Flow cytometric analysis of Jurkat cells labeled with Annexin V conjugate. Jurkat cells were induced with 10 µM camptothecin. Cells were washed and stained with Annexin V, Alexa Fluor 680 conjugate, and SYTOX Green Nucleic Acid Stain. These cells were analyzed on flow cytometer with 488 and 633 nm excitation. Emission Filter 515/30 and 660/20nm BP.
 |  |  |  |  |  |  |
| 3 | 633 | Cy5.5 | 681 | 704 | (in buffer) 1 (in antifade) 4 | microscopy; flow cytometry; fluorescence microplate reader |
Invitrogen Alexa Fluor 680 dye is a bright, near-infrared fluorescent dye with excitation well suited for the 633 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 680 dye is pH-insensitive over a wide molar range. In addition, it is used for multiplexing western blot detection and stochastic optical reconstruction microscopy (STORM) where it is used as a reporter for STORM.
The fluorescence quantum yield and high photostability in antifade allow detection of low-abundance biological structures in fixed cells with great sensitivity. Alexa Fluor 680 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. The long wavelength emission allows for detection in complex samples with auto-fluorescent background signals.
We offer Alexa Fluor 680 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.
Protein Labeling Reagents Selection Guide (NHS ester, maleimide, etc.) Search Alexa Fluor 680 secondary antibodies
Click image to enlarge
Fluorescence microscopy of mouse NIH 3T3 cells. NIH 3T3 mouse fibroblast cells were fixed and permeabilized. Golgi was labeled with green-fluorescent Lectin HPA, Alexa Fluor 488 Conjugate, mitochondria were labeled with a primary antibody directed against OxPhos Complex V Inhibitor Protein and visualized using far-red-fluorescent Goat anti-Mouse IgG Cross-Adsorbed Secondary Antibody, Alexa Fluor 680. Filamentous actin was stained with red-orange–fluorescent Alexa Fluor 568 Phalloidin. Nuclei were stained with blue-fluorescent DAPI.
Additional resources
Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.