The PHERAstar, PHERAstar Plus, Omega, and CLARIOstar microplate readers have received our LanthaScreen Certified Plus designation which ensures that these readers are validated to strict standards in instrument setup and assay performance.
- View the BMG LABTECH website
Before using LanthaScreen Terbium and Europium assays
Test your plate reader set-up
These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen assay.
Download Terbium assay application note
Download Europium assay application note
PHERAstar FSX Microplate Reader
- Learn more about the PHERAstar FSX Microplate Reader
Instrument configuration
| Assay technology | Adapta & LanthaScreen Europium | GeneBLAzer | LanthaScreen Terbium | Predictor | Z′-LYTE |
|---|---|---|---|---|---|
| Modality | TR-FRET | FRET (bottom) | TR-FRET | FP | FRET |
| Setup guide |  | Compatible but not tested | | Compatible but not tested | Compatible but not tested |
| Application note | — | — | — | — | — |
| Poster | — | — | — | — | — |
| Excitation filter | EX TR | 400–10 | EX TR | 532/25 | 400–10 |
| Emission 1 filter | 665–10 | 450–10 | 520–10 | 595/35 | 450–10 |
| Emission 2 filter | 620–10 | 520–10 | 490–10 | NA | 520–10 |
| Dichroic mirror | LP TR | LP 426 | LP TR | LP 565 | LP426 |
CLARIOstar/CLARIOstarPlus Microplate Readers
- Learn more about the CLARIOstar Microplate Reader
- Learn more about the CLARIOstarPlus Microplate Reader
- Documents developed for the CLARIOstar can be used for the CLARIOstarPlus
Instrument configuration
| Assay technology | Adapta & LanthaScreen Europium | GeneBLAzer | LanthaScreen Terbium | Predictor | Z'LYTE |
|---|---|---|---|---|---|
| Modality | TR-FRET | FRET (bottom) | TR-FRET | FP | FRET |
| Setup guide | | Compatible but not tested | | | |
| Application note | — | — | — | — | — |
| Poster | — | — | — | — | — |
| Excitation filter | EX TR | 400-10 | EX TR | 540-20 | 400-10 |
| Emission 1 filter | 665-10 | 450-10 | 520-10 | 590-20 | 450-10 |
| Emission 2 filter | 620-10 | 520-10 | 490-10 | NA | 520-10 |
| Dichroic mirror | LP TR | LP 426 | LP TR | LP 565 | LP 426 |
*LVF monochromator setting
+Linear variable dichroic setting
PHERAstar FS Microplate Reader
Instrument configuration
| Assay technology | Adapta & LanthaScreen Europium | GeneBLAzer | LanthaScreen Terbium | Predictor | Z′-LYTE |
|---|---|---|---|---|---|
| Modality | TR-FRET | FRET (bottom) | TR-FRET | FP | FRET |
| Setup guide | | | | | |
| Application note | — | View | — | — | — |
| Poster | — | — | — | — | — |
| Excitation filter | 337 | 405 | 337 | 532/25 | 405 |
| Emission 1 filter | 665 | 460 | 520 | 595/35 | 460 |
| Emission 2 filter | 620 | 530 | 490 | 595/35 | 530 |
| Dichroic mirror | Included | Included | Included | Included | Included |
| Optic module | HTRF® | FI 405 530 460 | LanthaScreen | FP rhodamine | FI 405 530 460 |
PHERAstar/PHERAstar Plus Microplate Readers
Instrument configuration
| Assay technology | Adapta & LanthaScreen Europium | GeneBLAzer | LanthaScreen Terbium | Predictor | Z′-LYTE |
|---|---|---|---|---|---|
| Modality | TR-FRET | FRET (bottom) | TR-FRET | FP | FRET |
| Setup guide | | PHERAstar Plus compatible not tested | | | |
| Application note | — | — | — | — | — |
| Poster | — | — | — | — | — |
| Excitation filter | 337 | — | 337 | 540 | 400 |
| Emission 1 filter | 665 | — | 520 | 590 | 440 |
| Emission 2 filter | 620 | — | 490 | 590 | 520 |
| Dichroic mirror | Included | — | Included | Included | Included |
| Optic module | HTRF® | — | LanthaScreen | Red FP | NA |
FLUOstar Omega & POLARstar Omega Microplate Readers
- Learn more about the FLUOstar Omega and POLARstar Omega
Instrument configuration
| Assay technology | Adapta & LanthaScreen Europium | GeneBLAzer | LanthaScreen Terbium | Predictor | Z′-LYTE |
|---|---|---|---|---|---|
| Modality | TR-FRET | FRET (bottom) | TR-FRET | FP | FRET |
| Setup guide | | | | ! POLARstar not compatible | |
| Application note | — | — | — | — | — |
| Poster | — | — | — | — | — |
| Excitation filter | 337 | 400 | 337 | NA | 400 |
| Emission 1 filter | 665 | 440 | 520 | NA | 440 |
| Emission 2 filter | 620 | 520 | 490 | NA | 520 |
Note: for the Adapta assay and LanthaScreen Europium and Terbium assays both the FLUOstar Omega and POLARstar Omega need BMG LABTECH's TRF head accessory
! The FLUOstar Omega is not equipped to perform the Predictor assay and the POLARstar Omega is not compatible
FLUOstar OPTIMA & POLARstar OPTIMA Microplate Readers
Instrument configuration
| Assay technology | Adapta & LanthaScreen Europium | GeneBLAzer | LanthaScreen Terbium | Predictor | Z′-LYTE |
|---|---|---|---|---|---|
| Modality | TR-FRET | FRET (bottom) | TR-FRET | FP | FRET |
| Setup guide | Readers not compatible | | Readers not compatible | ! POLARstar not compatible | |
| Application note | — | — | — | — | — |
| Poster | — | — | — | — | — |
| Excitation filter | — | 400 | — | NA | 400 |
| Emission 1 filter | — | 440 | — | NA | 440 |
| Emission 2 filter | — | 520 | — | NA | 520 |
! The FLUOstar OPTIMA is not equipped to perform the Predictor assay and the POLARstar OPTIMA is not compatible
* Filters shown as center wavelength (nm); Contact BMG LABTECH for information on ordering optics modules & filters suitable with the microplate readers listed above.
HTRF® is a registered trademark of Cisbio Assays
For Research Use Only. Not for use in diagnostic procedures.