Figure 1. Qubit 4 Fluorometer workflow.
Fast and sensitive quantification of DNA, RNA, and Protein
The Invitrogen Qubit 4 Fluorometer is the next generation of the popular benchtop fluorometer designed to accurately measure DNA, RNA, and protein quantity. The new Qubit 4 also easily measures RNA integrity and quality. The easy-to-use touch screen menus make it easy to select and run the assays you need, with results displayed in just a few seconds.
Key features of the Qubit 4 Fluorometer include:
- More sensitive than UV absorbance–based quantification, making it ideal for precious samples
- Accurately quantifies DNA, RNA, and protein in < 3 seconds per sample
- Fast, reliable detection of degraded RNA with the new RNA IQ assay
- New integrated reagent calculator reports amount of dye and buffer needed
- Uses as little as 1 μL of sample
- Flexible options for exporting results: Wi-Fi, USB drive, or direct connection with a USB cable
The latest version of the Qubit fluorometer has been re-engineered to run the Qubit RNA IQ (integrity & quality) assay. The concentration or quality of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound to the target, which minimizes the effects of contaminants on the result.
How the Qubit Fluorometer works
Single-sample measurements are easy
This user-friendly fluorometer delivers results using quick, simple assay procedures. The large, color touch screen allows you to easily navigate through the various options. Calculations and settings are automatically performed by the instrument (Figure 1). The Qubit 4 Fluorometer will save the data for up to 1,000 samples, and you can transfer data using Wi-Fi, a USB drive, or direct connection with a USB cable. In addition, new Qubit 4 includes a built-in reagent calculator to easily determine the amount of dye and buffer required to prepare your samples and standards (Figure 2).
Figure 2. Integrated reagent calculator. Enter the number of samples and standards needed to calculate the amount of dye and buffer.
Assays for use with the Qubit Fluorometer
The Qubit 4 Fluorometer and all Qubit assays are optimized for use together. Qubit assays are highly sensitive and selective for DNA, RNA or protein. These assays are performed using the same basic protocol: simply mix and read, with incubation times of only 2 minutes required for DNA and RNA assays or 15 minutes for the protein assay. Qubit Assays cover a broad dynamic range for any starting sample concentration (Figure 3). In addition to our core assays for dsDNA, oligos, total RNA, microRNA, and protein, Ion Sphere Particle quality can be evaluated on the Qubit 4 Fluorometer using the Ion Sphere Quality Control Kit prior to performing a sequencing run on the Ion Personal Genome Machine (PGM) Sequencer.
Figure 3. Comparison of sample concentration ranges for Qubit assays and UV absorbance measurements. UV absorbance readings are not selective for RNA vs DNA. The Qubit 4 Fluorometer and all Qubit assays are optimized for use together.
Sensitive, accurate biomolecule quantification
The Qubit 4 Fluorometer detects fluorescent dyes that bind specifically to the target of interest. This target specific enables precise, accurate measurements even for dilute samples requiring high sensitivity.
Qubit Fluorometer product guide
| Qubit 4 Fluorometer | Qubit 4 Quantification Starter Kit | Qubit 4 NGS Starter Kit | Qubit 4 RNA IQ Starter Kit |
|---|---|---|---|
.jpg) |  |  |  |
|
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|
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| Q33241 |
Usually measuring more than a handful of samples?
The Invitrogen Qubit Flex Fluorometer could be a better solution for you if you are measuring eight samples or more at once. See if you can benefit from the time savings.
Featured video: Nucleic Acid Quantification—Comparing Qubit fluorometer to the Nanodrop One UV/Vis absorbance
The best of both worlds
The combination of the Thermo Scientific NanoDrop One UV-Vis Spectrophotometer and the Qubit Fluorometer enables accurate, easy, and fast quantification of biomolecules.
System specifications
| Instrument dimensions | 5.4 in (w) x 10 in (l) x 2.2 in (h) (13.6 cm x 25 cm x 5.5 cm) |
|---|---|
| Weight | 743 g |
| Dynamic range | 5 orders of magnitude |
| Processing time | ≤5 seconds/sample |
| Light sources | Blue LED (max ~470 nm) Red LED (max ~635 nm) |
| Excitation filters | Blue 430–495 nm Red 600–645 nm |
| Emission filters | Green 510–580 nm Red 665–720 nm |
| Detectors | Photodiodes: measurement capability from 300–1,000 nm |
| Warm-up time | <35 seconds |
| USB drive | 4 GB |
Fluorometer mode
By switching to Fluorometer mode, the Qubit Fluorometer can be used as a mini-fluorometer. In Fluorometer mode, rather than performing an assay calibration and calculating results based on an algorithm, the instrument generates and displays raw fluorescence (RFU) values for each sample.
Fluorometer mode allows you to manually select the excitation source: blue LED or red LED. If blue excitation is chosen, the instrument reads the fluorescence in both the green and far red emission channels; if red excitation is chosen, emission is read in the far-red channel only.
Data gathered in Fluorometer mode may be used to design your own assay. See MyQubit for more information.
Step 1. Access Fluorometer mode. To access Fluorometer mode, press the Fluorometer button from the home screen.
Step 2. Choose excitation Choose Blue (470 nm) or Red (635 nm) excitation from the menu.
Step 3. Insert sample. Insert your sample and press Read tube.
Step 4. Read tube For Blue excitation, the Qubit Fluorometer displays both the green emission and the far-red emission of your sample. For Red excitation, you will see the far-red emission of your sample. Remove the tube and press Read tube to read the next sample.
MyQubit—Envision and create new assays for the Qubit 4 Fluorometer
The Qubit 4 Fluorometer is compatible with the MyQubit assay design tool. MyQubit allows you to create new assays for the Qubit 4 Fluorometer in minutes. Simply enter your assay parameters into the online tool, and then save and upload the .qbt file to the Qubit 4 Fluorometer.
Invitrogen Qubit 4 Fluorometer: Quantitation
Invitrogen Qubit 4 Fluorometer: Managing Data
Invitrogen Qubit 4 Fluorometer: Additional Features
Invitrogen Qubit 4 and RNA Quantitation and Qualification
Quantitation: Comparing Qubit fluorometer to the Nanodrop One UV/Vis absorbance
Qubit Fluorometer product guide
| Qubit 4 Fluorometer | Qubit 4 Quantification Starter Kit | Qubit 4 NGS Starter Kit | Qubit 4 RNA IQ Starter Kit |
|---|---|---|---|
| | | |
|
|
|
|
| Q33241 |
Usually measuring more than a handful of samples?
The Invitrogen Qubit Flex Fluorometer could be a better solution for you if you are measuring eight samples or more at once. See if you can benefit from the time savings.
Featured video: Nucleic Acid Quantification—Comparing Qubit fluorometer to the Nanodrop One UV/Vis absorbance
The best of both worlds
The combination of the Thermo Scientific NanoDrop One UV-Vis Spectrophotometer and the Qubit Fluorometer enables accurate, easy, and fast quantification of biomolecules.
System specifications
| Instrument dimensions | 5.4 in (w) x 10 in (l) x 2.2 in (h) (13.6 cm x 25 cm x 5.5 cm) |
|---|---|
| Weight | 743 g |
| Dynamic range | 5 orders of magnitude |
| Processing time | ≤5 seconds/sample |
| Light sources | Blue LED (max ~470 nm) Red LED (max ~635 nm) |
| Excitation filters | Blue 430–495 nm Red 600–645 nm |
| Emission filters | Green 510–580 nm Red 665–720 nm |
| Detectors | Photodiodes: measurement capability from 300–1,000 nm |
| Warm-up time | <35 seconds |
| USB drive | 4 GB |
Fluorometer mode
By switching to Fluorometer mode, the Qubit Fluorometer can be used as a mini-fluorometer. In Fluorometer mode, rather than performing an assay calibration and calculating results based on an algorithm, the instrument generates and displays raw fluorescence (RFU) values for each sample.
Fluorometer mode allows you to manually select the excitation source: blue LED or red LED. If blue excitation is chosen, the instrument reads the fluorescence in both the green and far red emission channels; if red excitation is chosen, emission is read in the far-red channel only.
Data gathered in Fluorometer mode may be used to design your own assay. See MyQubit for more information.
Step 1. Access Fluorometer mode. To access Fluorometer mode, press the Fluorometer button from the home screen.
Step 2. Choose excitation Choose Blue (470 nm) or Red (635 nm) excitation from the menu.
Step 3. Insert sample. Insert your sample and press Read tube.
Step 4. Read tube For Blue excitation, the Qubit Fluorometer displays both the green emission and the far-red emission of your sample. For Red excitation, you will see the far-red emission of your sample. Remove the tube and press Read tube to read the next sample.
MyQubit—Envision and create new assays for the Qubit 4 Fluorometer
The Qubit 4 Fluorometer is compatible with the MyQubit assay design tool. MyQubit allows you to create new assays for the Qubit 4 Fluorometer in minutes. Simply enter your assay parameters into the online tool, and then save and upload the .qbt file to the Qubit 4 Fluorometer.
Invitrogen Qubit 4 Fluorometer: Quantitation
Invitrogen Qubit 4 Fluorometer: Managing Data
Invitrogen Qubit 4 Fluorometer: Additional Features
Invitrogen Qubit 4 and RNA Quantitation and Qualification
Quantitation: Comparing Qubit fluorometer to the Nanodrop One UV/Vis absorbance
Looking for Qubit assays?
See the full selection of Invitrogen Qubit assays to accurately detect DNA, RNA and protein using Qubit fluorometers.
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For Research Use Only. Not for use in diagnostic procedures.