Biotin quantitation kits are used for determining biotinylation levels and efficiency by either colorimetric or fluorescence detection methods. These kits contain the simple and accurate HABA dye used to measure the amount of biotin attached to proteins or other biomolecules. Individual fluorescent quantitation reagents are also available for detecting and quantitating biotin-binding sites. Now you can determine the molar ratio of biotin to a labeled antibody molecule in an easy, efficient manner.
Following biotin labeling, the HABA calculator can be used to determine the average number of biotin groups per protein molecule.
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- Biotin quantitation selection guide
Biotin quantitation selection guide
| Product | Features | Detection method | SKU |
|---|---|---|---|
| Fluorescence Biotin Quantitation Kit | Fluorescent assay reagent and HABA dye for rapid and sensitive determination of biotinylation levels in labeled antibodies and other proteins with minimal sample quantities | Fluorescent | 46610 |
| Pierce Biotin Quantitation Kit | A simple, colorimetric method for determining biotinylation efficiency | Colorimetric | 28005 |
| HABA | HABA dye reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in antibodies and other proteins | Colorimetric | 28010 |
| FluoReporter Biotin Quantitation Assay Kit | Fluorometric assay for accurately determining the number of biotin labels on a protein with higher sensitivity than the HABA biotin binding assay | Fluorescent | F30751 |
Click image to enlarge
Fluorescence Biotin Quantitation Kit.Fluorescence (green stars) of the avidin dye increases when HABA dye (H) is displaced by biotin (B) contributed by a known amount of biotinylated protein. Measurement is made in black opaque 96-well microplates with a fluorescence plate reader (excitation 494 nm; emission 520 nm).
Click image to enlarge
Biotin Quantitation Kit. When HABA is bound to avidin, the complex absorbs light at 500 nm (e = 34,000/M cm). When a biotin-containing sample is added, the biotin binds strongly to avidin and displaces the weakly bound HABA resulting in a decrease in absorbance relative to the amount of HABA displaced.
Biotin-4-fluorescein. Quantitation of biotin-binding sites with 8 nM biotin-4-fluorescein. Both the fluorescence and absorbance of biotin-4-fluorescein are quenched upon binding to one of the four biotin-binding sites of streptavidin (pink), avidin (dark blue), or the Alexa Fluor 633 streptavidin (orange) and streptavidin, alkaline phosphatase (light blue). As a result, when a known concentration of biotin-4-fluorescein is added to a known amount of streptavidin, one can estimate the number of biotin-binding sites.
| Product | Features | Detection method | SKU |
|---|---|---|---|
| Fluorescence Biotin Quantitation Kit | Fluorescent assay reagent and HABA dye for rapid and sensitive determination of biotinylation levels in labeled antibodies and other proteins with minimal sample quantities | Fluorescent | 46610 |
| Pierce Biotin Quantitation Kit | A simple, colorimetric method for determining biotinylation efficiency | Colorimetric | 28005 |
| HABA | HABA dye reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in antibodies and other proteins | Colorimetric | 28010 |
| FluoReporter Biotin Quantitation Assay Kit | Fluorometric assay for accurately determining the number of biotin labels on a protein with higher sensitivity than the HABA biotin binding assay | Fluorescent | F30751 |
Click image to enlarge
Fluorescence Biotin Quantitation Kit.Fluorescence (green stars) of the avidin dye increases when HABA dye (H) is displaced by biotin (B) contributed by a known amount of biotinylated protein. Measurement is made in black opaque 96-well microplates with a fluorescence plate reader (excitation 494 nm; emission 520 nm).
Click image to enlarge
Biotin Quantitation Kit. When HABA is bound to avidin, the complex absorbs light at 500 nm (e = 34,000/M cm). When a biotin-containing sample is added, the biotin binds strongly to avidin and displaces the weakly bound HABA resulting in a decrease in absorbance relative to the amount of HABA displaced.
Biotin-4-fluorescein. Quantitation of biotin-binding sites with 8 nM biotin-4-fluorescein. Both the fluorescence and absorbance of biotin-4-fluorescein are quenched upon binding to one of the four biotin-binding sites of streptavidin (pink), avidin (dark blue), or the Alexa Fluor 633 streptavidin (orange) and streptavidin, alkaline phosphatase (light blue). As a result, when a known concentration of biotin-4-fluorescein is added to a known amount of streptavidin, one can estimate the number of biotin-binding sites.
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Avidin-Biotin technical handbook
Everything you need to biotinylate, purify, or detect proteins
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