Isolated from the thermophilic bacterium Thermus aquaticus, Taq DNA polymerase is one of the best-known thermostable DNA polymerases used in PCR amplification of DNA targets. The native enzyme is purified from Thermus aquaticus YT1. The recombinant enzyme is a cloned version that is purified from E. coli. Therefore, the native Taq DNA polymerase is often preferred for amplification of bacterial DNA sequences homologous to E. coli sequences.
Find the DNA polymerase for your PCR application
Since the first report on PCR using Taq DNA polymerase, better-performing DNA polymerases are continually being developed to meet the needs of increasingly challenging research applications. The enzymes listed below help improve PCR performance in a variety of aspects such as sensitivity, specificity, accuracy, and inhibitor tolerance.
Sensitivity (yield) | Specificity (hot start) | Accuracy (vs. Taq fidelity) | Inhibitor tolerance | GC-rich template | Amplification range | Low residual bacterial gDNA | |
---|---|---|---|---|---|---|---|
(e.g., for low-abundance targets) | (e.g., for genotyping) | (e.g., for cloning, mutagenesis) | (e.g., for clinical research samples) | (e.g., for promoter sequences) | (e.g., for long DNA targets) | (e.g., for microbiome studies) | |
Platinum II Taq DNA Polymerase | +++ | +++ | 1x | +++ | +++ | 5 kb | ≤1 copy/50 μL rxn |
Platinum SuperFi II DNA Polymerase | +++ | +++ | >300x | +++ | +++ | 20 kb | ≤1 copy/50 μL rxn |
Platinum Taq DNA Polymerase, DNA-free | ++ | +++ | 1x | + | + | 5 kb | ≤0.01 copy/50 μL rxn |
Standard Taq DNA polymerase | + | + | 1x | + | + | 5 kb | Not tested |
DreamTaq DNA Polymerase
If you just need a standard PCR enzyme with enhanced performance, check out Thermo Scientific DreamTaq DNA Polymerases, which offer:
- Longer amplicons and higher yields than conventional Taq polymerases
- Stand-alone and master-mix formats
- Direct gel loading options
Order Taq DNA polymerases for PCR
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For Research Use Only. Not for use in diagnostic procedures.