Discover the future of mAb manufacturing
The Efficient-Pro Medium is an advanced, next-generation monoclonal antibody medium designed to work optimally with CHO cell lines. The medium pairs with two innovative, single-part feeds, formulated specifically for use in key bioproduction cell lines.
Developed with chemically defined and animal origin-free formulations, the Efficient-Pro Medium and feeds have been designed to help you maintain batch-to-batch consistency. Additionally, through Thermo Fisher’s global network of harmonized facilities and manufacturing redundancy, you can reduce the risk of supply disruption, enabling you to focus on your process.
The Efficient-Pro system can improve titers in a monoclonal antibody manufacturing workflow
Increase titers and enhance your mAb manufacturing workflow
Optimize your product quality with a monoclonal antibody medium and feed system that has been shown to help elevate titers in mAb production processes.
The basal medium is a high-performing mAb cell culture solution that can help boost the performance of your CHO cell line. When paired with our two complementary feeds, Efficient-Pro Feed 1 and Efficient-Pro Feed 2, the complete medium and feed system can effectively improve titers in CHO-K1 and CHO-S/DG44 cells, respectively.
Together, the medium and cell-specific feed can help increase titers. As shown in figures 1 and 2, titer was improved with Efficient-Pro Medium and Feed, and that performance was optimized by feed titration, showing that 1% Feed 1 (or 1.5% Feed 2) provided optimal results.
Figure 1: Efficient-Pro Feed 1 showed comparable productivity to the competitor. Efficient-Pro Medium and Feed 1 maintained high viability and lower cell density indicating a higher specific productivity compared to the competitor.
Figure 2: Efficient-Pro Feed 2 resulted in 44% higher protein titer over the competitor. Efficient-Pro Medium and Feed 2 maintained viability above 90% during the 14-day run with comparable cell density to the competitor.
Figure 3: CHO-K1 GS titer with Efficient-Pro Medium and Efficient-Pro Feed 2 compared to other media and feeds. CHO-K1 GS cells were evaluated in an Amber 15 cell culture bioreactor system. Bioreactor vessels were set up in triplicate. Efficient-Pro Medium and Efficient-Pro Feed 2 performed better than Gibco ExpiCHO Stable Production Medium, Gibco Dynamis, and competitor medium and feed. Note, all media were supplemented with 2 mg/L insulin.
The Efficient-Pro system can help you achieve desired mAb quality profiles
Improve protein quality and hit your CQA targets
The Efficient-Pro system is designed to help you achieve your mAb production critical quality attribute targets, including optimized glycosylation patterns, charge-variant profiles, and protein aggregation levels.
Figure 4: Charge variant profile of CHO-K1 cells with Efficient-Pro Feed 1. Using Efficient-Pro Medium and Feed 1 resulted in a preferred charge variant profile with a more neutral profile than competitor medium and feed.
Figure 5: Charge profile variant of CHO-S cells with Efficient-Pro Feed 2. Using Efficient-Pro Medium and Feed 2 resulted in a preferred charge variant profile with a more neutral profile than competitor medium and feed.
The Efficient-Pro system supports viable cell density and maximizes monoclonal antibody process productivity
Targeted productivity enhancements can streamline scale-up
Biopharmaceutical manufacturers can sustain higher levels of specific productivity for a range of CHO cell types for more efficient antibody production.
The ready-to-use medium and feeds are nutritionally optimized to facilitate high cell viability, low cell density, and lower levels of toxic metabolite production, helping drive the critical productivity enhancements required when transitioning to commercial manufacture.
Figure 6: Protein production in CHO-K1 cells using Efficient-Pro Feed 1. Efficient-Pro Feed 1 enhanced protein production by 147% compared to the competitor with CHO-K1 cells. Efficient-Pro Medium and Feed 1 maintained high-visibility and lower cell density indicating a higher specific productivity compared to the competitor.
Streamline your mAb production with the Gibco Advanced Granulation Technology (AGT) media format
All components of the Efficient-Pro system are available in liquid format and our proprietary AGT format, which can help simplify reconstitution and streamline your mAb manufacturing workflow.
The AGT medium and feeds are pH and osmolality preadjusted and designed for rapid dissolution, facilitating accelerated media preparation, reducing time and labor costs, and reduced risk of manual errors.
For faster scale-up, try the Efficient-Pro system in our AGT dry media format. The AGT format offers simple reconstitution and animal origin free (AOF), chemically defined properties that help streamline regulatory processes.
The Efficient-Pro Medium and Feeds are protein-free. It is recommended to maintain supplementation with growth factors, an anti-clumping agent (Pluronic), peptones, GS supplement, GlutaMAX, or L-Glutamine, if currently used in the control process.
Since the Efficient-Pro Feeds are nutrient-rich, a lower feed concentration may be sufficient, and a feed titration can help with process optimization.
The Efficient-Pro system can improve titers in a monoclonal antibody manufacturing workflow
Increase titers and enhance your mAb manufacturing workflow
Optimize your product quality with a monoclonal antibody medium and feed system that has been shown to help elevate titers in mAb production processes.
The basal medium is a high-performing mAb cell culture solution that can help boost the performance of your CHO cell line. When paired with our two complementary feeds, Efficient-Pro Feed 1 and Efficient-Pro Feed 2, the complete medium and feed system can effectively improve titers in CHO-K1 and CHO-S/DG44 cells, respectively.
Together, the medium and cell-specific feed can help increase titers. As shown in figures 1 and 2, titer was improved with Efficient-Pro Medium and Feed, and that performance was optimized by feed titration, showing that 1% Feed 1 (or 1.5% Feed 2) provided optimal results.
Figure 1: Efficient-Pro Feed 1 showed comparable productivity to the competitor. Efficient-Pro Medium and Feed 1 maintained high viability and lower cell density indicating a higher specific productivity compared to the competitor.
Figure 2: Efficient-Pro Feed 2 resulted in 44% higher protein titer over the competitor. Efficient-Pro Medium and Feed 2 maintained viability above 90% during the 14-day run with comparable cell density to the competitor.
Figure 3: CHO-K1 GS titer with Efficient-Pro Medium and Efficient-Pro Feed 2 compared to other media and feeds. CHO-K1 GS cells were evaluated in an Amber 15 cell culture bioreactor system. Bioreactor vessels were set up in triplicate. Efficient-Pro Medium and Efficient-Pro Feed 2 performed better than Gibco ExpiCHO Stable Production Medium, Gibco Dynamis, and competitor medium and feed. Note, all media were supplemented with 2 mg/L insulin.
The Efficient-Pro system can help you achieve desired mAb quality profiles
Improve protein quality and hit your CQA targets
The Efficient-Pro system is designed to help you achieve your mAb production critical quality attribute targets, including optimized glycosylation patterns, charge-variant profiles, and protein aggregation levels.
Figure 4: Charge variant profile of CHO-K1 cells with Efficient-Pro Feed 1. Using Efficient-Pro Medium and Feed 1 resulted in a preferred charge variant profile with a more neutral profile than competitor medium and feed.
Figure 5: Charge profile variant of CHO-S cells with Efficient-Pro Feed 2. Using Efficient-Pro Medium and Feed 2 resulted in a preferred charge variant profile with a more neutral profile than competitor medium and feed.
The Efficient-Pro system supports viable cell density and maximizes monoclonal antibody process productivity
Targeted productivity enhancements can streamline scale-up
Biopharmaceutical manufacturers can sustain higher levels of specific productivity for a range of CHO cell types for more efficient antibody production.
The ready-to-use medium and feeds are nutritionally optimized to facilitate high cell viability, low cell density, and lower levels of toxic metabolite production, helping drive the critical productivity enhancements required when transitioning to commercial manufacture.
Figure 6: Protein production in CHO-K1 cells using Efficient-Pro Feed 1. Efficient-Pro Feed 1 enhanced protein production by 147% compared to the competitor with CHO-K1 cells. Efficient-Pro Medium and Feed 1 maintained high-visibility and lower cell density indicating a higher specific productivity compared to the competitor.
Streamline your mAb production with the Gibco Advanced Granulation Technology (AGT) media format
All components of the Efficient-Pro system are available in liquid format and our proprietary AGT format, which can help simplify reconstitution and streamline your mAb manufacturing workflow.
The AGT medium and feeds are pH and osmolality preadjusted and designed for rapid dissolution, facilitating accelerated media preparation, reducing time and labor costs, and reduced risk of manual errors.
For faster scale-up, try the Efficient-Pro system in our AGT dry media format. The AGT format offers simple reconstitution and animal origin free (AOF), chemically defined properties that help streamline regulatory processes.
The Efficient-Pro Medium and Feeds are protein-free. It is recommended to maintain supplementation with growth factors, an anti-clumping agent (Pluronic), peptones, GS supplement, GlutaMAX, or L-Glutamine, if currently used in the control process.
Since the Efficient-Pro Feeds are nutrient-rich, a lower feed concentration may be sufficient, and a feed titration can help with process optimization.
Ordering information
Media evaluation, analytics and custom development services
From antibodies to advanced therapies, collaborate with a knowledgeable and trusted pioneer of bioprocessing innovation, providing customized solutions that help empower you to meet your project goals. Take advantage of Gibco Media by Design Services to customize a catalog medium to meet your exact specifications. We also offer convenient media and feed panel evaluations; comprehensive bioproduction analytics services; and flexible full custom media development services.
Related resources
Application note: Efficient-Pro Medium and Feeds improve productivity in CHO-K1, CHO-S, and DG44 cells
Application note: Efficient-Pro Medium and Feeds deliver strong product quality while enhancing productivity in CHO-K1 and CHO-S cells
Guidebook: Efficient-Pro: Discover the future of mAb manufacturing
Guide: Efficient-Pro Medium and Feeds Evaluation
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