Pierce Microdialysis Plates offer a ready-to-use disposable dialysis solution designed for multiplexing of many small volume samples, up to 96 x 0.1–0.3 mL samples (or 48 x 0.3–1.0 mL samples) in parallel with a variety of MWCOs. The devices feature regenerated cellulose membranes with a very large surface-area-to-volume ratio for dialysis experiments that take just 2–4 hours to complete, and provide greater than 90% protein recovery.
Pierce Microdialysis Plates are commonly used for a variety of protein sample cleanup purposes including protein and DNA desalting/buffer exchange, small molecule contaminates and unused reagent removal, and removal of dyes after protein labeling.
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| Plate size | 96-well | 96-well | 48-well |
| Max volume | 0.1 mL | 0.3 mL | 1 mL |
| See all Pierce Microdialysis plates | |||
96-well deep-well plate, 2.2 mL—polypropylene deep-well assay plate is required for 96-well microdialysis devices; for 48-well microdialysis kits, the deep-well plate is provided by default.
Plate seal—adhesive-coated plastic sheets are recommended to seal 96-well or 48-well microdialysis plates, to protect against contamination and evaporation, and are also suitable for long-term storage.
Each microdialysis plate includes 12 8-dialysis device strips which can be removed from the plate and separated into individual devices. This reduces waste allowing for only the number of dialysis devices needed. The 96-well microdialysis plate procedure involves simply removing the number of dialysis devices needed for your experiment, adding dialysis buffer to your deep well plate or microcentrifuge tube, loading your sample into the microdialysis device, placing the device with sample into the plate prepared with buffer, dialyzing, and recovering your sample with a pipet (Figure 1). Dialysis time will vary depending on salt and molecular weight concentrations.
Microdialysis devices are offered at 2K, 3.5K, 10K, and 20K MWCOs. Experimental data demonstrate that the rate of dialysis, as shown in conductivity (milliSiemens (mS)), is similar for the various 100 μL microdialysis device MWCOs (Figure 2). When evaluating efficiency of timed dialysis changes, conductivity decreases significantly in just 30 minutes, demonstrating that the majority of low molecular weight compounds are removed from the samples (Figure 3).
Figure 2. Similar rate of dialysis across different MWCOs—100 µL. Samples of 0.1 mL (0.5 mg/mL Human IgG containing 1 M NaCl) were dialyzed against 1.8 mL of water in a 96 deep-well plate at room temperature. The water was changed at 30 minute intervals over a 2.5 hour period. The rate of NaCl removal was determined by measuring the conductivity of the sample at the indicated time intervals.
Figure 3. Comparing of efficiency of timed dialysis changes—96 well. Samples of 0.1 mL (0.5 mg/mL Human IgG containing 1 M NaCl) were dialyzed against 1.8 mL of water in a 96 deep-well plate at room temperature. The water was changed at 15-, 30-, and 1 hour intervals over a 3 hour period. The rate of NaCl removal was determined by measuring the conductivity of the sample at the indicated time intervals.
Try Thermo Scientific Slide-A-Lyzer G3 Cassettes or Slide-A-Lyzer Dialysis Flasks for volumes from 1 mL to 250 mL. Slide-A-Lyzer dialysis cassettes and flasks offer a user-friendly approach to traditional dialysis tubing by incorporating dialysis membranes into a rigid frame with a secure cap which allows for easier sample loading/retrieval and increased sample security.
For Research Use Only. Not for use in diagnostic procedures.
