Table 5.1 Molecular Probes heterobifunctional crosslinkers.
| Cat # | Crosslinker |
Reactivity
| ||||
|---|---|---|---|---|---|---|
| Thiol (R–SH) | Amine (R–NH2) | Azide * (R–N3) | Alkyne * | Photoreactive † | ||
| S1553 | succinimidyl acetylthioacetate (SATA) ‡ | X | X | |||
| S1534 | succinimidyl trans-4-(maleimidylmethyl) cyclohexane-1-carboxylate (SMCC) ‡ | X | X | |||
| S1531 | succinimidyl 3-(2-pyridyldithio)propionate (SPDP) | X | X | |||
| E2247 | 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDAC) § | X | ||||
| P6317 | N-((2-pyridyldithio)ethyl)-4-azidosalicylamide (PEAS; AET) ‡ | X | X | |||
| A2522 | 4-azido-2,3,5,6-tetrafluorobenzoic acid, succinimidyl ester (ATFB, SE) | X | X | |||
| B1508 | benzophenone-4-maleimide | X | X | |||
| B1526 | benzophenone-4-isothiocyanate | X | X | |||
| B1577 | 4-benzoylbenzoic acid, succinimidyl ester | X | X | |||
| I10188 | iodoacetamide azide * | X | X | |||
| I10189 | iodoacetamide alkyne * | X | X | |||
| C10413 | Click-iT maleimide DIBO alkyne * | X | X | |||
| A10280 | azido (PEO)4 propionic acid, succinimidyl ester * | X | X | |||
| A10279 | alkyne, succinimidyl ester * | X | X | |||
| C10414 | Click-iT succinimidyl ester DIBO alkyne * | X | X | |||
| * Azides react with alkynes via the copper-catalyzed azide–alkyne cycloaddition reaction, as discussed in Click Chemistry—Section 3.1 (where these products are also described). † Reacts nonspecifically with available sites upon UV illumination. ‡ These reagents contain cryptic thiols that are exposed by disulfide reduction (SPDP or PEAS) or deacetylation (SATA) and can be subsequently disulfide-coupled to other thiolated molecules or thioether-coupled to maleimides or iodoacetamides. § EDAC couples amines to carboxylic acids (Derivatization Reagents for Carboxylic Acids and Carboxamides—Section 3.4). | ||||||
For Research Use Only. Not for use in diagnostic procedures.