 | Many Revvity plate readers including the EnVision™, ViewLux™ and VICTOR™ are compatible with Thermo Fisher Scientific assay technologies for TR-FRET, FRET, fluorescence intensity (kinetic), and fluorescence polarization. |
Before using LanthaScreen Terbium and Europium assays
Test your plate reader set-up
These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen assay.
EnVision™ multimode plate reader
Instrument configuration:
| Assay Technology | Adapta & LanthaScreen Europium | GeneBLAzer | LanthaScreen Terbium | Predictor | Z'-LYTE |
|---|---|---|---|---|---|
| Modality | TR-FRET | FRET (bottom) | TR-FRET | FP | FRET |
| Setup Guide | |||||
| Application Note | - | - | - | - | - |
| Poster | - | - | Lab Auto 08 | - | - |
| Excitation Filter (15 mm) | 340/30 (PV00215) | 400/25 | 340/30 (PV00215) | 531/25 FP | 400/25 |
| Emission 1 Filter (15 mm) | 615/8.5 | 460/25 | 495/10 (PV00315) | 579/25 FP P-pol | 460/25 |
| Emission 2 Filter (15 mm) | 665/7.5 | 535/25 | 520/25 (PV00315) | 579 FP S-pol | 535/25 |
| Dichroic Mirror | D400/630 TRF Dual or D400 TRF | D425/495 BLA Dual or D425 BLA; 50/50 General Dual or 50/50 General | D400/630 TRF Dual or D400 TRF; D400/505 LanthaScreen® Dual | D555fp/D595 Dual or D555fp | D425/495 BLA Dual or D425 BLA; 50/50 General Dual or 50/50 General |
VICTOR™ multimode plate reader
Instrument configuration:
| Assay Technology | Adapta & LanthaScreen Europium | GeneBLAzer | LanthaScreen Terbium | Predictor | Z'-LYTE |
|---|---|---|---|---|---|
| Modality | TR-FRET | FRET (bottom) | TR-FRET | FP | FRET |
| Setup Guide | Reader compatible but settings not validated | Reader not compatible | Reader not compatible | Reader not tested | |
| Application Note | - | - | - | - | - |
| Poster | - | - | - | - | - |
| Excitation Filter (15 mm) | 340/60 (built-in) | - | 340/60 (built-in) | - | NA |
| Emission 1 Filter (25 mm) | 616/8.5 | - | 495/10 (PV00325) | - | NA |
| Emission 2 Filter (25 mm) | 665/7.5 | - | 520/25 (PV00325) | - | NA |
ViewLux™ microplate imager
Instrument configuration:
| Assay Technology | Adapta & LanthaScreen Europium | GeneBLAzer | LanthaScreen Terbium | Predictor | Z'-LYTE |
|---|---|---|---|---|---|
| Modality | TR-FRET | FRET (bottom) | TR-FRET | N/A | N/A |
| Setup Guide | Reader compatible but settings not validated | Reader not tested | Reader not tested | Reader not tested | |
| Application Note | - | - | - | - | - |
| Poster | - | - | Lab Auto 08 | - | - |
| Excitation Filter (60 mm) | 340/60 | NA | 340/60 | NA | NA |
| Emission 1 Filter (60 mm) | 616/8 | NA | 495/10 (Chroma) | NA | NA |
| Emission 2 Filter (60 mm) | 671/7.8 | NA | 520/20 | NA | NA |
| Dichroic Mirror | 400 | NA | 400 | NA | NA |
* Filters (diameter) shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Revvity for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above
** Some compatible filter sets are available for purchase from Thermo Fisher Scientific. Click on selected filter sets for more information.
EnVision™ and ViewLux™ and VICTOR™ are trademarks of Revvity.
For Research Use Only. Not for use in diagnostic procedures.