This section provides educational resources and tools for immunophenotyping by flow cytometry. These resources include a panel design tool, review articles, application notes, protocols, fluorophore guides and more.
Immunophenotyping features
Review article
Guidelines for the use of flow cytometry and cell sorting in immunological studies
This special edition of the European Journal of Immunology is a comprehensive resource prepared by flow cytometry and immunology research experts from around the world. The Flow Cytometry Guidelines not only highlight the latest trends and best practices in basic and advanced flow cytometry but also re‐enforce optimal sample preparation, as well as data analysis, enabling the correct use of state of the art flow cytometry for all involved in immunological research
Panel design tool
Flow Cytometry Panel Builder
This online tool guides you through flow cytometry panel design, providing a simplified, customizable experience to fit your flow cytometry panel design needs.
Immunophenotyping subtopics
Immunophenotyping learning resources
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| Type | Title | Categories |
|---|---|---|
| Application note (2017) | Validated 10-color T cell panel using the Attune NxT Flow Cytometer | antibodies, Attune/Attune NxT Flow Cytometer, flow cytometry, immunology, immunophenotyping, fluorescent dyes, multicolor flow cytometry |
| Application note (2017) | Detection of platelets in whole blood using the Attune NxT Flow Cytometer | Attune NxT, flow cytometer, platelets, immunophenotyping, no-wash, no-lyse |
| Application note (2015) | Attune NxT Flow Cytometer for 6-color immunophenotyping analysis of stained human whole blood using a no-lyse, no-wash protocol, with no compensation | Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | Detection of murine regulatory T cells on the Attune NxT Flow Cytometer | Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | Multiparameter analysis of murine regulatory T cells and dendritic cells with the Attune NxT Flow Cytometer | Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | Ten-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows identification of multiple T cell subsets and myeloid cells | Attune NxT, flow cytometer, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | 13-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows for the identification of B cells, NK cells, multiple T cell subsets and myeloid cells | Alexa Fluor, antibodies, Attune NxT, compensation, flow cytometer, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot |
| BioProbes articles (Issues 50– present day) | BioProbes Journal of Cell Biology Application | cell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification |
| Blog post | Super Bright conjugates for flow cytometry | antibodies, Attune NxT, flow cytometry, immunophenotyping, multicolor flow cytometry, Super Bright |
| Fluorophore | Pacific Blue dye | antibody conjugates, flow cytometry, immunophenotyping, violet laser |
| Fluorophore | Pacific Green dye | antibody conjugates, flow cytometry, immunophenotyping, violet laser |
| Fluorophore | Pacific Orange dye | antibody conjugates, flow cytometry, immunophenotyping, violet laser |
| Fluorophore | Super Bright 436 dye | antibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser |
| Fluorophore | Super Bright 600 dye | antibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser |
| Fluorophore | Super Bright 645 dye | antibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser |
| Fluorophore | Super Bright 702 dye | antibody conjugates, flow cytometry, immunophenotyping, tandem polymer dyes, violet laser |
| Fluorophore | Allophycocyanin (APC) | antibody conjugates, flow cytometry, immunophenotyping, phycobiliproteins, tandem dyes |
| Fluorophore | R-phycoerythrin (R-PE) | antibody conjugates, flow cytometry, immunophenotyping, phycobiliproteins, tandem dyes |
| Fluorophore | Cyan fluorescent protein (CFP) | flow cytometry, fluorescent proteins |
| Fluorophore | Green fluorescent protein (GFP) | flow cytometry, fluorescent proteins |
| Fluorophore | Red fluorescent protein (RFP) | flow cytometry, fluorescent proteins |
| Fluorophore | Fluorophore selection guide | antibody conjugates, flow cytometry, immunophenotyping |
| Guided learning | T cell stimulation and proliferation eLearning course | antibodies, cell proliferation, cell stimulation, flow cytometry, immunoassays, immunology, immunophenotyping, T cells |
| Molecular Probes Handbook | Phycobiliproteins—Section 6.4 | antibody labeling, multicolor flow cytometry, tandem conjugates |
| Molecular Probes Handbook | Using the Fluorescence SpectraViewer—Note 23.1 | flow cytometry, fluorescence, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins |
| Molecular Probes Handbook | Zenon Technology: Versatile reagents for immunolabeling—Section 7.3 | antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, secondary detection, Zenon |
| Immunology information | Foxp3 overview | flow cytometry, Foxp3,intracellular staining |
| Protocols | Cell preparation for flow cytometry protocols (Invitrogen eBioscience reagents) | flow cytometry, sample preparation |
| Protocols | Red blood cell lysis protocols using eBioscience lysis buffers (Invitrogen eBioscience reagents) | flow cytometry, sample preparation |
| Protocols | Staining cell surface targets for flow cytometry (Invitrogen eBioscience reagents) | extracellular staining, flow cytometry, immunophenotyping |
| Protocols | Staining intracellular antigens for flow cytometry (Invitrogen eBioscience reagents) | flow cytometry, immunophenotyping, intracellular staining |
| Protocols | Super Bright staining buffer protocol | antibody conjugates, flow cytometry, immunophenotyping |
| Scientific poster (2018) | Modular, defined assembly of antibody-based constructs using click chemistry | Alexa Fluor, antibody labeling, Attune/Attune NxT, CellInsight, drug discovery, drug research, high content analysis (HCA), SiteClick, spheroids |
| Scientific poster (2018) | Prospective cytometric study evaluating the alkaline phosphatase activity as a potential functional biomarker of primitive malignant cells in acute leukemia | antibodies, Attune/Attune NxT, cancer, cell function, flow cytometry, immunophenotyping, stem cell research |
| Scientific Poster (2017) | T-lymphocyte immunophenotyping: 14-color flow cytometry panel design using the Attune NxT Flow Cytometer and Super Bright fluorescent dyes | antibodies, Attune NxT, fluorescent dyes, immunophenotyping, multicolor flow cytometry, particles, Super Bright |
| Scientific Poster (2015) | A no lyse, no-wash approach to characterizing phagocyte phenotype and function in whole human blood on the Attune NxT Flow Cytometer | Attune NxT, cell cycle, flow cytometer, flow cytometry assays, immunophenotyping, no lyse, phagocytosis, whole blood |
| Scientific poster (2014) | Copper-safe Click-iT Plus EdU proliferation assay: Improved compatibility with simultaneous phycobiliprotein and fluorescent protein detection | cell proliferation, Click-iT, flow cytometry, immunophenotyping |
| Scientific poster (2013) | Improved click chemistry demonstrating EdU cell proliferation with GFP-expressing cells and R-PE-based immunophenotyping | Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometry assays, fluorescent dyes, fluorescent proteins, immunophenotyping |
| Scientific poster (2010) | Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry and imaging | Alexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF), immunophenotyping, stem cell research, violet laser-excited reagents |
| Scientific poster (2009) | A comparative study of 25 different CD4 conjugates | Alexa Fluor, antibodies, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot |
| Scientific poster (2009) | A novel xeno-free and serum-free culture medium for the expansion of human mesenchymal stem cells | cell culture, cell expansion, flow cytometry, fluorescent dyes, gene expression, immunophenotyping, stem cell research |
| Scientific poster (2009) | A simple, fast and quantitative single-step dead-cell indicator for flow cytometry | apoptosis, fluorescent dyes, multicolor flow cytometry, nucleic acid quantitation, viability |
| Scientific poster (2009) | Dead cell stains in flow cytometry: A comprehensive analysis | apoptosis, cells, flow cytometry, fluorescent dyes, immunophenotyping, viability |
| Scientific poster (2009) | Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry | Alexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescent dyes, fluorescent proteins, immunophenotyping, nucleic acid quantitation, stem cell research, violet laser-excited reagents |
| Scientific poster (2008) | Zenon labeling technology facilitates detection of intra-cellular phosphoprotein determinants | Alexa Fluor, antibodies, cell signaling, cells, flow cytometry, fluorescent dyes, protein labeling, rare cell detection, Zenon |
| Selection guide | Antibody fixation considerations | sample preparation |
| Selection guide | Human antibody cross reactivity chart | antibody cross-reactivity, flow cytometry |
| Selection guide | Mouse antibody cross reactivity chart | antibody cross-reactivity, flow cytometry |
| Selection guide | Cytokine bioassays guide | cytokines, flow cytometry, immunophenotyping, intracellular staining |
| Selection guide | Intracellular staining guide (for mouse & human cytokines and non-cytokine proteins) | cytokines, flow cytometry, intracellular staining |
| Selection guide | Human CD & other cellular antigens | flow cytometry, immunology, immunophenotyping, extracellular staining |
| Selection guide | Intracellular staining buffer selection guide | flow cytometry, immunophenotyping, intracellular staining |
| Selection guide | Mouse CD & other cellular antigens | flow cytometry, immunology, immunophenotyping, extracellular staining |
| Selection guide | Phospho flow cytometry antibody clone buffer selection guide | flow cytometry, immunophenotyping, intracellular staining, phosphoflow cytometry |
| CYToMORE lecture video (2016) | Assessing immunological aging using flow cytometry Presented by Victor Appay, Faculté de Médecine Pierre et Marie Curie, Pitié-Salpêtrière, France | flow cytometry, immunology, immunophenotyping |
| CYToMORE lecture video (2016) | Cytometric analysis of cytokines and transcription factors Presented by Hyun-Dong Chang, Deutsches Rheuma-Forschungszentrum, Berlin, Germany | cell signaling, flow cytometry, immunophenotyping |
| CYToMORE lecture video (2016) | Heterogeneity of tumour-infiltrating leukocytes: implications for adoptive cell transfer immunotherapy Presented by Enrico Lugli, Humanitas Clinical and Research Center, Italy | flow cytometry, immunology, immunophenotyping |
| CYToMORE lecture video (2016) | Mass cytometry in the phenomics era: an essential tool for gene/function studies Presented by Herve Luche, Centre d’Immunophénomique - CIPHE, France | immunology, immunophenotyping, mass cytometry |
| How-to video | Tutorial: Labeling a purified antibody Educational video on how to label your next antibody for imaging or fluorescence. Walk through the tutorial as Molecular Probes scientists demonstrate the protocol—including all the tips and tricks you'll want to know about for your next antibody labeling experiment. The video features Judie showing Curtis, a chemistry graduate student, how to label his monoclonal antibodies while saving time and maximizing yield. | antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| How-to video | How to site specifically label your antibody using SiteClick technology This video explains how to easily and site specifically label an antibody using an enzymatic and Click chemistry approach. This method can be applied to any intact IgG antibody and requires no antibody engineering or complex methodology. Unlike classic antibody conjugation techniques this breakthrough SiteClick technology allows for antibody conjugation with complete confidence that the label will not directly interfere with the antibody binding domain. | antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, Qdot, western detection |
| Webinar | A practical approach to antibody labeling The growing number of fluorophores available makes labeling your own antibodies a tempting proposition. But with many antibody labeling solutions available, selecting the best option can be a daunting task. In this webinar we will:
| antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF) |
| Webinar | Learn to choose the right fluorophore when designing experiments The choice of fluorophore is one of the first important decisions to make in developing an experiment. Fluorophores are compounds that emit light at a specific wavelength when they have been excited at another, lower wavelength. In this webinar we will explore:
| antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins |
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.