Immunofluorescent analysis of caveolin 3 in C2C11 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a caveolin 3 polyclonal antibody (Cat. No. PA1-066) at a dilution of 1:20 overnight at 4°C, washed with PBS and incubated with a DyLight 488-conjugated secondary antibody (Cat. No. 35503). Caveolin 3 staining (green), F-actin staining with phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60x magnification.
Caveolae are small, specialized areas of the plasma membrane. These organelles are involved in sequestering a variety of protein and lipid molecules. Three caveolin proteins (caveolin-1, caveolin-2, and caveolin-3) are the primary components of caveolae, but their distribution varies with tissue type. Caveolins 1 and 2 have similar expression in fibroblasts, differentiated adipocytes, endothelial cells, and smooth and skeletal muscle. Caveolin-3 appears to be expressed only in muscle tissues. When internalized, caveolae can fuse with early endosomes, which then may be recycled back to the plasma membrane or sent on for degradation.
Caveolae are involved in many important biological functions including lipid metabolism, vesicular trafficking, signal transduction, endocytosis, exocytosis, cell adhesion, and apoptosis. They also play a role in neurodegenerative disease, oncogenesis, and the uptake of some viruses and pathogenic bacteria.
Caveolae marker antibodies can aid in the study of caveolae structure and function. Caveolae marker antibodies can also help elucidate the role or roles a protein may play in a number of tasks that are centered in or influenced by the caveolae. Invitrogen caveolae marker antibodies are designed to dependably detect the key caveolae targets. Each antibody is validated for use in various applications. To see all the caveolin antibody offering please click the links below.
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Quality Invitrogen caveolae marker antibodies are available for a variety of research needs.
Immunohistochemistry was performed on normal biopsies of deparaffinized Rat lymph node tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse Monoclonal Antibody recognizing Caveolin 1 (Cat. No. MA3-600) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.