Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
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| 4 | 633 | Cy7 | 752 | 776 | (in buffer) 1 (in antifade) 4 | microscopy, flow cytometry, fluorescence microplate reader |
Invitrogen Alexa Fluor 750 dye is a bright, near-infrared fluorescent dye with excitation well suited for the 633 nm laser line or dye-pumped laser excitation. For stable signal generation in imaging and flow cytometry, Alexa Fluor 750 dye is pH-insensitive over a wide molar range. Tandem conjugates of Alexa Fluor 750 on primary and secondary antibodies are widely used in flow cytometry.
Long-wavelength emission is excellent for imaging in tissues with background autofluorescence. Alexa Fluor 750 dye is particularly useful in stochastic optical reconstruction microscopy (STORM), where it is an exceptional reporter for both dSTORM and nSTORM.
We offer Alexa Fluor 750 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to help you generate your own antibody conjugates or probes.
Protein Labeling Reagents Selection Guide (NHS ester, maleimide, etc.) Search Alexa Fluor 750 secondary antibodies
Additional products
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Flow cytometry analysis of human lymphocytes using APC-Alexa Fluor 750 dye. Human peripheral blood lymphocytes were stained using CD8 Monoclonal Antibody (3B5), APC-Alexa Fluor 750. The negative control profiles represent unstained cells.
STORM imaging of immunolabeled microtubules. Tyrosinated tubulin was stained with Alexa Fluor 647 dye (magenta) and detyrosinated tubulin was stained with Alexa Fluor 750 dye (green). Square box represents zoomed region of antibody-labeled microtubules. Image courtesy of Joshua Vaughan et al., University of Washington, Seattle, WA.
Fluorescence microscopy of mouse kidney tissues using Alexa Fluor Plus 750 Phalloidin. Cryosectioned mouse kidney tissues were labeled with NucBlue Live ReadyProbes Reagent (Hoechst 33342) and Alexa Fluor Plus 750 Phalloidin . Labeled tissues were imaged using the EVOS Cell Imaging System with an Olympus 20X Apochromat objective, EVOS Light Cube, DAPI 2.0 and EVOS Light Cube, Cy7 2.0.
Additional resources
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
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