotyping for cell-surface and intracellular proteins using fluorophore conjugated antibodies. The PrimeFlow RNA Assay can detect mRNA, lncRNA, and microRNA, as well as viral RNA and telomere DNA.
The Ion Torrent Oncomine Focus Assay is a targeted, multibiomarker assay that enables you to target hotspots, SNVs, indels, CNVs, and gene fusions in DNA and RNA in a single workflow. Designed for clinical and translational cancer research, the Oncomine Focus Assay leverages Ion AmpliSeq technology producing robust results, low input amounts, and analysis of low-quality samples, and is compatible with FFPE fine-needle aspirates and core-needle biopsies.
Current methods for the analysis of hematological malignancies often involve multiple sequential tests and laborious workflows. The Ion Torrent Oncomine Myeloid Research Assay is a comprehensive, targeted NGS assay designed to assist in the understanding of myeloid cancer in a single test. It interrogates relevant DNA mutations and fusion transcripts associated with myeloid disorders in a quick and easy NGS assay.
The Invitrogen Collibri library preparation kits and accessories generate libraries from many sample types including FFPE. Libraries are prepared more quickly, with less bias. Collibri library prep kits have incorporated the following features: visual feedback for higher library prep success, and shorter total workflow times.
Featured cell culture products
As you move from basic cell therapy research to the clinic, high-quality products and proper documentation are essential to getting it right the first time. Cell Therapy Systems (CTS) products help minimize the risk of contamination and variability in your research and provide the required documentation for regulatory review.
In cancer research, there is simply no room for error. Getting robust and reliable data from a single sample is critical to advancing your cancer research studies. Simple and efficient ways to share data can accelerate productivity and enhance collaboration. Learn how cloud-enabled tools are helping cancer researchers achieve better productivity, improved data, and streamlined data sharing.
Featured cell analysis products
The Thermo Scientific CellInsight CX7 LZR High Content Analysis Platform is a fast, laser-based, automated cellular imaging and analysis platform for quantitative microscopy and phenotypic classification. It’s designed to provide you with the sensitivity and speed that’s needed for emerging assays. HCS Studio Cell Analysis Software offers intuitive interfaces and guided workflows for integrated data collection and analysis on CellInsight platforms.
The unique design of the Invitrogen Attune NxT Flow Cytometer offers progressive application capabilities, from 1 to 4 lasers and from 4 to 14 colors, providing expanded assay development flexibility and increasing the information you can obtain from your sample. This translates into more data with better population identification than if you were using fewer parameters.
The Applied Biosystems CytoScan HD Suite replaces the need to run multiple tests on multiple technologies to obtain comprehensive cytogenetic analysis of hematological malignancy samples. With the Cytoscan HD Suite solution you can detect chromosomal arm aberrations, focal changes, loss of heterozygosity (LOH), copy-neutral loss of heterozygosity (cnLOH), ploidy changes, chromothripsis, and more.
Get more RNA expression data from a single assay and expand your potential to discover novel, informative cancer biomarkers with Applied Biosystems Clariom D assays. Our novel microarray-based Clariom D Pico assays provide intricate transcriptome-wide gene and exon-level expression profiles, including the ability to detect alternative splicing events of coding RNA and long noncoding RNA (lncRNA) in a single 3-day experiment.
Minimize the barriers of next-generation sequencing (NGS) data analysis and simplify your bioinformatics path to discovery. Ion Reporter Software provides an optimized suite of simple tools that streamline data analysis for Ion GeneStudio S5, Ion PGM, and Ion Proton systems. With these tools, you can focus on finding the biological meaning of your data, share your findings with other researchers safely, and spend less time on configuring the software.
The types of genetic aberrations associated with childhood malignancies differ from those affecting the adult population, so a specifically designed, targeted but comprehensive panel is needed to advance clinical research and drive future therapeutic solutions for precision oncology. The Ion Torrent Oncomine Childhood Cancer Research Assay is a unique, NGS-based tool designed for comprehensive genomic profiling of cancers affecting children and young adults.
Reliably detect differences in gene expression as small as 1.5 fold on the Applied Biosystems QuantStudio 6 and 7 Pro real-time PCR systems. Multiplexing of 5 or 6 optical channels is available to gain more results from your most precious samples.
Monitor, analyze and share data from wherever you are using our connect cloud-cloud based platform and app. These systems are compatible with automation and TaqMan array plates with RFID to increase convenience and reduce manual errors.
See more copy number changes in your cancer research samples with the Applied Biosystems OncoScan CNV Assay. Designed to cover the whole genome as well as known cancer-driver events, this single microarray-based assay easily detects copy number gains and losses, LOH, cnLOH, ploidy, allele-specific changes, break points, mosaicism, clonal heterogeneity, and chromothripsis.
The Ion Torrent Oncomine Comprehensive Assay v3 is a member of the family of widely adopted Oncomine assays for clinical cancer research. Oncomine assays are NGS-based multi-biomarker assays used to profile thousands of samples in different translational and clinical research projects, and have consistently delivered reliable results.
The Ion Torrent Oncomine Immune Response Research Assay is a targeted NGS gene expression assay that enables immunotherapy research from the quantitative evaluation of the expression of markers associated with different leukocyte subsets, antigen presentation, checkpoint pathways, and tumor progression. This 400-gene assay is supported with a user-friendly informatics workflow and demonstrates high sensitivity for low-expressing transcripts derived from FFPE samples.
The Ion Torrent Oncomine BRCA Research Assay for the detection of BRCA somatic and germline mutations in formalin-fixed, paraffin-embedded (FFPE) tissue and whole blood has been widely adopted in molecular pathology research laboratories across the world. The assay can detect large insertions and deletions (indels) and exon or whole-gene deletion or duplication events, uniquely empowering laboratories to detect all classes of mutations in one NGS workflow.
Capture images and analyze data from your western blots and gels efficiently and easily using Invitrogen iBright imaging systems. These high-performance instruments enhance the imaging experience through advanced automated features and an interface that is easy to use for researchers of all experience levels.
Featured cell culture products
Invitrogen CytoVista 3D Cell Culture Clearing Reagent clears fluorescently labeled 3D cultured cells such as organoids and spheroids. This clearing process helps improve data quality by enabling sharp and bright 3D fluorescent imaging for depths up to 1,000 μm. The reagent can also be used as an imaging solution, with stable storage of 3D cell cultures for weeks with minimal change in tissue morphology, or brightness or specificity of the fluorophores.
Together, we can be smarter, faster, and more determined than ever in the fight against cancer. Collaboration is the key to efficiency. We strive to bring the research community together through impactful initiatives, virtual conferences, and live events. The collaborative spirit has evolved to become stronger and even more connected. Discover how we are helping researchers stay connected.
Featured cell analysis products
Experimental reproducibility is vital for the advancement of cancer research. However, the replication of studies has long been a challenge due to the lack of characterization standards for antibodies (Figure 27). Everyone from the scientific community plays a role in this challenge: suppliers, researchers, and publishers. We can increase reproducibility by collaborating in three important ways:
- Research—verify your antibody and your experimental protocol
- Publish—use and list your controls and standards along with details about antibodies and experimental methods
- Train—expand your knowledge about experimental methods and controls, sample preparation, and other aspects of properly using antibodies
Thermo Fisher Scientific has implemented methods for the verification and reporting of antibody specificity to further enhance data transparency. These specificity tests help ensure that the antibody will bind to the correct target.
Reliably detect differences in gene expression as small as 1.5 fold on the Applied Biosystems QuantStudio 6 and 7 Pro real-time PCR systems. Multiplexing of 5 or 6 optical channels is available to gain more results from your most precious samples.
Monitor, analyze and share data from wherever you are using our connect cloud-cloud based platform and app. These systems are compatible with automation and TaqMan array plates with RFID to increase convenience and reduce manual errors.
Community empowerment for mass spectrometry (MS) users comes in the form of the Thermo Scientific Tandem Mass Tag (TMT) Research Award and the ProteomicsNOW MS Virtual Event. For research users of Thermo Scientific TMT labeling reagents (including amine-, sulfhydryl-, or carbonyl-reactive reagents), you could receive one of three awards. Terms & conditions
Featured cell culture products
Gibco Cell Culture Heroes spotlights PhD students and postdoctoral scientists across the globe with the primary focus of telling the story of their cancer research. This program promotes education and creates a growing community of researchers who are driving tomorrow’s breakthroughs in the fight against cancer.
Time is too precious—scientists shouldn’t have to spend excessive time creating and testing new protocols and workflows. Achieving efficiency shouldn’t come at the expense of accuracy either. New technologies have led to products that are designed to work together for rapid and reliable results.
Whether your cancer research would benefit from cutting-edge technologies or workflow efficiencies, utilizing time-saving products could make all the difference in the speed of research.
Featured cell analysis products
Save time with the high-performance capabilities of the Invitrogen Attune NxT Flow Cytometer. This dynamic instrument is designed with active mechanical resistance to clogging, novel acoustics-assisted hydrodynamic focusing for speed, and sample acquisition from 96-well and 384-well microtiter plates for reliable automation.
Immunophenotyping analysis on minimally processed samples can be performed using a simplified no-wash, no-lyse method, reducing the time-intensive traditional sample preparation protocols from 10 steps to 3.
Detection of cell populations can also be accomplished with CellTrace cell proliferation kits that permanently label cells with fluorescent stains to trace generations or divisions without affecting morphology or physiology.
Achieve exceptional results from immunophenotyping with our highly referenced intracellular fixation and permeabilization buffers. Remove dead cells and unwanted artifacts during analysis using our LIVE/DEAD fixable viability dyes.
Make the ELISA process easier and more reliable, reducing the typical workflow by 30% while maintaining the performance you require. With Invitrogen instant ELISA kits, there is no laborious preparation of reagents, serial dilutions of standards, or sequential additions to the plate. These kits are an excellent option for labs requiring a streamlined ELISA system that doesn’t compromise on accuracy.
One microplate reader for several applications and all skill sets. Varioskan LUX supports most common measurement technologies including: absorbance (UV-Vis, including pathlength correction), fluorescence intensity (including FRET), and luminescence to support a variety of plate-based assays eliminating one at a time measurements.
Measuring cell proliferation is a fundamental method for assessing cell health, determining genotoxicity, and evaluating anticancer drugs. We offer a wide variety of Invitrogen functional reagents to support cancer research and >13,000 primary antibody conjugates for flow cytometry.
Assays provide fast detection of DNA synthesis, in as little as 60 minutes, as an alternative to the cumbersome BrdU assay
Invitrogen ProQuantum immunoassay kits enable highly sensitive measurements of specific targets such as IFNγ, IL-8, and IL-6 using only a fraction of sample volume and with greater dynamic range compared to traditional methods. These assays offer a simplified workflow with analysis using qPCR technology.
Correct for overlapping fluorescence emission spectra using our Invitrogen UltraComp eBeads compensation beads. Additionally, Ready Flow ready-to-use flow cytometry reagents provide time-saving, and simplified protocols that do not require dilutions, calculations, or pipetting. These room temperature–stable reagents are available for most common needs, including dead cell identification, cell cycle analysis, and apoptosis.
Revolutionize your way to unveil the complex interactions in studying cell signaling pathways. The Invitrogen ProcartaPlex Signaling Dual Reporter assay format leverages the dual reporter capabilities of the Luminex xMAP INTELLIFLEX DR-SE instrument and enables the simultaneous detection of total and phosphorylated proteins in a single well within 4 hours.
The Thermo Scientific KingFisher Flex Purification System provides plate-based handling (mix, capture, transfer, release) of magnetic particles for bead-based nucleic acid and protein affinity purification procedures. This easy-to-use instrument provides excellent reproducibility and minimizes the hands-on time required for DNA, RNA, and protein isolation from a wide range of sample types. The system is available with four different user-interchangeable magnetic head formats, enabling scientists to process a variety of volumes with compatible purification reagents, such as Applied Biosystems and MagMAX nucleic acid extraction products.
Invitrogen Dynabeads magnetic beads are the most cited products for sensitive applications such as chromatin immunoprecipitation (ChIP) and immunoprecipitation (IP) of low-abundance proteins. These highly uniform particles reduce nonspecific binding and enable IP protocols to be performed in less than 40 minutes. Automated IP protocols are now available for use of Dynabeads products with KingFisher systems.
Achieve faster and more efficient PCR with a unique universal primer annealing feature that allows for co-cycling of all assays, reducing tedious optimization steps. Invitrogen Platinum II Taq Hot-Start DNA Polymerase has also been engineered to synthesize DNA up to 4X faster than standard Taq polymerase, reducing PCR run times.
Combine the convenience of rapid, real-time nucleic acid analysis with the precision of high-resolution image capture to reduce workflow time and accelerate discovery. Using the E-Gel Power Snap Electrophoresis System with precast E-Gel agarose gels can simplify the nucleic acid electrophoresis workflow. There are no gels to pour, buffers to make, staining or destaining steps to perform, or gel boxes to assemble. Just load your samples and run.
Ion Torrent Oncomine cell-free nucleic acid assays are multibiomarker next-generation sequencing (NGS) assays that enable reproducible detection of somatic mutations in plasma, down to a level of 0.1% in genes relevant to solid tumors. These assays are designed to use a single tube of blood for analysis of SNVs, short indels, CNVs, and fusions that are frequently mutated in cancer research samples. When used as part of a complete NGS workflow, the process from blood research sample to variant data takes as little as two days.
Semiconductor sequencing instruments enable the production of high-quality data in just a few hours. With the Ion GeneStudio S5 systems, you can go from DNA library to data in as little as 24 hours, with only 45 minutes of hands-on time, when paired with the Ion Chef System.
iWestern workflow for western blot analysis
Designed to address processing efficiency, reproducibility, and robustness of results, the Invitrogen iWestern workflow products enable exceptional western blotting results with minimal hands-on time, allowing you to focus on what matters most—your data.
Reliable tools and reagents help cancer researchers achieve more with limited funding. From antibodies that are proven to perform, to tools that work better together, solutions that can help maximize funding is mission critical. The following core solutions for cancer research may be your biggest opportunity to achieve cost efficiency.
Featured cell analysis products
Cancer researchers can’t afford to take risks with challenging samples. Invitrogen Alexa Fluor Plus secondary antibodies represent an advancement in fluorescent-conjugate technology, designed to provide brighter signals, enhanced sensitivity, and minimal cross-reactivity in a variety of samples and applications. Make your low-abundance targets visible, spend less time optimizing, and make every one of your precious samples count with antibodies that perform.
Customize experiments with up to 4 lasers and 14 colors with options for up to 6 channels off of the violet laser. Get the freedom to build multicolor panels, accommodating common fluorophores and dyes.
Leverage a new platform that enables measurement of target-specific proteins such as IFNγ, IL-8, and IL-6. Invitrogen ProQuantum immunoassay kits utilize proximity ligation assay (PLA) technology and 2 μL of sample or less per run. Unlike other next-generation immunoassay platforms on the market, you do not need to buy an additional, closed system. ProQuantum immunoassays can be run on any existing qPCR instrument, which maximizes the value of the instruments you already have.
Invitrogen uncoated ELISA kits are ideal for experienced ELISA users on a budget, with less stringent requirements for inter- and intra-assay variance. These kits are several times more economical than ready-to-use ELISA kits that contain precoated plates.
Recover high percentages of nucleic acids from your samples and maximize your budget with TRIzol reagent for RNA and DNAzol reagent for genomic DNA. These organic reagents are well established. TRIzol has been references in over 70,000 publications. Extraction of RNA can be completed in under an hour. Genomic DNA extraction with DNAzol can be completed in 10-30 minutes with recoveries of 70-100%.
The Applied Biosystems SimpliAmp Thermal Cycler delivers high performance at an affordable price. The 96-well VeriFlex block has 3 independent temperature zones, for precise temperature control over your PCR optimization. The SimpliAmp Thermal Cycler also features an 8-inch color touch screen and is WiFi enabled for remote monitoring.
Backed by the proven reliability of Applied Biosystems technology, MiniAmp and MiniAmp Plus thermal cyclers were designed for routine PCR. With their compact design at just 7.5 inches (19 cm) wide and entry-level prices, these thermal cyclers are perfect for every lab bench.
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Benefit from a proven combination of Gibco cell culture media and Thermo Scientific Nunc plastics for consistent cell growth. Every lot of Nunc plastics with the Nunclon Delta cell culture–treated surface is verified with trusted Gibco media to confirm consistent cell growth across multiple cell lines. Only Thermo Scientific cell culture plastics endure rigorous testing with Gibco media, including cloning efficiency tests using specially selected cell lines with high sensitivity to toxic components. It’s the perfect pairing that can mean the difference between success and starting over.
We’re committed to helping you maximize every precious research sample. Feel reassured by tools that provide enhanced sensitivity so that you can accelerate your research path forward. From reagents to kits to instruments, our solutions are designed to empower your next discovery.
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Study up to 16-data parameters from your precious samples while saving time with the high-performance capabilities of the Invitrogen Attune NxT Flow Cytometer. This dynamic instrument is designed with active mechanical resistance to clogging, novel acoustics-assisted hydrodynamic focusing for speed, and sample acquisition from 96-well and 384-well microtiter plates for reliable automation. Immunophenotyping analysis on minimally processed samples can be performed using a simplified no-wash, no-lyse method, reducing the time-intensive traditional sample preparation protocols from 10 steps to 3 straightforward steps.
Invitrogen CytoVista Tissue Clearing Reagent sufficiently clears tissue for 3D fluorescent imaging, but unlike other clearing techniques does not significantly change the tissue morphology. Minimal morphological changes, such as shrinkage and contraction, have been observed. For precious samples, the clearing can be reversed, and the tissue can be processed for histology applications such as hematoxylin and eosin (H&E) staining.
The Invitrogen PureLink RNA Micro Scale Kit is designed to be used when input is limited. It provides rapid purification and concentration of total RNA from a variety of samples, including LCM tissues, fine-needle aspirates, and sorted cells.
Unlocking the information contained in FFPE samples is essential to cancer research. The Applied Biosystems MagMAX FFPE DNA/RNA Ultra Kit is designed for sequential isolation of DNA and RNA from the same FFPE tissue sample, with a minimal requirement of a 5 μm section or curl. The DNA and RNA are recovered in separate eluates, and both are compatible with a broad range of applications, including real-time PCR and NGS.
Invitrogen SYBR Gold Nucleic Acid Gel Stain is the most sensitive fluorescent stain we offer for nucleic acid detection. Maximize your precious DNA and RNA samples with enhanced sensitivity to detect as little as 25 pg of sample.
Invitrogen Platinum SuperFi DNA Polymerase is a proofreading enzyme that combines superior fidelity with trusted Platinum hot-start technology. Featuring >100x the fidelity of Taq polymerase, Platinum SuperFi DNA Polymerase is ideally suited for applications that benefit from exceptional sequence accuracy. In addition, the extreme robustness of the polymerase enables successful amplification of samples of suboptimal purity.
With simple cartridge-loaded reagents and a straight-forward user interface, the Ion GeneStudio S5 System makes NGS fast and easy—ideal for any cancer research lab. Minimize sample input and maximize flexibility. Ion AmpliSeq technology enables successful library preparation from challenging sample types such as formalin-fixed, paraffin-embedded (FFPE) tissue, retrospective samples from fine-needle aspirates, and cell-free DNA (cfDNA) extracted from blood, using as little as 1 ng of input DNA or RNA to support multiple applications. With this system, you can efficiently implement small and large projects across multiple research applications with a simple sample-to-data NGS workflow.
Applied Biosystems TaqMan Advanced miRNA assays enable highly sensitive and specific quantitation of mature miRNAs using qPCR. Together with the TaqMan Advanced miRNA cDNA Synthesis Kit, this solution provides a streamlined workflow that allows for the detection and quantitation of mature miRNA from as little as 1 pg of total RNA or 2 μL of plasma or serum.
Invitrogen SuperScript IV Reverse Transcriptase offers superior cDNA synthesis in a short 10-minute reaction. It is designed to provide reliable and consistent results even with the most challenging RNA samples.
The Ion Torrent Oncomine TCR Beta-LR Assay is designed to efficiently capture all three complementarity determining regions of the TCR beta chain gene (CDR1, CDR2, CDR3) with as little as 10ng of RNA input. This assay enables key applications such as predictive or prognostic biomarker discovery, T cell characterization, and identification of variable gene polymorphisms from samples such as RNA extracted from whole blood, fresh-frozen tissue, or sorted cells. The use of RNA template allows sequencing of productive and relevant variable (V), diversity (D), and joining (J) rearrangements—improving the identification of rare clones.
With a 3-day workflow using as little as 20 ng of tumor DNA, the Ion Torrent Oncomine Tumor Mutation Load Assay covers 1.65 Mb across 409 oncogenes relevant to major cancer types. The assay highly correlates with exome mutation counts and obviates the need for whole-exome sequencing, allowing for a higher percentage of samples to be evaluated and conserving precious samples for additional biomarker assessment. Additionally, our Oncomine informatics enable simultaneous assessment of TMB and variant profiling for greater actionable insights.
The Applied Biosystems Arcturus Cellect LCM System is optimized for applications ranging from single-cell isolation to extraction of small cell populations with a high degree of precision and flexibility. The instrument enables researchers to uncover unique molecular signatures that may be obscured in heterogeneous tumor samples. This system features the unique combination of a gentle infrared (IR) laser to capture the cells of interest and a powerful ultraviolet (UV) laser for efficient microdissection.
Applied Biosystems TaqMan Mutation Detection assays are powered by allele-specific TaqMan PCR (castPCR) technology to detect and measure somatic mutations in genes of interest to cancer research. This highly specific and sensitive technology can detect rare copies of mutated DNA in a sample that contains large amounts of wild type DNA—down to 1 cancer cell in 1,000 normal cells. These assays are compatible with sample types such as cell lines, FFPE tissue, and fresh-frozen tissue.
The Invitrogen Qubit Fluorometer is designed to quickly and specifically quantitate DNA or RNA. In addition, with the newest version of the Qubit Fluorometer, you can now easily measure RNA integrity and quality. It is now easier than ever to make sure you have enough DNA or RNA for your experiment. Use as little as 1 μL of sample, even with very dilute samples. Dyes in Invitrogen Qubit assays bind selectively to DNA, RNA, or protein, making them more sensitive than UV absorbance.
When working with challenging RNA samples or analyzing low-abundance transcripts, the ability of a reverse transcriptase to generate cDNA from the lowest amount of input RNA is crucial. Invitrogen SuperScript IV VILO Master Mix offers highly sensitive and robust cDNA synthesis for RT-qPCR applications in a short 10-minute reaction. Obtain higher cDNA yields and earlier Ct values (up to 6 cycles ahead of other reverse transcriptases), all while maintaining superior linearity across a broad range of RNA inputs. Additionally, superior inhibitor resistance allows for the utmost confidence in results, even with degraded or inhibitor-containing RNA samples.
Requiring low sample input, the Ion Torrent Oncomine TCR Beta-SR Assay specifically interrogates the CDR3 region of the TCR beta chain gene. Compatible with both FFPE DNA and RNA, this short-read sequencing assay enables characterization of immune status and detection of T cell clones in peripheral blood. The assay offers a 2-day turnaround time and is complete with superior informatics for accurate clonality and sequence assessment of the CDR3 region without interference from primer bias.
Clariom D Pico and Clariom S Pico microarray assays
Many expression profiling techniques require large amounts of input RNA, which are often not available in cancer research samples. Applied Biosystems Clariom Pico assays can generate detailed, robust, and accurate data from as little as 100 pg of total RNA (as few as 10 cells) from a wide range of challenging clinical research samples.
Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultrasensitive enhanced chemiluminescent (ECL) substrate for low-femtogram detection of protein by western blot analysis with the enzyme horseradish peroxidase (HRP). This substrate produces a quantitative signal that is easily captured by exposure to film or with an imaging system. The substrate offers excellent signal duration—8 hours of usable light output when conditions are optimized.
Invitrogen Bolt Bis-Tris Plus mini gels are designed for optimal separation of a broad molecular weight range of proteins under denaturing conditions during gel electrophoresis. The unique WedgeWell design allows loading of up to two times more sample volume than other precast gels. Bolt gels are ideal for western blot analysis and applications where protein integrity is crucial and higher sensitivity is required.
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Gibco extracellular matrix (ECM) products are essential tools for the growth, differentiation, and maturation of 2D and 3D cellular models. Products including Gibco Geltrex matrix, collagen, and the AlgiMatrix system are widely used to provide cells with more physiologically relevant environments that promote in vivo–like morphology. Lot-to-lot consistency and functional testing help provide increased confidence for specialized cell types like stem cells or cancer cell lines.
Techniques for gene manipulation, expression analysis, and cancer cell modeling can be difficult to implement in the lab. New technologies can help simplify these techniques and make them accessible to all researchers. This increased accessibility leads to broader adoption by the research community, allowing the techniques to be standardized over time. From nucleic acids to proteins to cells, empower your cancer research workflow.
The Thermo Scientific KingFisher Flex Purification System provides plate-based handling (mix, capture, transfer, release) of magnetic particles for bead-based nucleic acid and protein affinity purification procedures. This easy-to-use instrument provides excellent reproducibility and minimizes the hands-on time required for DNA, RNA, and protein isolation from a wide range of sample types and volumes.
Liquid biopsy is a noninvasive technique for molecular biomarker discovery that is expected to eliminate the need for costly, invasive procedures. Our wet lab–verified Applied Biosystems TaqMan Liquid Biopsy dPCR assays enable the sensitive detection and quantitation of low-frequency mutations, including the most common cancer-related mutations in EGFR, BRAF, KRAS, PIK3CA, JAK2, and other genes.
Invitrogen QuantiGene RNA assays are hybridization-based assays that utilize branched DNA signal amplification technology to measure gene expression. QuantiGene assays work directly with cell or blood lysates or tissue homogenates without RNA purification or enzymatic manipulation. Invitrogen QuantiGene Plex DNA CNV assays are also available to measure differences in copy number.
The Invitrogen PrimeFlow RNA Assay expands the capability of flow cytometry to measure RNA. It is the only known commercial assay that enables the simultaneous detection of up to four RNA targets in combination with immunophenotyping for cell-surface and intracellular proteins using fluorophore conjugated antibodies. The PrimeFlow RNA Assay can detect mRNA, lncRNA, and microRNA, as well as viral RNA and telomere DNA.
The Ion Torrent Oncomine Focus Assay is a targeted, multibiomarker assay that enables you to target hotspots, SNVs, indels, CNVs, and gene fusions in DNA and RNA in a single workflow. Designed for clinical and translational cancer research, the Oncomine Focus Assay leverages Ion AmpliSeq technology producing robust results, low input amounts, and analysis of low-quality samples, and is compatible with FFPE fine-needle aspirates and core-needle biopsies.
Current methods for the analysis of hematological malignancies often involve multiple sequential tests and laborious workflows. The Ion Torrent Oncomine Myeloid Research Assay is a comprehensive, targeted NGS assay designed to assist in the understanding of myeloid cancer in a single test. It interrogates relevant DNA mutations and fusion transcripts associated with myeloid disorders in a quick and easy NGS assay.
The Invitrogen Collibri library preparation kits and accessories generate libraries from many sample types including FFPE. Libraries are prepared more quickly, with less bias. Collibri library prep kits have incorporated the following features: visual feedback for higher library prep success, and shorter total workflow times.
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As you move from basic cell therapy research to the clinic, high-quality products and proper documentation are essential to getting it right the first time. Cell Therapy Systems (CTS) products help minimize the risk of contamination and variability in your research and provide the required documentation for regulatory review.
In cancer research, there is simply no room for error. Getting robust and reliable data from a single sample is critical to advancing your cancer research studies. Simple and efficient ways to share data can accelerate productivity and enhance collaboration. Learn how cloud-enabled tools are helping cancer researchers achieve better productivity, improved data, and streamlined data sharing.
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The Thermo Scientific CellInsight CX7 LZR High Content Analysis Platform is a fast, laser-based, automated cellular imaging and analysis platform for quantitative microscopy and phenotypic classification. It’s designed to provide you with the sensitivity and speed that’s needed for emerging assays. HCS Studio Cell Analysis Software offers intuitive interfaces and guided workflows for integrated data collection and analysis on CellInsight platforms.
The unique design of the Invitrogen Attune NxT Flow Cytometer offers progressive application capabilities, from 1 to 4 lasers and from 4 to 14 colors, providing expanded assay development flexibility and increasing the information you can obtain from your sample. This translates into more data with better population identification than if you were using fewer parameters.
The Applied Biosystems CytoScan HD Suite replaces the need to run multiple tests on multiple technologies to obtain comprehensive cytogenetic analysis of hematological malignancy samples. With the Cytoscan HD Suite solution you can detect chromosomal arm aberrations, focal changes, loss of heterozygosity (LOH), copy-neutral loss of heterozygosity (cnLOH), ploidy changes, chromothripsis, and more.
Get more RNA expression data from a single assay and expand your potential to discover novel, informative cancer biomarkers with Applied Biosystems Clariom D assays. Our novel microarray-based Clariom D Pico assays provide intricate transcriptome-wide gene and exon-level expression profiles, including the ability to detect alternative splicing events of coding RNA and long noncoding RNA (lncRNA) in a single 3-day experiment.
Minimize the barriers of next-generation sequencing (NGS) data analysis and simplify your bioinformatics path to discovery. Ion Reporter Software provides an optimized suite of simple tools that streamline data analysis for Ion GeneStudio S5, Ion PGM, and Ion Proton systems. With these tools, you can focus on finding the biological meaning of your data, share your findings with other researchers safely, and spend less time on configuring the software.
The types of genetic aberrations associated with childhood malignancies differ from those affecting the adult population, so a specifically designed, targeted but comprehensive panel is needed to advance clinical research and drive future therapeutic solutions for precision oncology. The Ion Torrent Oncomine Childhood Cancer Research Assay is a unique, NGS-based tool designed for comprehensive genomic profiling of cancers affecting children and young adults.
Reliably detect differences in gene expression as small as 1.5 fold on the Applied Biosystems QuantStudio 6 and 7 Pro real-time PCR systems. Multiplexing of 5 or 6 optical channels is available to gain more results from your most precious samples.
Monitor, analyze and share data from wherever you are using our connect cloud-cloud based platform and app. These systems are compatible with automation and TaqMan array plates with RFID to increase convenience and reduce manual errors.
See more copy number changes in your cancer research samples with the Applied Biosystems OncoScan CNV Assay. Designed to cover the whole genome as well as known cancer-driver events, this single microarray-based assay easily detects copy number gains and losses, LOH, cnLOH, ploidy, allele-specific changes, break points, mosaicism, clonal heterogeneity, and chromothripsis.
The Ion Torrent Oncomine Comprehensive Assay v3 is a member of the family of widely adopted Oncomine assays for clinical cancer research. Oncomine assays are NGS-based multi-biomarker assays used to profile thousands of samples in different translational and clinical research projects, and have consistently delivered reliable results.
The Ion Torrent Oncomine Immune Response Research Assay is a targeted NGS gene expression assay that enables immunotherapy research from the quantitative evaluation of the expression of markers associated with different leukocyte subsets, antigen presentation, checkpoint pathways, and tumor progression. This 400-gene assay is supported with a user-friendly informatics workflow and demonstrates high sensitivity for low-expressing transcripts derived from FFPE samples.
The Ion Torrent Oncomine BRCA Research Assay for the detection of BRCA somatic and germline mutations in formalin-fixed, paraffin-embedded (FFPE) tissue and whole blood has been widely adopted in molecular pathology research laboratories across the world. The assay can detect large insertions and deletions (indels) and exon or whole-gene deletion or duplication events, uniquely empowering laboratories to detect all classes of mutations in one NGS workflow.
Capture images and analyze data from your western blots and gels efficiently and easily using Invitrogen iBright imaging systems. These high-performance instruments enhance the imaging experience through advanced automated features and an interface that is easy to use for researchers of all experience levels.
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Invitrogen CytoVista 3D Cell Culture Clearing Reagent clears fluorescently labeled 3D cultured cells such as organoids and spheroids. This clearing process helps improve data quality by enabling sharp and bright 3D fluorescent imaging for depths up to 1,000 μm. The reagent can also be used as an imaging solution, with stable storage of 3D cell cultures for weeks with minimal change in tissue morphology, or brightness or specificity of the fluorophores.
Together, we can be smarter, faster, and more determined than ever in the fight against cancer. Collaboration is the key to efficiency. We strive to bring the research community together through impactful initiatives, virtual conferences, and live events. The collaborative spirit has evolved to become stronger and even more connected. Discover how we are helping researchers stay connected.
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Experimental reproducibility is vital for the advancement of cancer research. However, the replication of studies has long been a challenge due to the lack of characterization standards for antibodies (Figure 27). Everyone from the scientific community plays a role in this challenge: suppliers, researchers, and publishers. We can increase reproducibility by collaborating in three important ways:
- Research—verify your antibody and your experimental protocol
- Publish—use and list your controls and standards along with details about antibodies and experimental methods
- Train—expand your knowledge about experimental methods and controls, sample preparation, and other aspects of properly using antibodies
Thermo Fisher Scientific has implemented methods for the verification and reporting of antibody specificity to further enhance data transparency. These specificity tests help ensure that the antibody will bind to the correct target.
Reliably detect differences in gene expression as small as 1.5 fold on the Applied Biosystems QuantStudio 6 and 7 Pro real-time PCR systems. Multiplexing of 5 or 6 optical channels is available to gain more results from your most precious samples.
Monitor, analyze and share data from wherever you are using our connect cloud-cloud based platform and app. These systems are compatible with automation and TaqMan array plates with RFID to increase convenience and reduce manual errors.
Community empowerment for mass spectrometry (MS) users comes in the form of the Thermo Scientific Tandem Mass Tag (TMT) Research Award and the ProteomicsNOW MS Virtual Event. For research users of Thermo Scientific TMT labeling reagents (including amine-, sulfhydryl-, or carbonyl-reactive reagents), you could receive one of three awards. Terms & conditions
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Gibco Cell Culture Heroes spotlights PhD students and postdoctoral scientists across the globe with the primary focus of telling the story of their cancer research. This program promotes education and creates a growing community of researchers who are driving tomorrow’s breakthroughs in the fight against cancer.
For Research Use Only. Not for use in diagnostic procedures.