Oligonucleotides are the starting point for many of today’s biology research, drug discovery, and diagnostics applications. These highly technical applications demand high-quality oligonucleotides for success. Our oligos are made to your specifications with rigorous quality control and quick turnaround for use in a variety of applications, including PCR, cloning, sequencing, and gene detection.
Explore the easy-to use ordering portals for your tube and plate oligos, our complex and large-scale oligo services, and additional resources below.
Available custom oligo formats
| Standard oligos in tubes | Standard oligos in plates | Complex and large-scale oligos | |
|---|---|---|---|
| Scales | 25 nmol, 50 nmol, 200 nmol, 1 µmol, 10 µmol | Milligram to >15 gram final yield | |
| Lengths | Up to 100 bases | ||
| Purification | Desalted, cartridge, HPLC, PAGE | Desalted, HPLC, RP-HPLC, IEX-HPLC, Dual-HPLC | |
| Modifications | 5’, internal, 3’ | ||
| Formats |
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| Custom formulations and packaging solutions |
| Ordering | Enter individually or bulk upload up to 200 sequences at once | Upload each plate separately using our template |
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| Shipping | Standard or next-day delivery, with free overnight shipping* | Standard delivery, with free overnight shipping* | |
| Additional resources | Modification and configuration options Online ordering guide | Complex and large-scale capabilities | |
All oligos are manufactured using controlled procedures certified against ISO 9001:2015 and ISO 13485:2016 standards.
Custom DNA oligos brochure
For a comprehensive guide on the small- and large-scale custom solutions and end-to-end services designed with your unique application in mind, access the Custom DNA oligos brochure.
Our custom oligonucleotide synthesis process
Our custom DNA oligos are synthesized on a highly automated, computer-controlled system using standard cyano-ethyl phosphoramidite chemistry. Post-synthesis QC employs electrospray ionization mass spectrometry (ESI MS) for all oligos. As a standard practice, 100% of synthesized oligos are measured for quality control whether provided in tubes or in plates.
Choose DNA oligos for your application
While our value oligo format represents our most economical and fastest way to order (25 or 50 nmol in tube formats with a range of purification options to suit your applications), if your research demands a scale larger than 50 nmol, a different format, or requires 5′, 3′, or internal modifications, then our standard DNA oligo formats have you covered (Table 1).
Table 1. Which kind of oligos are right for me?
Choosing the synthesis scale and purification option that is well suited for your application depends on the nature of your downstream applications. We can help you choose the right oligos and purification methods for your application (Table 2).
Table 2. Recommended oligo processing options by application
| Application | Desalted | Cartridge | HPLC | PAGE |
|---|---|---|---|---|
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| Standard PCR | ||||
| Specialty PCR | ||||
| Cloning | ||||
| cDNA library construction | ||||
| Sequencing | ||||
| Fluorescent sequencing | ||||
| Next generation sequencing | ||||
| Mutagenesis | ||||
| Antisense | ||||
| Gel shift assays | ||||
| Microarrays |
Looking for pre-defined oligo sequences?
In addition to our custom synthesis options for oligos, primers, probes, and genes, we offer random primers, oligo (dT) primers, 5’ labeled primers, gene specific primers for sequencing, forward and reverse sequencing primers and kits.
Find everyday lab essentials to power your PCR every step of the way. Shop now
* This promotion is open to customers in the US (excluding Puerto Rico) and Canada. Discount will apply to qualifying orders received by Life Technologies no later than December 31, 2024. Discount applies to list price in effect at the time order is received by Life Technologies. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.
For Research Use Only. Not for use in diagnostic procedures.