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Invitrogen Alexa Fluor protein and antibody conjugates produce fluorescence outputs that exceed those of other spectrally similar fluorophore conjugates and do not photobleach as easily as classic fluorescent secondary antibodies. With more than 80,000 publications, our Alexa Fluor secondary antibodies are reliable research tools. Browse our comprehensive portfolio of Alexa Fluor and Alexa Fluor Plus secondary antibodies for fluorescent detection of primary antibodies in a wide range of applications, such as cell imaging, flow cytometry, and western blotting.
Alexa Fluor secondary antibody product information
Alexa Fluor secondary antibodies have been tested extensively to help ensure optimal performance. Alexa Fluor Plus secondary antibodies have been optimized further to enable better detection of low-abundance targets and are excellent for use in rare or precious samples.
| Alexa Fluor Secondary Antibodies | Alexa Fluor Plus Secondary Antibodies | |
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| Use | Designed for use in a variety of cell analysis and protein analysis applications to detect targets at varying expression levels |
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| Targets species available |
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| Fluorophores available | 18 Alexa Fluor dyes available | 7 Alexa Fluor Plus dyes available |
| Antibody formats available |
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| Mountant compatibility | Most standard fixatives. See the selection guide: Mounting Media and Antifades Recommended: | |
Alexa Fluor and Alexa Fluor Plus selection guide
Alexa Fluor secondary antibody formats
Our Alexa Fluor and Alexa Fluor Plus conjugated secondary antibodies are now available in a variety of formats, each offering unique benefits.
F(ab) and F(ab’)2 fragment Alexa Fluor secondary antibodies
We offer F(ab) and F(ab’)2 fragment secondary antibodies with various Alexa Fluor conjugate options. These secondary antibody fragments are cross-adsorbed to increase sensitivity, reduce background staining, and offer several benefits over whole IgG antibodies. Because of their smaller size they can more easily penetrate complex tissue models compared to whole IgG molecules, potentially leading to more antigen binding capability. This property, combined with Alexa Fluor and Alexa Fluor Plus conjugation, makes these molecules valuable staining tools in immunohistochemistry and immunofluorescent experiments.
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Comparison of whole IgG with F(ab’)2 Alexa Fluor Plus 555 secondary antibody in human small intestine (duodenum). (A) Cell junctions were stained using Cadherin-17 Monoclonal Antibody (3B10A9) (Cat No. 60351-1-IG) and Alexa Fluor Plus 555 whole IgG secondary antibody. (B) Under matched conditions, cell junctions were stained using the same primary antibody against Cadherin 17 and F(ab')2-Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555 (Cat. No. A48287). Nuclei were stained with Hoechst 33342 (Cat. No. H3570). Images were captured on an EVOS M7000 instrument at 4x magnification.
Superclonal recombinant Alexa Fluor secondary antibodies
Superclonal recombinant secondary antibodies provide polyclonal sensitivity with monoclonal specificity in a recombinant format. Now available with a variety of Alexa Fluor and Alexa Fluor Plus conjugates, our Superclonal recombinant secondary antibodies help ensure reliable results with their lot-to-lot consistency.
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Superclonal secondary antibodies are designed to provide lot-to-lot consistency. Endogenous TOMM20 in HeLa cells were labeled with rabbit TOMM20 monoclonal primary antibody, which was then detected with four different lots of Goat anti-Rabbit IgG (Heavy Chain), Superclonal Recombinant Secondary Antibody, Alexa Fluor Plus 488 (Cat. No. A55053) (green). Comparable signal and noise fluorescence intensities from these four lots demonstrate lot-to-lot consistency of Superclonal secondary antibodies. Inset represents control cells with no primary (NP) antibody to assess noise.
Alexa Fluor ReadyProbes Secondary Antibodies
ReadyProbes secondary antibodies are conjugated with bright and photostable Alexa Fluor 488 and Alexa Fluor 594 dyes to offer convenient and robust labeling. These Alexa Fluor secondary antibodies recognize IgG heavy chains and all classes of immunoglobulin light chains from either mouse or rabbit. Like other ReadyProbes reagents, these secondary antibodies are provided in a convenient dropper bottle format. No pipetting or dilutions are required.
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Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody in ICC/IF. CAKI cells were plated on coverslips overnight. Then, cells were fixed and permeabilized. Following suppression of background binding using Image-iT FX Signal enhancer (Cat. No. R37107), peroxisomes were labeled using a rabbit anti-PMP70 antibody followed by detection with Goat anti-Rabbit IgG (H+L) Cross-Adsorbed ReadyProbes Secondary Antibody, Alexa Fluor 488 (Cat. No. R37116). Cells were washed three times in dPBS and nuclei were stained with NucBlue Fixed Cell ReadyProbes Reagent (DAPI) (Cat. No. R37606) and actin was visualized with ActinRed 555 ReadyProbes Reagent (Rhodamine phalloidin) (Cat. No. R37112). Images were acquired on the EVOS FL Auto imaging system using a 40x coverslip-corrected objective.
Alexa Fluor secondary antibody resources
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For Research Use Only. Not for use in diagnostic procedures.