Fluorescent dyes and non-fluorescent ligands are excellent affinity tags that can be recognized by labeled primary antibodies, providing an alternative to the traditional signal amplification as well as expanding the number of targets detected in multiplex experiments. Applications where anti-dye antibodies have been successfully used include DNA hybridization, immunohistochemistry, flow cytometry, ELISA, and western blotting. Their high specificity also makes them ideal reagents in applications where the signal detection is more difficult due to increased background (for example, non-specific detection of endogenous biotin in cells and tissue).
Anti-dye and anti-hapten antibodies
Catalog # | Anti-fluorophore | Host | Label |
---|---|---|---|
Anti-fluorophore antibodies and their conjugates | |||
A5760 | Alexa Fluor® 405/Cascade Blue® dye | Rabbit | None |
A11094 | Alexa Fluor® 488 dye | Rabbit | None |
A5770 | BODIPY® FL dye | Rabbit | None |
A6398 | Dansyl | Rabbit | None |
MA514696 | Fluorescein (FITC) | Mouse (clone #9) | None |
A6421 | Fluorescein/Oregon Green® dye | Mouse (clone 4-4-20) | None |
A889 | Fluorescein/Oregon Green® dye | Rabbit | None |
A6413 | Fluorescein/Oregon Green® dye | Rabbit, Fab fragment | None |
A11095 | Fluorescein/Oregon Green® dye | Goat | None |
A11090 | Fluorescein/Oregon Green® dye | Rabbit | Alexa Fluor® 488 dye |
A11096 | Fluorescein/Oregon Green® dye | Goat | Alexa Fluor® 488 dye |
A11091 | Fluorescein/Oregon Green® dye | Rabbit | Alexa Fluor® 594 dye |
A21250 | Fluorescein/Oregon Green® dye | Rabbit | R-phycoerythrin |
A982 | Fluorescein/Oregon Green® dye | Rabbit | Biotin-XX |
A21253 | Fluorescein/Oregon Green® dye | Rabbit | Horseradish peroxidase |
A5750 | Lucifer yellow dye | Rabbit | None |
A5751 | Lucifer yellow dye | Rabbit | Biotin-XX |
MA1041 | Tetramethylrhodamine (TAMRA) | Mouse (clone 5G5) | None |
A6397 | Tetramethylrhodamine (TAMRA) and Rhodamine Red™ dye | Rabbit | None |
A6399 | Texas Red® and Texas Red®-X dye | Rabbit | None |
Anti-hapten antibodies and their conjugates | |||
MA511251 | Biotin | Mouse (clone BTN.4) | None |
033700 | Biotin | Mouse (clone Z021) | None |
A6430 | Dinitrophenyl | Rabbit | None |
A6435 | Dinitrophenyl | Rabbit | Biotin-XX |
A11097 | Dinitrophenyl | Rabbit | Alexa Fluor® 488 |
A6423 | Dinitrophenyl | Rabbit | Fluorescein |
MA135779 | Dinitrophenyl | Rat (clone LO-DNP-2) | None |
A21285 | Nitrotyrosine | Rabbit | None |
MA112770 | 3-Nitrotyrosine | Mouse (clone 2A12) | None |
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For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.