Antibody purification involves isolation of antibody from serum (polyclonal antibody), ascites fluid, or from the culture supernatant of a hybridoma cell line (monoclonal antibody). Protein L is a recombinant protein expressed in Escherichia coli (35.8 kDa; 4 IgG binding sites) and is recommended for human or mouse monoclonal antibodies known to have appropriate kappa light chains.
We offer two base supports and a variety of formats to purify antibodies using the Protein L ligand. Our portfolio is designed to meet small-scale (screening) to pilot-scale needs.
- Multiple formats—magnetic beads, loose resin, spin columns and kits, and FPLC cartridges enable antibody purification from microgram to kilogram scales
- High performance—resins are designed to maximize protein yield and reduce background
- Economical—pricing is similar to or better than our leading competitors
Choose the right Protein L product for antibody purification
Product links in the last row of each column
| Pierce Protein L Magnetic Beads | Pierce Protein L Agarose | Pierce Protein L Plus Agarose | |
| Bead or resin size | 1 μm | 45–165 μm | 45–165 μm |
| Binding capacity | ≥110 μg human IgG/mg beads | 5–10 mg human IgG/mL resin | 10–20 mg human IgG/mL resin |
| Maximum linear flow rate | N/A | 700 cm/hr | 700 cm/hr |
| Support | Magnetite coated polymer | Crosslinked 6% beaded agarose | Crosslinked 6% beaded agarose |
| Recommended application scale | screening | batch, pilot | batch, pilot |
| Formats available | loose beads | loose beads, pre-packed spin columns and kits, chromatography cartridges | loose beads |
| Order product(s) | 88849 | 20510 (loose resin) 89928 (1 mL cartridge) 89929 (5 mL cartridge) | 20520 (loose resin) |
Resources
For Research Use Only. Not for use in diagnostic procedures.