Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
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| 4 | 568 | Texas Red | 579 | 603 | (in buffer) 3 (in antifade) 4 | microscopy; flow cytometry; fluorescence microplate reader |
Invitrogen Alexa Fluor 568 dye is a bright, red-orange-fluorescent dye with excitation designed for use with the 568 nm laser line on the Ar-Kr mixed-gas laser and matches well with RFP filter sets. For stable signal generation in imaging and flow cytometry, Alexa Fluor 568 dye is pH-insensitive over a wide molar range. In addition, it is used in stochastic optical reconstruction microscopy (STORM)—as a reporter in dSTORM.
Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 568 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
We offer Alexa Fluor 568 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.
Search Alexa Fluor 568 secondary antibodies Protein Labeling Reagents Selection Guide (NHS ester, maleimide, etc.)
Additional products
- Antibody and protein labeling kits
- Super-resolution microscopy (SRM) applications
- Streptavidin conjugates for signal amplification
- Phalloidin conjugates for actin staining
- Cell and Neuronal Tracing
- Annexin V conjugates for apoptosis imaging
- Tyramide conjugates for ultimate sensitivity
- Labeled nucleotides for hybridization probes
Click image to enlarge
Flow cytometry analysis of human mononuclear cells using Zenon Alexa Fluor 568 Labeling Kit. An antibody complex was prepared using the Zenon Alexa Fluor 568 Mouse IgG1 Labeling Kit and mouse anti-human CD4. Human mononuclear cells were stained with the antibody complex and analyzed by flow cytometry using a 532 nm Laser.
Co-stain of neuron with Dextran and Hydrazide. Confocal image stack of a 10,000 MW Calcium Green dextran-labeled climbing fiber in a sagittal cerebellar slice, showing incoming axon and terminal arborization (in yellow). The Purkinje cell innervated by this fiber was labeled with Alexa Fluor 568 Hydrazide.
Three-color staining of muntjac cells with Alexa Fluor Phalloidin conjugate and Zenon Mouse Labeling Kit. Fixed and permeabilized muntjac skin fibroblasts were stained with Alexa Fluor 488 Phalloidin, alpha-Tubulin Monoclonal Antibody, and anti-Cdc6 Polyclonal Antibody. The alpha-Tubulin antibody was prelabeled with the Zenon Alexa Fluor 568 Mouse IgG1 Labeling Kit, and anti-Cdc6 antibody was prelabeled with Zenon Alexa Fluor 350 Mouse IgG1 Labeling Kit.
Additional resources
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
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