Tailor your SNP assay to your genotyping workflow with our custom design option
Applied Biosystems Custom TaqMan SNP Genotyping assays make it easy to utilize the power of TaqMan chemistry for detection of SNPs in any organism or genome where predesigned assays aren’t available. Integrate our gold-standard TaqMan chemistry, advanced assay design, and easy-to-use workflow into any of your genotyping applications such as screening, association, candidate region, candidate gene, or fine-mapping studies.
Our assay design pipeline is the key
The proprietary assay design process that powers our predesigned collection uses over 90+ parameters to generate optimal primer-probe designs that are wet-lab proven to maximize sensitivity and specificity. Leverage our secure assay design pipeline for your unique SNP targets by submitting confidential target sequences to the Custom TaqMan Assay Design Tool. This pipeline has generated millions of assay designs by utilizing heuristic rules deduced from both manufacturing and assay performance data.
All custom assays use TaqMan minor groove-binding (MGB) probes for superior allelic discrimination, improved signal-to-noise ratios, and design flexibility. All custom human SNP genotyping assays are functionally tested using 20 unique DNA samples to ensure assay amplification and sample clustering.
Design and order your custom assays online
Our simple online tool walks you through the process. You begin by entering a sequence, then submit the chosen target sites for assay design. Upon notification of successful design, simply add the custom assay to your card.
Custom TaqMan SNP Genotyping assays
Cat. No. – Human | Cat. No. – Non-human | Size | No. of 25 µL reactions (96 well) | No. of 10 µL reactions (96 fast) | No. of 5 µL reactions (384 well) | |
4331349 | 4332077 | Small; 40x | 300 | 750 | 1,500 | |
4332072 | 4332075 | Medium; 40x | 1,000 | 2,500 | 5,000 | |
4332073 | 4332076 | Large; 80x | 2,400 | 6,000 | 12,000 | |
Discrimination | 5' nuclease assay to discriminate between two alleles of a specific SNP for use in genotyping studies | |||||
Assay components | Each assay contains:
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Delivery times | Approximate delivery times are 4–6 days in the US and 6–10 days in Europe | |||||
Expiration date | 5 years from date of manufacture | |||||
Shipping and storage conditions | Assays ship at ambient temperature. Store at -15 to -25°C upon arrival. |
For Research Use Only. Not for use in diagnostic procedures.