Table 15.5 Fluorogenic substrates for caspase activity.
| Peptide Sequence | Target for Caspases * | Fluorophore † | |
|---|---|---|---|
| R110 | AMC | ||
| YVAD | 1, 4 | R33750 | |
| VDVAD | 2 | R33755 | |
| DEVD | 3, 6, 7, 8, 10 | E13184 ‡ R22120 § R35100 ** | E13183 ‡ |
| VEID | 6 | R33754 | |
| IETD | 8, granzyme B | R22125 R22126 § | |
| LEED | 13 | R33753 | |
| AAD | granzyme B | R33752 | |
| Aspartic acid | generic | R22122 | |
| * Caspase substrates can often be cleaved by multiple enzymes. The caspase most often associated with the given peptide sequence is listed first. † R110 = rhodamine 110; AMC = 7-amino-4-methylcoumarin. The absorption and emission maxima for the cleaved fluorophores at pH 7 are: 496/520 nm for R110 and 342/441 nm for AMC. ‡ EnzChek Caspase-3 Assay Kit. § Bulk packaging. ** RediPlate 96 EnzChek Caspase-3 Assay Kit. | |||
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