Bz-rA CEP
Designed for oligonucleotide synthesis for diagnostic and therapeutic development
Thermo Scientific RNA phosphoramidites are built to meet the varying needs of oligonucleotide manufacturers for the development of therapeutic, diagnostic, and research applications. We offer TheraPure, standard, and fast deprotect phosphoramidites.
- TheraPure phosphoramidites undergo additional quality control release testing and are compared with our standard phosphoramidites, helping to ensure that impurities and residual solvents are controlled to the stringent levels required by our customers for their oligo therapeutics.
- Standard phosphoramidites support the quality and reliability suitable for routine oligonucleotide synthesis.
- Fast deprotect phosphoramidites allow the removal of base protecting groups under mild or accelerated conditions without the need for special reagents.
Our RNA phosphoramidites are supported by:
- Expert technical support—over 40 years of partnership, customization, and scale-up experience
- Secure and consistent supply—5x capacity expansion in 2022
- Service excellence—48 hours or less response to orders on standard business days, >98% delivery to customer at promised date†
- Flexible packaging—custom and bulk packaging available upon request
- Quality—ISO 9001:2015–certified quality management system that has been refined in partnership with our customers since 1995
Quality manufacturing and regulatory documentation
Processes are monitored from material procurement through final QC and product release for batch-to-batch reproducibility. Purity of each phosphoramidite lot is assessed by HPLC-MS, GC, 31P NMR, as well as Karl Fischer oven titration. Our rigorously controlled manufacturing processes help minimize levels of reactive P(III) impurities which could be incorporated into the oligonucleotide during synthesis and result in the accumulation of by-products.
Available RNA phosphoramidites by synthesizer
Applied Biosystems/AKTA Synthesizer | Expedite Synthesizer | MerMade Synthesizer | Bulk | ||
---|---|---|---|---|---|
TheraPure phosphoramidites | Bz-rA | ✓ | ✓ | ✓ | ✓ |
Ac-rC | ✓ | ✓ | ✓ | ✓ | |
iBu-rG | ✓ | ✓ | ✓ | ✓ | |
rU | ✓ | ✓ | ✓ | ✓ | |
Standard phosphoramidites | Bz-rA | ✓ | |||
Ac-rC | ✓ | ||||
iBu-rG | ✓ | ||||
rU | ✓ | ||||
Fast deprotection (mild) | PAC-rA | ✓ | ✓ | ||
iPrPAC-rG | ✓ | ✓ | |||
Ac-rG | Contact us |
For items not listed above, flexible/bulk packaging, or custom services, please contact us ›
Product information
TheraPure RNA phosphoramidites were engineered specifically for oligonucleotide manufacturers requiring highly defined impurity profiles, strictly controlled processes with thorough documentation, and a rigorous process change control. With our in-house advanced analytical capabilities including LC-MS, GC, HPLC, 31P NMR and 1H NMR, meeting possible regulatory requirements should be achievable. Collaborative, customized specifications are always welcome for further discussion.
- QC testing always performed on raw materials and intermediates using high-definition analytics
- State-of-the-art purification methods utilized to obtain the highest amidite purity
- Overall, high purity and tight critical impurity level control yields excellent batch-to-batch reproducibility
Click images to enlarge
Bz-rA CEP | iBu-rG CEP | Ac-rC CEP | rU CEP | |
---|---|---|---|---|
27-1903 | 27-1906 | 27-1805 | 27-1804 | |
Formula | C53H66N7O8PSi | C50H68N7O9PSi | C47H64N5O9PSi | C45H61N4O9PSi |
Molecular weight | 988.2 g/mol | 970.1 g/mol | 902.1 g/mol | 861.1 g/mol |
CAS # | 104992-55-4 | 147201-04-5 | 121058-88-6 | 118362-03-1 |
Appearance | Color intensity must not exceed Pantone #P1-4U or #P4-2U | |||
Solution Clarity | Free from undissolved particulate at 0.2M solution in ACN | |||
HPLC purity | ≥99% | |||
31P NMR purity | ≥99% | |||
Residual Solvent | ≤3.0% total % by weight solvent residue | |||
Shipping / Storage condition | Ambient / –20°C | |||
Water content | ≤0.3% by KF Method | |||
Material Origin | Non-animal source (no TSE/BSE risk) | |||
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Use these additional amidites with Ac-rC and U to mitigate the time for your oligonucleotide deprotection.
Click images to enlarge
Therapure PAC-rA CEP | Therapure iPrPAC-rG CEP | |
---|---|---|
Cat. No. | 27-1803 | 27-1806 |
Formula | C54H68N7O9PSi | C57H74N7O10PSi |
Molecular weight | 1018.2 g/mol | 1076.3 g/mol |
CAS # | 121058-86-4 | 956580-73-7 |
Solution Clarity | 0.2M solution in ACN is free from undissolved particulate | |
HPLC purity | ≥99% | |
31P NMR purity | ≥99% | |
Water content | ≤0.3% (KF Method) | |
Shipping / Storage condition | Ambient / –20°C | |
Material Origin | Non-animal source (no TSE/BSE risk) | |
Request a quote |
Traditional base protection methods are used to ensure quality and reliability for routine oligonucleotide synthesis.
Click images to enlarge
Bz-rA CEP | iBu-rG CEP | Ac-rC CEP | rU CEP | |
---|---|---|---|---|
Cat. No. | 27-1403 | 27-1406 | 27-1405 | 27-1404 |
Formula | C53H66N7O8PSi | C50H68N7O9PSi | C47H64N5O9PSi | C45H61N4O9PSi |
Molecular weight | 988.2 g/mol | 970.1 g/mol | 902.1 g/mol | 861.1 g/mol |
CAS # | 104992-55-4 | 147201-04-5 | 121058-88-6 | 118362-03-1 |
Appearance | Color intensity must not exceed Pantone #P1-4U or #P4-2U | |||
HPLC purity | ≥98.0% | |||
31P NMR purity | ≥98.0% | |||
Shipping / Storage condition | Ambient / –20°C | |||
Water content | ≤0.3% by KF Method | |||
Material Origin | Non-animal source (no TSE/BSE risk) | |||
Request a quote |
RNA phosphoramidite ordering information
For Research Use Only. Not for use in diagnostic procedures.