Table 9.2 SYPRO and Coomassie Fluor luminescent and fluorescent protein gel stains.
| Dye (Cat #) | Ex/Em * | Major Applications | Features |
|---|
| SYPRO Ruby protein gel stain (S12000, S12001, S21900) | 280, 450/610 | 2D gels, IEF gels, 1D SDS-PAGE | - High sensitivity (1–2 ng/band; comparable to silver staining)
- Linear quantitation range over three orders of magnitude
|
| SYPRO Orange protein gel stain (S6650, S6651) | 300, 470/570 | 1D SDS-PAGE | - Very good sensitivity (4–8 ng/band; higher than Coomassie brilliant blue staining)
- Little protein-to-protein variability
- Linear quantitation range over three orders of magnitude
|
| SYPRO Red protein gel stain (S6653, S6654) | 300, 550/630 | 1D SDS-PAGE | - Very good sensitivity (4–8 ng/band; higher than Coomassie brilliant blue staining)
- Little protein-to-protein variability
- Linear quantitation range over three orders of magnitude
|
| SYPRO Tangerine protein gel stain (S12010) | 300, 490/640 | 1D SDS-PAGE, blotting applications, zymography, electroelution | - Very good sensitivity (4–8 ng/band; higher than Coomassie brilliant blue staining)
- Little protein-to-protein variability
- Linear quantitation range over three orders of magnitude
- No fixation required
|
| Coomassie Fluor Orange protein gel stain (C33250, C33251) | 300, 470/570 | 1D SDS-PAGE | - Premixed and ready-to-use solution
- Very good sensitivity (8 ng/band; higher than Coomassie brilliant blue staining)
- Little protein-to-protein variability
- Linear quantitation range over at least two orders of magnitude
|
| All SYPRO gel and blot stains are compatible with Edman sequencing and mass spectrometry. * Excitation (Ex) and emission (Em) maxima, in nm. For maximum sensitivity, use excitation sources and optical filters matched to these values. |