Table 4.1 Biotinylation and desthiobiotinylation reagents.
| Reactive Moiety | Biotin Derivative (Cat. No.) | DSB-X Biotin Derivative (Cat. No.) |
|---|---|---|
| Aliphatic amine | A1593, A1594, B1596 (XX), N6356 | |
| Alkyne | B10185 | |
| Azide | B10184 | |
| Carboxylic acid | B1595, B20656, B1592 | D20657 |
| DNP-X–biocytin-X, SE | B2604, F6348 (F) | |
| Hydrazide | B1603, B2600 (XX) | D20653 (X) |
| Hydroxylamine | A10550 | |
| Iodoacetamide | B1591 | D30753 |
| Maleimide | M1602 | |
| Succinimidyl ester (SE) | B1513, B1582 (X), B1606 (XX), B6353 (SSE)(X), B6352 (SSE)(XX), A10495 (APEX), B30010 (Micro), B30756 (Micro), F2610 (F), F6347 (F) | D20655 (D) |
| TS-Link thiosulfate | T30754 | |
| (APEX) = APEX Antibody Labeling Kit. (D) = DSB-X Biotin Protein Labeling Kit. (F) = FluoReporter Protein Labeling Kit. (Micro) = Biotin-XX Microscale Protein Labeling Kit. (SE) = Succinimidyl ester. (SSE) = Sulfosuccinimidyl ester. (X) = Aminohexanoyl (7-atom) spacer separating the biotin or desthiobiotin and the reactive moiety. (XX) = Aminohexanoylaminohexanoyl (14-atom) spacer separating the biotin and the reactive moiety. | ||
For Research Use Only. Not for use in diagnostic procedures.