Invitrogen

CD8a Monoclonal Antibody (53-6.7), PE-eFluor™ 610, eBioscience™

FIGURE: 1 / 99

CD8a Antibody (61-0081-82) in Flow

Staining of mouse splenocytes with Anti-Mouse CD3 PerCP-eFluor® 710 (Product # 46-0032-82) and 0.25 µg of Rat IgG2a K Isotype Control PE-eFluor® 610 (Product # 61-4321-82) (left) or 0.25 µg of Anti-Mouse CD8a PE-eFluor® 610 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

61-0081-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 91 publications 91 publications

Functional Assay (Functional)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Hamster, Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-eFluor™ 610, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

53-6.7

Conjugate

PE-eFluor™ 610 PE-eFluor™ 610 PE-eFluor™ 610

Excitation/Emission Max

565/606 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574524

Product Specific Information

Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.

Applications Reported: This 53-6.7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 53-6.7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Product References

Protocol for PPP1R15A-inhibited mouse model establishment with subcutaneous B16F1 tumor and single-cell analysis.

STAR protocols

Wang R,Wang M,Pei S,Zhang Y,Guo S,Guo W,Wu Z,Wang H,Li Y,Zhu Y,Meng LH,Lang J,Jin G,Xiao Y,Hu L,Kong X

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Fri Dec 15 00:00:00 EST 2023

Deletion of CD36 exhibits limited impact on normal hematopoiesis and the leukemia microenvironment.

Cellular & molecular biology letters

Meng Y,Pospiech M,Ali A,Chandwani R,Vergel M,Onyemaechi S,Yaghmour G,Lu R,Alachkar H

61-0081-82 was used in Flow cytometry/Cell sorting to investigate whether targeting CD36 has any detrimental impact on normal hematopoietic cells.

Wed May 24 00:00:00 EDT 2023

Chronic T cell proliferation in brains after stroke could interfere with the efficacy of immunotherapies.

The Journal of experimental medicine

Heindl S,Ricci A,Carofiglio O,Zhou Q,Arzberger T,Lenart N,Franzmeier N,Hortobagyi T,Nelson PT,Stowe AM,Denes A,Edbauer D,Liesz A

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

A Biological Circuit Involving Mef2c, Mef2d, and Hdac9 Controls the Immunosuppressive Functions of CD4+Foxp3+ T-Regulatory Cells.

Frontiers in immunology

Di Giorgio E,Wang L,Xiong Y,Christensen LM,Akimova T,Han R,Samanta A,Trevisanut M,Brancolini C,Beier UH,Hancock WW

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Fri Dec 17 00:00:00 EST 2021

Local Sustained GM-CSF Delivery by Genetically Engineered Encapsulated Cells Enhanced Both Cellular and Humoral SARS-CoV-2 Spike-Specific Immune Response in an Experimental Murine Spike DNA Vaccination Model.

Vaccines

Vernet R,Charrier E,Cosset E,Fièvre S,Tomasello U,Grogg J,Mach N

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Mon May 10 00:00:00 EDT 2021

Cancer immunotherapy by NC410, a LAIR-2 Fc protein blocking human LAIR-collagen interaction.

eLife

Ramos MIP,Tian L,de Ruiter EJ,Song C,Paucarmayta A,Singh A,Elshof E,Vijver SV,Shaik J,Bosiacki J,Cusumano Z,Jensen C,Willumsen N,Karsdal MA,Liu L,Langermann S,Willems S,Flies D,Meyaard L

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Mon Jun 14 00:00:00 EDT 2021

Sensing of endogenous nucleic acids by ZBP1 induces keratinocyte necroptosis and skin inflammation.

The Journal of experimental medicine

Devos M,Tanghe G,Gilbert B,Dierick E,Verheirstraeten M,Nemegeer J,de Reuver R,Lefebvre S,De Munck J,Rehwinkel J,Vandenabeele P,Declercq W,Maelfait J

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Mon Jul 06 00:00:00 EDT 2020

Glufosinate constrains synchronous and metachronous metastasis by promoting anti-tumor macrophages.

EMBO molecular medicine

Menga A,Serra M,Todisco S,Riera-Domingo C,Ammarah U,Ehling M,Palmieri EM,Di Noia MA,Gissi R,Favia M,Pierri CL,Porporato PE,Castegna A,Mazzone M

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Wed Oct 07 00:00:00 EDT 2020

HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment.

Journal of Cancer

Kim YD,Park SM,Ha HC,Lee AR,Won H,Cha H,Cho S,Cho JM

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

TIAM2S Mediates Serotonin Homeostasis and Provokes a Pro-Inflammatory Immune Microenvironment Permissive for Colorectal Tumorigenesis.

Cancers

Chan YL,Lai WC,Chen JS,Tseng JT,Chuang PC,Jou J,Lee CT,Sun HS

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Wed Jul 08 00:00:00 EDT 2020

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

RIPK3 and Caspase-1/11 Are Necessary for Optimal Antigen-Specific CD8 T Cell Response Elicited by Genetically Modified Listeria monocytogenes.

Frontiers in immunology

Rana A,de Almeida FC,Paico Montero HA,Gonzales Carazas MM,Bortoluci KR,Sad S,Amarante-Mendes GP

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Fri Mar 26 00:00:00 EDT 2021

Artificial Mini Dendritic Cells Boost T Cell-Based Immunotherapy for Ovarian Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Cheng S,Xu C,Jin Y,Li Y,Zhong C,Ma J,Yang J,Zhang N,Li Y,Wang C,Yang Z,Wang Y

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Loss of testosterone impairs anti-tumor neutrophil function.

Nature communications

Markman JL,Porritt RA,Wakita D,Lane ME,Martinon D,Noval Rivas M,Luu M,Posadas EM,Crother TR,Arditi M

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Tue Mar 31 00:00:00 EDT 2020

Nuclear imaging-guided PD-L1 blockade therapy increases effectiveness of cancer immunotherapy.

Journal for immunotherapy of cancer

Gao H,Wu Y,Shi J,Zhang X,Liu T,Hu B,Jia B,Wan Y,Liu Z,Wang F

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Gasdermin D in macrophages restrains colitis by controlling cGAS-mediated inflammation.

Science advances

Ma C,Yang D,Wang B,Wu C,Wu Y,Li S,Liu X,Lassen K,Dai L,Yang S

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

ICOS agonism by JTX-2011 (vopratelimab) requires initial T cell priming and Fc cross-linking for optimal T cell activation and anti-tumor immunity in preclinical models.

PloS one

Hanson A,Elpek K,Duong E,Shallberg L,Fan M,Johnson C,Wallace M,Mabry GR,Sazinsky S,Pepper L,Shu CJ,Sathyanarayanan S,Zuerndorfer S,Simpson T,Gostissa M,Briskin M,Law D,Michaelson J,Harvey CJ

61-0081 was used in Flow cytometry/Cell sorting to describe the generation of specific, species cross-reactive, agonist antibodies to ICOS, including the humanized clinical candidate, JTX-2011 (vopratelimab).

Mon Nov 16 00:00:00 EST 2020

ICOS agonism by JTX-2011 (vopratelimab) requires initial T cell priming and Fc cross-linking for optimal T cell activation and anti-tumor immunity in preclinical models.

PloS one

Hanson A,Elpek K,Duong E,Shallberg L,Fan M,Johnson C,Wallace M,Mabry GR,Sazinsky S,Pepper L,Shu CJ,Sathyanarayanan S,Zuerndorfer S,Simpson T,Gostissa M,Briskin M,Law D,Michaelson J,Harvey CJ

Mon Nov 16 00:00:00 EST 2020

The PD-1 Pathway Regulates Development and Function of Memory CD8⁺ T Cells following Respiratory Viral Infection.

Cell reports

Pauken KE,Godec J,Odorizzi PM,Brown KE,Yates KB,Ngiow SF,Burke KP,Maleri S,Grande SM,Francisco LM,Ali MA,Imam S,Freeman GJ,Haining WN,Wherry EJ,Sharpe AH

61-0081-82 was used in Flow Cytometry to demonstrate that PD-1 signals are needed for optimal memory.

Tue Jun 30 00:00:00 EDT 2020

Specific targeting of IL-1β activity to CD8⁺ T cells allows for safe use as a vaccine adjuvant.

NPJ vaccines

Van Den Eeckhout B,Van Hoecke L,Burg E,Van Lint S,Peelman F,Kley N,Uzé G,Saelens X,Tavernier J,Gerlo S

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Tue Sep 19 00:00:00 EDT 2023

Antiallodynic Effects of Cannabinoid Receptor 2 (CB₂R) Agonists on Retrovirus Infection-Induced Neuropathic Pain.

Pain research & management

Sheng WS,Chauhan P,Hu S,Prasad S,Lokensgard JR

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Mon Dec 16 00:00:00 EST 2019

Amorphous silicon dioxide nanoparticles modulate immune responses in a model of allergic contact dermatitis.

Scientific reports

Palmer BC,Jatana S,Phelan-Dickinson SJ,DeLouise LA

61-0081 was used in Flow cytometry/Cell sorting to show amorphous silicon dioxide nanoparticles have an immunomodulatory effect in a model of allergic contact dermatitis.

Mon Mar 25 00:00:00 EDT 2019

STAT1 is a sex-specific tumor suppressor in colitis-associated colorectal cancer.

Molecular oncology

Crnčec I,Modak M,Gordziel C,Svinka J,Scharf I,Moritsch S,Pathria P,Schlederer M,Kenner L,Timelthaler G,Müller M,Strobl B,Casanova E,Bayer E,Mohr T,Stöckl J,Friedrich K,Eferl R

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Inhibiting TrxR suppresses liver cancer by inducing apoptosis and eliciting potent antitumor immunity.

Oncology reports

Lei H,Wang G,Zhang J,Han Q

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

Sipa1 deficiency unleashes a host-immune mechanism eradicating chronic myelogenous leukemia-initiating cells.

Nature communications

Xu Y,Ikeda S,Sumida K,Yamamoto R,Tanaka H,Minato N

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Fri Mar 02 00:00:00 EST 2018

A stochastic epigenetic switch controls the dynamics of T-cell lineage commitment.

eLife

Ng KK,Yui MA,Mehta A,Siu S,Irwin B,Pease S,Hirose S,Elowitz MB,Rothenberg EV,Kueh HY

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Tue Nov 20 00:00:00 EST 2018

Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus.

eLife

Moretti FA,Klapproth S,Ruppert R,Margraf A,Weber J,Pick R,Scheiermann C,Sperandio M,Fässler R,Moser M

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Thu Sep 06 00:00:00 EDT 2018

A protocol for generating induced T cells by reprogramming B cells in vivo.

Cell regeneration (London, England)

Weng Q,Hu F,Zhang M,Dong Y,Lv C,Wang Y,Liu X,Wang J

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Macrophage Depletion Protects against Cigarette Smoke-Induced Inflammatory Response in the Mouse Colon and Lung.

Frontiers in physiology

Lim D,Kim W,Lee C,Bae H,Kim J

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Loss of Nucleobindin-2 Causes Insulin Resistance in Obesity without Impacting Satiety or Adiposity.

Cell reports

Ravussin A,Youm YH,Sander J,Ryu S,Nguyen K,Varela L,Shulman GI,Sidorov S,Horvath TL,Schultze JL,Dixit VD

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Tue Jul 31 00:00:00 EDT 2018

Modifying the cancer-immune set point using vaccinia virus expressing re-designed interleukin-2.

Nature communications

Liu Z,Ge Y,Wang H,Ma C,Feist M,Ju S,Guo ZS,Bartlett DL

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Thu Nov 08 00:00:00 EST 2018

Microbiota Reconstitution Does Not Cause Bone Loss in Germ-Free Mice.

mSphere

Quach D,Collins F,Parameswaran N,McCabe L,Britton RA

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Differentiation and Protective Capacity of Virus-Specific CD8⁺ T Cells Suggest Murine Norovirus Persistence in an Immune-Privileged Enteric Niche.

Immunity

Tomov VT,Palko O,Lau CW,Pattekar A,Sun Y,Tacheva R,Bengsch B,Manne S,Cosma GL,Eisenlohr LC,Nice TJ,Virgin HW,Wherry EJ

61-0081 was used in Flow cytometry/Cell sorting to elucidate a strategy of immune evasion by mouse norovirus via the induction of a CD8+ T cell program normally reserved for latent pathogens and persistence in an immune-privileged enteric niche.

Tue Oct 17 00:00:00 EDT 2017

Adipose tissue B2 cells promote insulin resistance through leukotriene LTB4/LTB4R1 signaling.

The Journal of clinical investigation

Ying W,Wollam J,Ofrecio JM,Bandyopadhyay G,El Ouarrat D,Lee YS,Oh DY,Li P,Osborn O,Olefsky JM

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Combined antiangiogenic and anti-PD-L1 therapy stimulates tumor immunity through HEV formation.

Science translational medicine

Allen E,Jabouille A,Rivera LB,Lodewijckx I,Missiaen R,Steri V,Feyen K,Tawney J,Hanahan D,Michael IP,Bergers G

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Wed Apr 12 00:00:00 EDT 2017

Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation.

The Journal of experimental medicine

Miao T,Symonds ALJ,Singh R,Symonds JD,Ogbe A,Omodho B,Zhu B,Li S,Wang P

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Mon Jun 05 00:00:00 EDT 2017

A New Adjuvant MTOM Mediates Mycobacterium tuberculosis Subunit Vaccine to Enhance Th1-Type T Cell Immune Responses and IL-2⁺ T Cells.

Frontiers in immunology

Yu Q,Wang X,Fan X

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Betulin from Hedyotis hedyotidea ameliorates concanavalin A-induced and T cell-mediated autoimmune hepatitis in mice.

Acta pharmacologica Sinica

Zhou YQ,Weng XF,Dou R,Tan XS,Zhang TT,Fang JB,Wu XW

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

Role of Triggering Receptor Expressed on Myeloid Cell-1 Expression in Mammalian Target of Rapamycin Modulation of CD8⁺ T-cell Differentiation during the Immune Response to Invasive Pulmonary Aspergillosis.

Chinese medical journal

Cui N,Wang H,Su LX,Zhang JH,Long Y,Liu DW

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Sat May 20 00:00:00 EDT 2017

Immunological phenotype of the murine Lrba knockout.

Immunology and cell biology

Gámez-Díaz L,Neumann J,Jäger F,Proietti M,Felber F,Soulas-Sprauel P,Perruzza L,Grassi F,Kögl T,Aichele P,Kilimann M,Grimbacher B,Jung S

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Sun Oct 01 00:00:00 EDT 2017

ADAR1 deletion induces NFκB and interferon signaling dependent liver inflammation and fibrosis.

RNA biology

Ben-Shoshan SO,Kagan P,Sultan M,Barabash Z,Dor C,Jacob-Hirsch J,Harmelin A,Pappo O,Marcu-Malina V,Ben-Ari Z,Amariglio N,Rechavi G,Goldstein I,Safran M

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Thu May 04 00:00:00 EDT 2017

IL-2/Anti-IL-2 Complex Attenuates Inflammation and BBB Disruption in Mice Subjected to Traumatic Brain Injury.

Frontiers in neurology

Gao W,Li F,Zhou Z,Xu X,Wu Y,Zhou S,Yin D,Sun D,Xiong J,Jiang R,Zhang J

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Calreticulin is an effective immunologic adjuvant to tumor-associated antigens.

Experimental and therapeutic medicine

Wang J,Gao ZP,Qin S,Liu CB,Zou LL

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Sun Oct 01 00:00:00 EDT 2017

Transient Treg depletion enhances therapeutic anti-cancer vaccination.

Immunity, inflammation and disease

Fisher SA,Aston WJ,Chee J,Khong A,Cleaver AL,Solin JN,Ma S,Lesterhuis WJ,Dick I,Holt RA,Creaney J,Boon L,Robinson B,Lake RA

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Targeted calcium influx boosts cytotoxic T lymphocyte function in the tumour microenvironment.

Nature communications

Kim KD,Bae S,Capece T,Nedelkovska H,de Rubio RG,Smrcka AV,Jun CD,Jung W,Park B,Kim TI,Kim M

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Mon May 15 00:00:00 EDT 2017

Location of tumor affects local and distant immune cell type and number.

Immunity, inflammation and disease

Hensel JA,Khattar V,Ashton R,Lee C,Siegal GP,Ponnazhagan S

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting.

Scientific reports

Atkin-Smith GK,Paone S,Zanker DJ,Duan M,Phan TK,Chen W,Hulett MD,Poon IK

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Fri Jan 06 00:00:00 EST 2017

Identification of inhibitors of myeloid-derived suppressor cells activity through phenotypic chemical screening.

Oncoimmunology

Schröder M,Loos S,Naumann SK,Bachran C,Krötschel M,Umansky V,Helming L,Swee LK

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

IL-12 Influence mTOR to Modulate CD8⁺ T Cells Differentiation through T-bet and Eomesodermin in Response to Invasive Pulmonary Aspergillosis.

International journal of medical sciences

Wang H,Li J,Han Q,Yang F,Xiao Y,Xiao M,Xu Y,Su L,Cui N,Liu D

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Mon May 21 00:00:00 EDT 2018

Tristetraprolin inhibits macrophage IL-27-induced activation of antitumour cytotoxic T cell responses.

Nature communications

Wang Q,Ning H,Peng H,Wei L,Hou R,Hoft DF,Liu J

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Wed Oct 11 00:00:00 EDT 2017

IL-7-dependent STAT1 activation limits homeostatic CD4+ T cell expansion.

JCI insight

Le Saout C,Luckey MA,Villarino AV,Smith M,Hasley RB,Myers TG,Imamichi H,Park JH,O'Shea JJ,Lane HC,Catalfamo M

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Thu Nov 16 00:00:00 EST 2017

Potent single-domain antibodies that arrest respiratory syncytial virus fusion protein in its prefusion state.

Nature communications

Rossey I,Gilman MS,Kabeche SC,Sedeyn K,Wrapp D,Kanekiyo M,Chen M,Mas V,Spitaels J,Melero JA,Graham BS,Schepens B,McLellan JS,Saelens X

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Mon Feb 13 00:00:00 EST 2017

A Multistage Subunit Vaccine Effectively Protects Mice Against Primary Progressive Tuberculosis, Latency and Reactivation.

EBioMedicine

Ma J,Teng X,Wang X,Fan X,Wu Y,Tian M,Zhou Z,Li L

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Metabolic maintenance of cell asymmetry following division in activated T lymphocytes.

Nature

Verbist KC,Guy CS,Milasta S,Liedmann S,Kamiński MM,Wang R,Green DR

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Thu Apr 21 00:00:00 EDT 2016

The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity.

Cell

Damgaard RB,Walker JA,Marco-Casanova P,Morgan NV,Titheradge HL,Elliott PR,McHale D,Maher ER,McKenzie ANJ,Komander D

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Thu Aug 25 00:00:00 EDT 2016

Maternal-Derived Hepatitis B Virus e Antigen Alters Macrophage Function in Offspring to Drive Viral Persistence after Vertical Transmission.

Immunity

Tian Y,Kuo CF,Akbari O,Ou JH

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Tue May 17 00:00:00 EDT 2016

Oligodendrocyte death results in immune-mediated CNS demyelination.

Nature neuroscience

Traka M,Podojil JR,McCarthy DP,Miller SD,Popko B

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

Neutrophil Extracellular Traps Enhance Early Inflammatory Response in Sendai Virus-Induced Asthma Phenotype.

Frontiers in immunology

Akk A,Springer LE,Pham CT

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Mon Sep 12 00:00:00 EDT 2016

Opposite Effects of Coinjection and Distant Injection of Mesenchymal Stem Cells on Breast Tumor Cell Growth.

Stem cells translational medicine

Zheng H,Zou W,Shen J,Xu L,Wang S,Fu YX,Fan W

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

PRDM14 promotes RAG-dependent Notch1 driver mutations in mouse T-ALL.

Biology open

Carofino BL,Ayanga B,Tracey LJ,Brooke-Bisschop T,Justice MJ

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Sun May 15 00:00:00 EDT 2016

Stromal senescence establishes an immunosuppressive microenvironment that drives tumorigenesis.

Nature communications

Ruhland MK,Loza AJ,Capietto AH,Luo X,Knolhoff BL,Flanagan KC,Belt BA,Alspach E,Leahy K,Luo J,Schaffer A,Edwards JR,Longmore G,Faccio R,DeNardo DG,Stewart SA

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Wed Jun 08 00:00:00 EDT 2016

Epigallocatechin-3-gallate (EGCG) inhibits imiquimod-induced psoriasis-like inflammation of BALB/c mice.

BMC complementary and alternative medicine

Zhang S,Liu X,Mei L,Wang H,Fang F

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Wed Aug 31 00:00:00 EDT 2016

Discovery of a Metastatic Immune Escape Mechanism Initiated by the Loss of Expression of the Tumour Biomarker Interleukin-33.

Scientific reports

Saranchova I,Han J,Huang H,Fenninger F,Choi KB,Munro L,Pfeifer C,Welch I,Wyatt AW,Fazli L,Gleave ME,Jefferies WA

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Tue Sep 13 00:00:00 EDT 2016

Exploring the induction of preproinsulin-specific Foxp3(+) CD4(+) Treg cells that inhibit CD8(+) T cell-mediated autoimmune diabetes by DNA vaccination.

Scientific reports

Stifter K,Schuster C,Schlosser M,Boehm BO,Schirmbeck R

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Mon Jul 11 00:00:00 EDT 2016

Photothermal therapy with immune-adjuvant nanoparticles together with checkpoint blockade for effective cancer immunotherapy.

Nature communications

Chen Q,Xu L,Liang C,Wang C,Peng R,Liu Z

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Fri Oct 21 00:00:00 EDT 2016

Injectable, spontaneously assembling, inorganic scaffolds modulate immune cells in vivo and increase vaccine efficacy.

Nature biotechnology

Kim J,Li WA,Choi Y,Lewin SA,Verbeke CS,Dranoff G,Mooney DJ

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

FOXP3+ regulatory T cell development and function require histone/protein deacetylase 3.

The Journal of clinical investigation

Wang L,Liu Y,Han R,Beier UH,Bhatti TR,Akimova T,Greene MI,Hiebert SW,Hancock WW

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2015

Atypical chemokine receptor 1 deficiency reduces atherogenesis in ApoE-knockout mice.

Cardiovascular research

Wan W,Liu Q,Lionakis MS,Marino AP,Anderson SA,Swamydas M,Murphy PM

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

MHC-mismatched mixed chimerism augments thymic regulatory T-cell production and prevents relapse of EAE in mice.

Proceedings of the National Academy of Sciences of the United States of America

Wu L,Li N,Zhang M,Xue SL,Cassady K,Lin Q,Riggs AD,Zeng D

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Tue Dec 29 00:00:00 EST 2015

CD31 signals confer immune privilege to the vascular endothelium.

Proceedings of the National Academy of Sciences of the United States of America

Cheung K,Ma L,Wang G,Coe D,Ferro R,Falasca M,Buckley CD,Mauro C,Marelli-Berg FM

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Tue Oct 27 00:00:00 EDT 2015

Single-Dose CpG Immunization Protects Against a Heterosubtypic Challenge and Generates Antigen-Specific Memory T Cells.

Frontiers in immunology

Vogel AJ,Brown DM

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Fri Jul 10 00:00:00 EDT 2015

A tumor lysate is an effective vaccine antigen for the stimulation of CD4(+) T-cell function and subsequent induction of antitumor immunity mediated by CD8(+) T cells.

Cancer biology & therapy

Kawahara M,Takaku H

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Mon Sep 12 00:00:00 EDT 2016

Adjuvants may reduce in vivo transfection levels for DNA vaccination in mice leading to reduced antigen-specific CD8+ T cell responses.

Human vaccines & immunotherapeutics

Rose AH,Hoffmann FW,Hara JH,Urschitz J,Moisyadi S,Hoffmann PR,Bertino P

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Mon May 16 00:00:00 EDT 2016

Immunogenicity and protective efficacy of DMT liposome-adjuvanted tuberculosis subunit CTT3H vaccine.

Human vaccines & immunotherapeutics

Teng X,Tian M,Li J,Tan S,Yuan X,Yu Q,Jing Y,Zhang Z,Yue T,Zhou L,Fan X

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Fri Mar 11 00:00:00 EST 2016

Favorable alteration of tumor microenvironment by immunomodulatory cytokines for efficient T-cell therapy in solid tumors.

PloS one

Tähtinen S,Kaikkonen S,Merisalo-Soikkeli M,Grönberg-Vähä-Koskela S,Kanerva A,Parviainen S,Vähä-Koskela M,Hemminki A

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Thu Apr 28 00:00:00 EDT 2016

MFG-E8 Is Critical for Embryonic Stem Cell-Mediated T Cell Immunomodulation.

Stem cell reports

Tan Y,AlKhamees B,Jia D,Li L,Couture JF,Figeys D,Jinushi M,Wang L

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Tue Nov 10 00:00:00 EST 2015

Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation.

eLife

He L,Zhang Y,Ma G,Tan P,Li Z,Zang S,Wu X,Jing J,Fang S,Zhou L,Wang Y,Huang Y,Hogan PG,Han G,Zhou Y

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Tue Dec 08 00:00:00 EST 2015

A new animal model of spontaneous autoimmune peripheral polyneuropathy: implications for Guillain-Barré syndrome.

Acta neuropathologica communications

Yang M,Rainone A,Shi XQ,Fournier S,Zhang J

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Wed Jan 08 00:00:00 EST 2014

Deficiency of autophagy in dendritic cells protects against experimental autoimmune encephalomyelitis.

The Journal of biological chemistry

Bhattacharya A,Parillon X,Zeng S,Han S,Eissa NT

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Fri Sep 19 00:00:00 EDT 2014

Mutual expression of the transcription factors Runx3 and ThPOK regulates intestinal CD4⁺ T cell immunity.

Nature immunology

Reis BS,Rogoz A,Costa-Pinto FA,Taniuchi I,Mucida D

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

Dose-dense chemotherapy improves mechanisms of antitumor immune response.

Cancer research

Chang CL,Hsu YT,Wu CC,Lai YZ,Wang C,Yang YC,Wu TC,Hung CF

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2013

Genetic deletion of chemokine receptor Ccr7 exacerbates atherogenesis in ApoE-deficient mice.

Cardiovascular research

Wan W,Lionakis MS,Liu Q,Roffê E,Murphy PM

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

A mouse model for inducible overexpression of Prdm14 results in rapid-onset and highly penetrant T-cell acute lymphoblastic leukemia (T-ALL).

Disease models & mechanisms

Carofino BL,Ayanga B,Justice MJ

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2013

Interleukin-22 as a molecular adjuvant facilitates IL-17-producing CD8+ T cell responses against a HBV DNA vaccine in mice.

Human vaccines & immunotherapeutics

Wu B,Zou Q,Hu Y,Wang B

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2013

TDP2-dependent non-homologous end-joining protects against topoisomerase II-induced DNA breaks and genome instability in cells and in vivo.

PLoS genetics

Gómez-Herreros F,Romero-Granados R,Zeng Z,Alvarez-Quilón A,Quintero C,Ju L,Umans L,Vermeire L,Huylebroeck D,Caldecott KW,Cortés-Ledesma F

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Thu Jun 13 00:00:00 EDT 2013

In vivo CD8+ T cell dynamics in the liver of Plasmodium yoelii immunized and infected mice.

PloS one

Cabrera M,Pewe LL,Harty JT,Frevert U

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Tue Mar 18 00:00:00 EDT 2014

A missing PD-L1/PD-1 coinhibition regulates diabetes induction by preproinsulin-specific CD8 T-cells in an epitope-specific manner.

PloS one

Schuster C,Brosi H,Stifter K,Boehm BO,Schirmbeck R

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Tue Apr 08 00:00:00 EDT 2014

Absence of Wip1 partially rescues Atm deficiency phenotypes in mice.

Oncogene

Darlington Y,Nguyen TA,Moon SH,Herron A,Rao P,Zhu C,Lu X,Donehower LA

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2012

Evaluation of Lassa virus vaccine immunogenicity in a CBA/J-ML29 mouse model.

Vaccine

Goicochea MA,Zapata JC,Bryant J,Davis H,Salvato MS,Lukashevich IS

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Tue Feb 14 00:00:00 EST 2012

Characterization of transgenic mice expressing cancer-associated variants of human NOTCH1.

Genesis (New York, N.Y. : 2000)

Berquam-Vrieze KE,Swing DA,Tessarollo L,Dupuy AJ

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2012

Sensitized phenotypic screening identifies gene dosage sensitive region on chromosome 11 that predisposes to disease in mice.

EMBO molecular medicine

Ermakova O,Piszczek L,Luciani L,Cavalli FM,Ferreira T,Farley D,Rizzo S,Paolicelli RC,Al-Banchaabouchi M,Nerlov C,Moriggl R,Luscombe NM,Gross C

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Sat Jan 01 00:00:00 EST 2011

T cell factor 1 regulates thymocyte survival via a RORγt-dependent pathway.

Journal of immunology (Baltimore, Md. : 1950)

Wang R,Xie H,Huang Z,Ma J,Fang X,Ding Y,Sun Z

Published figure using CD8a monoclonal antibody (Product # 61-0081-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2011

Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2.

Investigative ophthalmology & visual science

Mochimaru H,Usui T,Yaguchi T,Nagahama Y,Hasegawa G,Usui Y,Shimmura S,Tsubota K,Amano S,Kawakami Y,Ishida S

Thu May 01 00:00:00 EDT 2008

Myocardial infarct-sparing effect of adenosine A2A receptor activation is due to its action on CD4+ T lymphocytes.

Circulation

Yang Z,Day YJ,Toufektsian MC,Xu Y,Ramos SI,Marshall MA,French BA,Linden J

Tue Nov 07 00:00:00 EST 2006

Bioinformatics

Protein Aliases: CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Lymphocyte antigen 3; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-cell surface glycoprotein Lyt-3

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Gene Aliases: BB154331; Cd8a; Cd8b; Cd8b1; Ly-2; Ly-3; Ly-35; Ly-B; Ly-C; Lyt-2; Lyt-3; Lyt2; Lyt3

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UniProt ID: (Mouse) P01731, (Mouse) P10300

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Entrez Gene ID: (Mouse) 12525, (Mouse) 12526

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Function(s)

protein binding protein kinase binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

Process(es)

immune system process T cell mediated immunity cell surface receptor signaling pathway T cell activation cytotoxic T cell differentiation positive regulation of calcium-mediated signaling defense response to virus

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CD8a Monoclonal Antibody (53-6.7), PE-eFluor™ 610, eBioscience™

CD8a Antibody (61-0081-82) in Flow

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61-0081-82

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