Antibody purification involves isolation of antibody from serum (polyclonal antibody), ascites fluid, or from the culture supernatant of a hybridoma cell line (monoclonal antibody). Protein A ligand is recommended specifically for polyclonal IgG from rabbit, pig, dog, or cat serum. It binds the heavy chain constant region (Fc) of IgG (CH2-CH3 region). Native Protein A is purified from Staphylococcus aureus (46.7 kDa; 4 IgG-binding sites). The POROS MabCapture A resins feature a recombinant Protein A (rPA) ligand produced in yeast that can be scaled up for bioprocess applications.
The MabCaptureC™ High Capacity Protein A is a high-performance chromatography resin for capturing mAbs and recombinant Fc-tagged fusion proteins. It provides high specific binding capacity and enhanced alkaline stability characteristics under clean-in-place conditions (CIP), for a more robust purification and resin regeneration.
We offer a wide selection of base supports and formats to purify antibodies using the Protein A ligand. Our portfolio is designed to meet small-scale (screening) up to large-scale (bioprocess) needs.
- Multiple formats—magnetic beads, loose resin, spin columns and kits, FPLC cartridges, and 96-well filter plates enable antibody purification from microgram to kilogram scales
- Alkaline stability—engineered to withstand common CIP procedures with 0.2 M NaOH enabling efficient cleaning of the chromatography resin
- High performance—resins are designed to maximize protein yield and reduce background
- Economical—pricing is similar to or better than our leading competitors
Choose the right Protein A product for antibody purification
Product links in the last row of each column
| Dynabeads Protein A Magnetic Beads | Pierce Protein A Agarose | Pierce Protein A Plus Agarose | Pierce Recombinant Protein A Agarose | MabCaptureC™ High Capacity Protein A, Alkaline-Stable | |
| Bead or resin size | 2.8 μm | 45–165 μm | 45–165 μm | 45–165 μm | 75 μm |
| Binding capacity | 250–300 μg human IgG/mL beads | 12–19 mg human IgG/mL resin | 34 mg human IgG/mL resin | 15–17 mg human IgG/mL resin | 58 mg/ml at 5 min residence (80 mg/ml at 10 min residence) – 10% breakthrough |
| Maximum linear flow rate | N/A | 700 cm/hr | 700 cm/hr | 700 cm/hr | 300 cm/hr or less (150 cm/hr recommended) |
| Support | Monosized, super paramagnetic polystyrene beads | Crosslinked 6% beaded agarose | Crosslinked 6% beaded agarose | Crosslinked 6% beaded agarose | Highly cross-linked agarose |
| Recommended application scale | screening | batch, pilot | screening, batch | batch | batch, pilot, process |
| Formats available | loose beads | loose beads, chromatography cartridges | loose beads, pre-packed spin columns and kits, 96-well filter plates | loose beads | loose beads |
| Order product(s) | 10001D | 20333 (loose resin) 88924 (1 mL cartridge) 89925 (5 mL cartridge) 44667 (spin kit) | 22810 (loose resin) 45202 (spin plates) | 20365 | A53031, 10 mL 50% slurry A53033, 50 mL 50% slurry A53034, 200 mL 50% slurry 1963662250, 250 mL 50% slurry 196366201L, 1 L 50% slurry 196366205L, 5 L 50% slurry 196366210L, 10 L 50% slurry |
For Research Use Only. Not for use in diagnostic procedures.