Table 17.2 Spectroscopic properties of MANT-ATP in aqueous solution (pH 8).
| Parameter | Value | Notes |
|---|---|---|
| Absorption maximum | 356 nm | Stronger absorption at shorter wavelengths (λmax = 255 nm) |
| Molar extinction coefficient (εmax) | 5800 cm-1M-1 | 23,000 cm-1M-1 at 255 nm |
| Fluorescence emission maximum | 448 nm | Shifts 10–20 nm shorter in nonpolar solvents and upon binding to most proteins |
| Fluorescence quantum yield | 0.22 | Increases in nonpolar solvents and upon binding to most proteins |
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