Invitrogen

CD8a Monoclonal Antibody (53-6.7), PE-Cyanine7, eBioscience™

FIGURE: 1 / 1

CD8a Antibody (25-0081-82) in Flow

Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.25 µg of Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (left) or 0.25 µg of Anti-Mouse CD8a PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

25-0081-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 175 publications 175 publications

Functional Assay (Functional)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

53-6.7

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469584

Product Specific Information

Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.

Applications Reported: The 53-6.7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 53-6.7 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Product References

Pexidartinib synergize PD-1 antibody through inhibiting treg infiltration by reducing TAM-derived CCL22 in lung adenocarcinoma.

Frontiers in pharmacology

Zhang W,Jiang X,Zou Y,Yuan L,Wang X

25-0081-82 was used in Flow Cytometry to show that the combination of pexidartinib and PD-1 antibody showed a synergy and significantly improved the anti-tumor efficacy, through pexidartinib increasing CD8T/Treg ratio by reducing TAM-derived CCL22.

Tue Mar 28 00:00:00 EDT 2023

MCL-1 Inhibitor S63845 Distinctively Affects Intramedullary and Extramedullary Hematopoiesis.

Pharmaceutics

Zhang H,Li F,Yang M,Zhang W,He M,Xu H,Wang C,Zhang Y,Wang W,Gao Y,Du X,Li Y

25-0081-82 was used in Flow cytometry/Cell sorting to demonstrate the effects of myeloid cell leukemia 1 (MCL-1) inhibitor on the intramedullary and extramedullary hematopoietic lineages, which is important for the selection of combinations of antitumor drugs and the prevention of adverse hematopoiesis-related effects.

Tue Mar 28 00:00:00 EDT 2023

Anti-tumor memory CD4 and CD8 T-cells quantified by bulk T-cell receptor (TCR) clonal analysis.

Frontiers in immunology

Gao Y,Bergman I

25-0081-82 was used in Flow cytometry/Cell sorting to show that this methodology can be developed to use blood and tissue sampling to rapidly quantify the effectiveness of a tumor vaccine or any vaccine generating therapeutic T-cells.

Tue Apr 11 00:00:00 EDT 2023

Histone methyltransferase WHSC1 loss dampens MHC-I antigen presentation pathway to impair IFN-γ-stimulated antitumor immunity.

The Journal of clinical investigation

Ren J,Li N,Pei S,Lian Y,Li L,Peng Y,Liu Q,Guo J,Wang X,Han Y,Zhang G,Wang H,Li Y,Jiang J,Li Q,Tan M,Peng J,Hu G,Xiao Y,Li X,Lin M,Qin J

25-0081-82 was used in Flow Cytometry to establish a tumor-suppressive function of WHSC1 that relays IFN-γ signaling to promote antigen presentation in CRC cells, and provide a rationale for boosting WHSC1 activity in immunotherapy.

Fri Apr 15 00:00:00 EDT 2022

Universal protection against influenza viruses by multi-subtype neuraminidase and M2 ectodomain virus-like particle.

PLoS pathogens

Kim KH,Li Z,Bhatnagar N,Subbiah J,Park BR,Shin CH,Pushko P,Wang BZ,Kang SM

25-0081-82 was used in Flow cytometry/Cell sorting to support a novel strategy of developing a universal vaccine against influenza A and B viruses potentially in both young and aged populations by inducing multi-NA subtype and M2e immunity with a single VLP entity.

Mon Aug 01 00:00:00 EDT 2022

Abdominopelvic FLASH Irradiation Improves PD-1 Immune Checkpoint Inhibition in Preclinical Models of Ovarian Cancer.

Molecular cancer therapeutics

Eggold JT,Chow S,Melemenidis S,Wang J,Natarajan S,Loo PE,Manjappa R,Viswanathan V,Kidd EA,Engleman E,Dorigo O,Loo BW,Rankin EB

25-0081-82 was used in Flow cytometry/Cell sorting to highlight the potential for FLASH irradiation to improve the therapeutic efficacy of checkpoint inhibition in the treatment of ovarian cancer.

Tue Feb 01 00:00:00 EST 2022

Meningeal lymphatic drainage promotes T cell responses against Toxoplasma gondii but is dispensable for parasite control in the brain.

eLife

Kovacs MA,Cowan MN,Babcock IW,Sibley LA,Still K,Batista SJ,Labuzan SA,Sethi I,Harris TH

25-0081-82 was used in Flow cytometry/Cell sorting to reveal that CNS lymphatic drainage supports the development of peripheral T cell responses against T. gondii but remains dispensable for immune protection of the brain.

Wed Dec 21 00:00:00 EST 2022

The parasitic worm product ES-62 protects the osteoimmunology axis in a mouse model of obesity-accelerated ageing.

Frontiers in immunology

Harnett MM,Doonan J,Lumb FE,Crowe J,Damink RO,Buitrago G,Duncombe-Moore J,Wilkinson DI,Suckling CJ,Selman C,Harnett W

25-0081-82 was used in Flow cytometry/Cell sorting to report the ability of small drug-like molecule analogues of ES-62 to mimic some of its key actions, particularly in strongly protecting trabecular bone structure, highlighting the translational potential of these studies.

Fri Sep 16 00:00:00 EDT 2022

Maternal-fetal conflict averted by progesterone- induced FOXP3+ regulatory T cells.

iScience

Severance AL,Kinder JM,Xin L,Burg AR,Shao TY,Pham G,Tilburgs T,Goodman WA,Mesiano S,Way SS

25-0081-82 was used in Flow cytometry/Cell sorting to show that the female reproductive hormone, progesterone, coordinates immune tolerance by stimulating expansion of FOXP3+ regulatory T cells.

Fri Jun 17 00:00:00 EDT 2022

Cancer cells dying from ferroptosis impede dendritic cell-mediated anti-tumor immunity.

Nature communications

Wiernicki B,Maschalidi S,Pinney J,Adjemian S,Vanden Berghe T,Ravichandran KS,Vandenabeele P

25-0081-82 was used in Flow cytometry/Cell sorting to show that ferroptosis negatively impacts antigen presenting cells and hence the adaptive immune response, which might hinder therapeutic applications of ferroptosis induction.

Mon Jun 27 00:00:00 EDT 2022

The secreted protein Cowpox Virus 14 contributes to viral virulence and immune evasion by engaging Fc-gamma-receptors.

PLoS pathogens

Iyer RF,Edwards DM,Kolb P,Raué HP,Nelson CA,Epperson ML,Slifka MK,Nolz JC,Hengel H,Fremont DH,Früh K

25-0081-82 was used in flow cytometry experiments to suggest that CPXV14 contributes to virulence and immune evasion by binding to host FcγRs.

Thu Sep 01 00:00:00 EDT 2022

Mannan-Binding Lectin Promotes Murine Graft-versus-Host Disease by Amplifying Lipopolysaccharide-Initiated Inflammation.

Transplantation and cellular therapy

Heja D,Zhao D,Cody E,Cumpelik A,Lim PC,Prado-Acosta M,Palma L,Dellepiane S,Chun N,Ferrara J,Heeger PS

25-0081-82 was used in Flow cytometry/Cell sorting to suggest that devising strategies to block LPS/MBL ligation on APCs has the potential to reduce the clinical expression of GVHD.

Mon Aug 01 00:00:00 EDT 2022

Pannexin 1 channels facilitate communication between T cells to restrict the severity of airway inflammation.

Immunity

Medina CB,Chiu YH,Stremska ME,Lucas CD,Poon I,Tung KS,Elliott MR,Desai B,Lorenz UM,Bayliss DA,Ravichandran KS

25-0081-82 was used in Flow cytometry/Cell sorting to uncover an immunoregulatory role for the nucleotide release channel, Panx1, in T cell crosstalk during airway disease.

Tue Aug 10 00:00:00 EDT 2021

Modulation of lactate-lysosome axis in dendritic cells by clotrimazole potentiates antitumor immunity.

Journal for immunotherapy of cancer

Wang Z,Xu F,Hu J,Zhang H,Cui L,Lu W,He W,Wang X,Li M,Zhang H,Xiong W,Xie C,Liu Y,Zhou P,Liu J,Huang P,Qin XF,Xia X

25-0081-82 was used in Flow Cytometry to show that clotrimazole could trigger DC activation via the lactate-lysosome axis to promote antigen cross-presentation and could be used as a potential combination therapy approach to improving the therapeutic efficacy of anti-PD1 immunotherapy.

Sat May 01 00:00:00 EDT 2021

USP12 downregulation orchestrates a protumourigenic microenvironment and enhances lung tumour resistance to PD-1 blockade.

Nature communications

Yang Z,Xu G,Wang B,Liu Y,Zhang L,Jing T,Tang M,Xu X,Jiao K,Xiang L,Fu Y,Tang D,Zhang X,Jin W,Zhuang G,Zhao X,Liu Y

25-0081-82 was used in Flow Cytometry to show that the deubiquitinase USP12 is commonly downregulated in the KrasG12D-driven mouse lung tumour and human non-small cell lung cancer owing to the activation of AKT-mTOR signalling.

Wed Aug 11 00:00:00 EDT 2021

Robust induction of T_RMs by combinatorial nanoshells confers cross-strain sterilizing immunity against lethal influenza viruses.

Molecular therapy. Methods & clinical development

Lin PH,Liang CY,Yao BY,Chen HW,Pan CF,Wu LL,Lin YH,Hsu YS,Liu YH,Chen PJ,Hu CJ,Yang HC

25-0081-82 was used in Flow Cytometry to demonstrate safe and potent induction of lung-resident memory T cells in a biodegradable, virus-mimicking structure.

Fri Jun 11 00:00:00 EDT 2021

G-protein-coupled receptor P2Y10 facilitates chemokine-induced CD4 T cell migration through autocrine/paracrine mediators.

Nature communications

Gurusamy M,Tischner D,Shao J,Klatt S,Zukunft S,Bonnavion R,Günther S,Siebenbrodt K,Kestner RI,Kuhlmann T,Fleming I,Offermanns S,Wettschureck N

25-0081-82 was used in Flow cytometry/Cell sorting to show that in mice lacking P2Y10 in the CD4 T cell compartment, the severity of experimental autoimmune encephalomyelitis and cutaneous contact hypersensitivity is reduced.

Tue Nov 23 00:00:00 EST 2021

Immune-tolerance to human iPS-derived neural progenitors xenografted into the immature cerebellum is overridden by species-specific differences in differentiation timing.

Scientific reports

Nato G,Corti A,Parmigiani E,Jachetti E,Lecis D,Colombo MP,Delia D,Buffo A,Magrassi L

25-0081-82 was used in Flow Cytometry to monitor xeno-transplanted human neural precursor cells derived from induced pluripotent stem cells into the cerebellum and brainstem of mice and rats during prenatal development or the first postnatal week.

Tue Jan 12 00:00:00 EST 2021

BCG Cell Wall Skeleton As a Vaccine Adjuvant Protects Both Infant and Old-Aged Mice from Influenza Virus Infection.

Biomedicines

Kim KH,Lee YT,Park Y,Ko EJ,Jung YJ,Kim YJ,Jo EK,Kang SM

25-0081-82 was used in Flow Cytometry to investigate the roles of Bacillus Calmette-Guerin (BCG) and the cell wall skeleton (CWS) derived from BCG as vaccine adjuvants.

Wed May 05 00:00:00 EDT 2021

Analysis of T cells in mouse lymphoid tissue and blood with flow cytometry.

STAR protocols

Skordos I,Demeyer A,Beyaert R

25-0081-82 was used in Flow Cytometry to provide a protocol for analysis of T cell activation and cytokine production in the thymus, spleen, and blood.

Fri Mar 19 00:00:00 EDT 2021

Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution.

Nature communications

Lima LG,Ham S,Shin H,Chai EPZ,Lek ESH,Lobb RJ,Müller AF,Mathivanan S,Yeo B,Choi Y,Parker BS,Möller A

25-0081-82 was used in Flow Cytometry to show that microenvironmental cytokines, particularly CCL2, decorate cancer exosomes via binding to surface glycosaminoglycan side chains of proteoglycans, causing exosome accumulation in specific cell subsets and organs.

Thu Jun 10 00:00:00 EDT 2021

Ontogenic timing, T cell receptor signal strength, and Notch signaling direct γδ T cell functional differentiation in vivo.

Cell reports

Chen ELY,Lee CR,Thompson PK,Wiest DL,Anderson MK,Zúñiga-Pflücker JC

25-0081-82 was used in Flow Cytometry to employ RBPJ-inducible and KN6-transgenic mice to assess the roles of ontogenic timing, T cell receptor (TCR) signal strength, and Notch signaling.

Tue Jun 08 00:00:00 EDT 2021

Graded RhoA GTPase Expression in Treg Cells Distinguishes Tumor Immunity From Autoimmunity.

Frontiers in immunology

Kalim KW,Yang JQ,Modur V,Nguyen P,Li Y,Zheng Y,Guo F

25-0081-82 was used in Flow cytometry/Cell sorting to suggest that graded RhoA expression in Treg cells distinguishes tumor immunity from autoimmunity and that rational targeting of RhoA in Treg cells may trigger anti-tumor T cell immunity without causing autoimmune responses.

Tue Jan 11 00:00:00 EST 2022

Remote Limb Ischemic Postconditioning Protects Against Ischemic Stroke by Promoting Regulatory T Cells Thriving.

Journal of the American Heart Association

Yu HH,Ma XT,Ma X,Chen M,Chu YH,Wu LJ,Wang W,Qin C,Tian DS

25-0081-82 was used in Flow Cytometry to demonstrate that Remote Limb Ischemic Postconditioning protects against ischemic brain injury, at least in part by activating and maintaining the Tregs through the nicotinamide adenine dinucleotide/nicotinamide adenine dinucleotide hydrate pathway.

Tue Nov 16 00:00:00 EST 2021

Interleukin-17A Serves a Priming Role in Autoimmunity by Recruiting IL-1β-Producing Myeloid Cells that Promote Pathogenic T Cells.

Immunity

McGinley AM,Sutton CE,Edwards SC,Leane CM,DeCourcey J,Teijeiro A,Hamilton JA,Boon L,Djouder N,Mills KHG

25-0081 was used in Flow cytometry/Cell sorting to demonstrate that a key function of IL-17A in central nervous system (CNS) autoimmunity is to recruit IL-1β-secreting myeloid cells that prime pathogenic γδT17 and Th17 cells.

Tue Feb 18 00:00:00 EST 2020

STAT3 Activation-Induced Fatty Acid Oxidation in CD8⁺ T Effector Cells Is Critical for Obesity-Promoted Breast Tumor Growth.

Cell metabolism

Zhang C,Yue C,Herrmann A,Song J,Egelston C,Wang T,Zhang Z,Li W,Lee H,Aftabizadeh M,Li YJ,Lee PP,Forman S,Somlo G,Chu P,Kruper L,Mortimer J,Hoon DSB,Huang W,Priceman S,Yu H

25-0081 was used in Flow cytometry/Cell sorting to show that increased fatty acid oxidation (FAO) driven by activated STAT3 in CD8+ T effector cells is critical for obesity-associated breast tumor progression.

Tue Jan 07 00:00:00 EST 2020

Nucleoside-modified mRNA vaccination partially overcomes maternal antibody inhibition of de novo immune responses in mice.

Science translational medicine

Willis E,Pardi N,Parkhouse K,Mui BL,Tam YK,Weissman D,Hensley SE

25-0081-82 was used in Flow Cytometry to demonstrate that influenza virus-specific maternal antibodies inhibited de novo antibody responses in mouse pups elicited by influenza virus infection or administration of conventional influenza vaccines.

Wed Jan 08 00:00:00 EST 2020

Snai2 Maintains Bone Marrow Niche Cells by Repressing Osteopontin Expression.

Developmental cell

Wei Q,Nakahara F,Asada N,Zhang D,Gao X,Xu C,Alfieri A,Brodin NP,Zimmerman SE,Mar JC,Guha C,Guo W,Frenette PS

25-0081-82 was used in Flow Cytometry to conclude that SNAI2 is a critical regulator of the transcriptional network maintaining MSPCs by the suppression of osteopontin expression.

Mon Jun 08 00:00:00 EDT 2020

Notch dimerization and gene dosage are important for normal heart development, intestinal stem cell maintenance, and splenic marginal zone B-cell homeostasis during mite infestation.

PLoS biology

Kobia FM,Preusse K,Dai Q,Weaver N,Hass MR,Chaturvedi P,Stein SJ,Pear WS,Yuan Z,Kovall RA,Kuang Y,Eafergen N,Sprinzak D,Gebelein B,Brunskill EW,Kopan R

25-0081 was used in Flow cytometry/Cell sorting to highlight the role of the environment in malignancy and colitis and is consistent with Notch-dependent anti-parasite immune responses being compromised in Notch dimer-deficient animals.

Thu Oct 01 00:00:00 EDT 2020

The parasitic worm product ES-62 promotes health- and life-span in a high calorie diet-accelerated mouse model of ageing.

PLoS pathogens

Crowe J,Lumb FE,Doonan J,Broussard M,Tarafdar A,Pineda MA,Landabaso C,Mulvey L,Hoskisson PA,Babayan SA,Selman C,Harnett W,Harnett MM

25-0081-82 was used in Flow Cytometry to investigate the potential of ES-62, an anti-inflammatory secreted product of the filarial nematode Acanthocheilonema viteae, to improve healthspan (the period of life before diseases of ageing appear) by targeting the chronic inflammation that drives metabolic dysregulation underpinning ageing-induced ill-health.

Sun Mar 01 00:00:00 EST 2020

DKK2 blockage-mediated immunotherapy enhances anti-angiogenic therapy of Kras mutated colorectal cancer.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Hu J,Wang Z,Chen Z,Li A,Sun J,Zheng M,Wu J,Shen T,Qiao J,Li L,Li B,Wu D,Xiao Q

25-0081-82 was used in Flow Cytometry to demonstrate that if in human colorectal cancer tumor samples expressing high levels of DKK2, DKK2 blockade cause stronger activation of tumor infiltrating CD8+ T cells in ex vivo culture.

Wed Jul 01 00:00:00 EDT 2020

MOSPD2 is a therapeutic target for the treatment of CNS inflammation.

Clinical and experimental immunology

Yacov N,Kafri P,Salem Y,Propheta-Meiran O,Feldman B,Breitbart E,Mendel I

25-0081 was used in Flow cytometry/Cell sorting to study the therapeutic potential of MOSPD2 in central nervous system disease.

Sat Aug 01 00:00:00 EDT 2020

Dietary Choline Supplementation Attenuates High-Fat-Diet-Induced Hepatocellular Carcinoma in Mice.

The Journal of nutrition

Brown AL,Conrad K,Allende DS,Gromovsky AD,Zhang R,Neumann CK,Owens AP,Tranter M,Helsley RN

25-0081-82 was used in Flow Cytometry to study whether choline supplementation could influence the progression of hepatocellular carcinoma in a high-fat-diet-driven mouse model.

Wed Apr 01 00:00:00 EDT 2020

Immunotherapeutic Blockade of CD47 Inhibitory Signaling Enhances Innate and Adaptive Immune Responses to Viral Infection.

Cell reports

Cham LB,Torrez Dulgeroff LB,Tal MC,Adomati T,Li F,Bhat H,Huang A,Lang PA,Moreno ME,Rivera JM,Galkina SA,Kosikova G,Stoddart CA,McCune JM,Myers LM,Weissman IL,Lang KS,Hasenkrug KJ

25-0081 was used in Flow cytometry/Cell sorting to indicate that CD47 blockade not only enhances the function of innate immune cells but also links to adaptive immune responses through improved APC function.

Tue Apr 14 00:00:00 EDT 2020

The Opposing Effect of Type I IFN on the T Cell Response by Non-modified mRNA-Lipoplex Vaccines Is Determined by the Route of Administration.

Molecular therapy. Nucleic acids

Van Hoecke L,Roose K,Ballegeer M,Zhong Z,Sanders NN,De Koker S,Saelens X,Van Lint S

25-0081-82 was used in Flow cytometry/Cell sorting to demonstrate that the opposing roles of type I type I interferon signaling on the magnitude of the vaccine-evoked T cell responses is dependent on the route of mRNA-lipoplex administration and is regulated at the level of the T cells rather than indirectly through modulation of dendritic cell function.

Fri Dec 04 00:00:00 EST 2020

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

25-0081 was used in Flow cytometry/Cell sorting to show that disruption of the Gdpd3 gene encoding a lysophospholipase D enzyme decreases the self-renewal capacity of murine chronic myelogenous leukaemia stem cells.

Thu Sep 17 00:00:00 EDT 2020

CD8⁺ T Cell Functional Exhaustion Overrides Pregnancy-Induced Fetal Antigen Alloimmunization.

Cell reports

Kinder JM,Turner LH,Stelzer IA,Miller-Handley H,Burg A,Shao TY,Pham G,Way SS

25-0081-82 was used in Flow Cytometry to demonstrate distinct mechanisms whereby fetal tolerance is maintained during primary compared with subsequent pregnancies.

Tue Jun 23 00:00:00 EDT 2020

Donor bone-marrow CXCR4+ Foxp3+ T-regulatory cells are essential for costimulation blockade-induced long-term survival of murine limb transplants.

Scientific reports

Wang L,Wang Z,Han R,Samanta A,Ge G,Levin LS,Levine MH,Hancock WW

25-0081 was used in Flow cytometry/Cell sorting to tested whether peri-transplant costimulation blockade could prolong VCA survival and required donor bone-marrow cells, given that bone-marrow might promote graft immunogenicity or graft-versus-host disease.

Tue Jun 09 00:00:00 EDT 2020

Bendamustine with total body irradiation conditioning yields tolerant T-cells while preserving T-cell-dependent graft-versus-leukemia.

Oncoimmunology

Stokes J,Hoffman EA,Molina MS,Kummet N,Simpson RJ,Zeng Y,Katsanis E

25-0081 was used in Flow cytometry/Cell sorting to demonstrate that BEN-TBI reduces graft-versus-host disease (GvHD) compared to CY-TBI independently of T regulatory cells (Tregs).

Wed Jul 14 00:00:00 EDT 2021

TGFβ-blockade uncovers stromal plasticity in tumors by revealing the existence of a subset of interferon-licensed fibroblasts.

Nature communications

Grauel AL,Nguyen B,Ruddy D,Laszewski T,Schwartz S,Chang J,Chen J,Piquet M,Pelletier M,Yan Z,Kirkpatrick ND,Wu J,deWeck A,Riester M,Hims M,Geyer FC,Wagner J,MacIsaac K,Deeds J,Diwanji R,Jayaraman P,Yu Y,Simmons Q,Weng S,Raza A,Minie B,Dostalek M,Chikkegowda P,Ruda V,Iartchouk O,Chen N,Thierry R,Zhou J,Pruteanu-Malinici I,Fabre C,Engelman JA,Dranoff G,Cremasco V

25-0081 was used in Flow cytometry/Cell sorting to perform an unbiased interrogation of tumor mesenchymal cells, delineating the co-existence of distinct subsets of cancer-associated fibroblasts (CAFs) in the microenvironment of murine carcinomas, each endowed with unique phenotypic features and functions.

Wed Dec 09 00:00:00 EST 2020

Genome-wide analysis highlights contribution of immune system pathways to the genetic architecture of asthma.

Nature communications

Han Y,Jia Q,Jahani PS,Hurrell BP,Pan C,Huang P,Gukasyan J,Woodward NC,Eskin E,Gilliland FD,Akbari O,Hartiala JA,Allayee H

25-0081 was used in Flow cytometry/Cell sorting to elucidate the genetic architecture of asthma and provide important insight into the immunological and sex-specific relevance of asthma-associated risk variants.

Wed Apr 15 00:00:00 EDT 2020

Lymphocyte-Specific Function of the DNA Polymerase Epsilon Subunit Pole3 Revealed by Neomorphic Alleles.

Cell reports

Siamishi I,Iwanami N,Clapes T,Trompouki E,O'Meara CP,Boehm T

25-0081 was used in Flow cytometry/Cell sorting to serve as a paradigm to understand the molecular basis of cell-type-specific non-replicative functions of the ubiquitous POLE complex.

Tue Jun 16 00:00:00 EDT 2020

Mucosal-associated invariant T cells promote inflammation and intestinal dysbiosis leading to metabolic dysfunction during obesity.

Nature communications

Toubal A,Kiaf B,Beaudoin L,Cagninacci L,Rhimi M,Fruchet B,da Silva J,Corbett AJ,Simoni Y,Lantz O,Rossjohn J,McCluskey J,Lesnik P,Maguin E,Lehuen A

25-0081 was used in Flow cytometry/Cell sorting to show that during obesity MAIT cells promote inflammation in both adipose tissue and ileum, leading to insulin resistance and impaired glucose and lipid metabolism.

Fri Jul 24 00:00:00 EDT 2020

Cancer-Specific Loss of p53 Leads to a Modulation of Myeloid and T Cell Responses.

Cell reports

Blagih J,Zani F,Chakravarty P,Hennequart M,Pilley S,Hobor S,Hock AK,Walton JB,Morton JP,Gronroos E,Mason S,Yang M,McNeish I,Swanton C,Blyth K,Vousden KH

25-0081 was used in Flow cytometry/Cell sorting to show suppressive regulatory T cells accumulate in tumour cells in the absence of p53 indicating a pathway in which cancer cells circumvent immune destruction.

Tue Jan 14 00:00:00 EST 2020

Axenic Caenorhabditis elegans antigen protects against development of type-1 diabetes in NOD mice.

Journal of translational autoimmunity

Jackson-Thompson BM,Torrero M,Mitre BK,Long J,Packiam M,Mitre E

25-0081-82 was used in Flow Cytometry to investigate whether soluble antigens obtained from Caenorhabditis elegans can confer protection against inflammatory disease.

Mon Sep 28 00:00:00 EDT 2020

Recirculating Intestinal IgA-Producing Cells Regulate Neuroinflammation via IL-10.

Cell

Rojas OL,Pröbstel AK,Porfilio EA,Wang AA,Charabati M,Sun T,Lee DSW,Galicia G,Ramaglia V,Ward LA,Leung LYT,Najafi G,Khaleghi K,Garcillán B,Li A,Besla R,Naouar I,Cao EY,Chiaranunt P,Burrows K,Robinson HG,Allanach JR,Yam J,Luck H,Campbell DJ,Allman D,Brooks DG,Tomura M,Baumann R,Zamvil SS,Bar-Or A,Horwitz MS,Winer DA,Mortha A,Mackay F,Prat A,Osborne LC,Robbins C,Baranzini SE,Gommerman JL

25-0081-82 was used in Flow Cytometry to show that IgA+ plasmablast and/or plasma cells mobilized from the gut play an role in suppressing neuroinflammation.

Thu Jan 24 00:00:00 EST 2019

Polychromic Reporter Mice Reveal Unappreciated Innate Lymphoid Cell Progenitor Heterogeneity and Elusive ILC3 Progenitors in Bone Marrow.

Immunity

Walker JA,Clark PA,Crisp A,Barlow JL,Szeto A,Ferreira ACF,Rana BMJ,Jolin HE,Rodriguez-Rodriguez N,Sivasubramaniam M,Pannell R,Cruickshank J,Daly M,Haim-Vilmovsky L,Teichmann SA,McKenzie ANJ

25-0081 was used in Flow cytometry/Cell sorting to identify a discrete ILC2-committed population and delineated transition states between early progenitors and a highly heterogeneous ILC1, ILC3, and NK precursor cell cluster.

Tue Jul 16 00:00:00 EDT 2019

GATA3-Controlled Nucleosome Eviction Drives MYC Enhancer Activity in T-cell Development and Leukemia.

Cancer discovery

Belver L,Yang AY,Albero R,Herranz D,Brundu FG,Quinn SA,Pérez-Durán P,Álvarez S,Gianni F,Rashkovan M,Gurung D,Rocha PP,Raviram R,Reglero C,Cortés JR,Cooke AJ,Wendorff AA,Cordó V,Meijerink JP,Rabadan R,Ferrando AA

25-0081 was used in Flow cytometry/Cell sorting to demonstrate a role for aberrant chromatin accessibility in the regulation of MYC expression in T-cell lymphoblastic leukemia (T-ALL).

Sun Dec 01 00:00:00 EST 2019

CD8⁺ T cells induce cachexia during chronic viral infection.

Nature immunology

Baazim H,Schweiger M,Moschinger M,Xu H,Scherer T,Popa A,Gallage S,Ali A,Khamina K,Kosack L,Vilagos B,Smyth M,Lercher A,Friske J,Merkler D,Aderem A,Helbich TH,Heikenwälder M,Lang PA,Zechner R,Bergthaler A

25-0081-82 was used in Flow Cytometry to identify the role for CD8+ T cells in infection-associated cachexia.

Sat Jun 01 00:00:00 EDT 2019

Engineering a haematopoietic stem cell niche by revitalizing mesenchymal stromal cells.

Nature cell biology

Nakahara F,Borger DK,Wei Q,Pinho S,Maryanovich M,Zahalka AH,Suzuki M,Cruz CD,Wang Z,Xu C,Boulais PE,Ma'ayan A,Greally JM,Frenette PS

25-0081-82 was used in Flow Cytometry to provide insight into the transcriptional regulation of the niche with implications for stem cell-based therapies.

Wed May 01 00:00:00 EDT 2019

Engulfment of mast cell secretory granules on skin inflammation boosts dendritic cell migration and priming efficiency.

The Journal of allergy and clinical immunology

Dudeck J,Froebel J,Kotrba J,Lehmann CHK,Dudziak D,Speier S,Nedospasov SA,Schraven B,Dudeck A

25-0081 was used in Flow cytometry/Cell sorting to show peripheral mast cells can modify dendritic cell functionality to influence lymphoid tissue-borne adaptive immunity.

Wed May 01 00:00:00 EDT 2019

miR-181a/b-1 controls thymic selection of Treg cells and tunes their suppressive capacity.

PLoS biology

Łyszkiewicz M,Winter SJ,Witzlau K,Föhse L,Brownlie R,Puchałka J,Verheyden NA,Kunze-Schumacher H,Imelmann E,Blume J,Raha S,Sekiya T,Yoshimura A,Frueh JT,Ullrich E,Huehn J,Weiss S,Gutierrez MG,Prinz I,Zamoyska R,Ziętara N,Krueger A

25-0081-82 was used in Flow Cytometry to propose that intrathymic miR-181a/b-1 controls development of Treg cells and imposes a developmental legacy on their peripheral function.

Fri Mar 01 00:00:00 EST 2019

Bilateral Effect of the Unilateral Corneal Nerve Cut on Both Ocular Surface and Lacrimal Gland.

Investigative ophthalmology & visual science

Lee HK,Kim KW,Ryu JS,Jeong HJ,Lee SM,Kim MK

25-0081 was used in Flow cytometry/Cell sorting to demonstrate that a unilateral cut of the corneal nerve results in activation of immune cells on the ocular surface and dysregulation of lacrimal secretion bilaterally through the bidirectional neuronal signals.

Wed Jan 02 00:00:00 EST 2019

Antiallodynic Effects of Cannabinoid Receptor 2 (CB₂R) Agonists on Retrovirus Infection-Induced Neuropathic Pain.

Pain research & management

Sheng WS,Chauhan P,Hu S,Prasad S,Lokensgard JR

25-0081-82 was used in Flow cytometry/Cell sorting to evaluate whether treatment with the exogenous synthetic CB2R agonists JWH015, JWH133, Gp1a, and HU308 controls neuropathic pain and neuroinflammation in animals with chronic retroviral infection.

Mon Dec 16 00:00:00 EST 2019

Antitumor and immunomodulatory effects of a novel multitarget inhibitor, CS2164, in mouse hepatocellular carcinoma models.

Anti-cancer drugs

Zhou Y,Fu C,Kong Y,Pan D,Wang Y,Huang S,Li Z,Ning Z,Lu X,Shan S,Xin L

25-0081 was used in Flow cytometry/Cell sorting to investigate the antitumor and immunomodulatory effects of CS2164.

Tue Oct 01 00:00:00 EDT 2019

TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases.

Cell reports

Yang BH,Wang K,Wan S,Liang Y,Yuan X,Dong Y,Cho S,Xu W,Jepsen K,Feng GS,Lu LF,Xue HH,Fu W

25-0081-82 was used in Flow Cytometry to study how CD4+ Foxp3+ T regulatory (Treg) cell differentiation and functional specification is regulated.

Tue Jun 18 00:00:00 EDT 2019

Neutralizing monoclonal antibody against Dickkopf2 impairs lung cancer progression via activating NK cells.

Cell death discovery

Shen T,Chen Z,Qiao J,Sun X,Xiao Q

25-0081-82 was used in Flow cytometry/Cell sorting to determine whether approaches targeting dysregulated adenomatous polyposis coli (APC) pathways are also applicable to non-small cell lung cancer patients harboring similar mutations of APC.

Tue Feb 13 00:00:00 EST 2024

Tpl2 Protects Against Fulminant Hepatitis Through Mobilization of Myeloid-Derived Suppressor Cells.

Frontiers in immunology

Xu J,Pei S,Wang Y,Liu J,Qian Y,Huang M,Zhang Y,Xiao Y

25-0081 was used in Flow cytometry/Cell sorting to establish Tpl2 as a critical mediator of MDSC recruitment and highlight the therapeutic potential of Tpl2 for the treatment of fulminant hepatitis (FH).

Wed Oct 14 00:00:00 EDT 2020

Interleukin-27 Is Essential for Type 1 Diabetes Development and Sjögren Syndrome-like Inflammation.

Cell reports

Ciecko AE,Foda B,Barr JY,Ramanathan S,Atkinson MA,Serreze DV,Geurts AM,Lieberman SM,Chen YG

25-0081-82 was used in Flow Cytometry to reveal that IL-27 contributes to autoimmunity in NOD mice through multiple mechanisms and provides substantial evidence to support its pathogenic role in human T1D.

Tue Dec 03 00:00:00 EST 2019

Expression of the Phosphatase Ppef2 Controls Survival and Function of CD8⁺ Dendritic Cells.

Frontiers in immunology

Zwick M,Ulas T,Cho YL,Ried C,Grosse L,Simon C,Bernhard C,Busch DH,Schultze JL,Buchholz VR,Stutte S,Brocker T

25-0081-82 was used in Flow Cytometry to suggest that Ppef2 is crucial to support survival of immature CD8+ DCs, while Ppef2 down-regulation during DC-maturation limits T cell responses.

Tue Oct 27 00:00:00 EDT 2020

Restricted Expression of the Thymoproteasome Is Required for Thymic Selection and Peripheral Homeostasis of CD8⁺ T Cells.

Cell reports

Tomaru U,Konno S,Miyajima S,Kimoto R,Onodera M,Kiuchi S,Murata S,Ishizu A,Kasahara M

25-0081 was used in Flow cytometry/Cell sorting to find that thymoproteasomes are essential for positive selection but that the subsequent change in peptide repertoire in the medulla is also crucial for thymic selection and that β5t-derived peptide must be confined to the thymus to avoid autoimmunity in peripheral tissues.

Tue Jan 15 00:00:00 EST 2019

DKK2 imparts tumor immunity evasion through β-catenin-independent suppression of cytotoxic immune-cell activation.

Nature medicine

Xiao Q,Wu J,Wang WJ,Chen S,Zheng Y,Yu X,Meeth K,Sahraei M,Bothwell ALM,Chen L,Bosenberg M,Chen J,Sexl V,Sun L,Li L,Tang W,Wu D

25-0081 was used in Flow cytometry/Cell sorting to identify a novel tumour immune-suppressive mechanism and immunotherapeutic targets particularly relevant for colorectal cancers and a subset of melanomas.

Thu Mar 01 00:00:00 EST 2018

Adrenergic nerve degeneration in bone marrow drives aging of the hematopoietic stem cell niche.

Nature medicine

Maryanovich M,Zahalka AH,Pierce H,Pinho S,Nakahara F,Asada N,Wei Q,Wang X,Ciero P,Xu J,Leftin A,Frenette PS

25-0081 was used in Flow cytometry/Cell sorting to determine whether haematopoietic stem cell ageing depends on bone marrow innervation by the sympathetic nervous system.

Fri Jun 01 00:00:00 EDT 2018

A human anti-IL-2 antibody that potentiates regulatory T cells by a structure-based mechanism.

Nature medicine

Trotta E,Bessette PH,Silveria SL,Ely LK,Jude KM,Le DT,Holst CR,Coyle A,Potempa M,Lanier LL,Garcia KC,Crellin NK,Rondon IJ,Bluestone JA

25-0081 was used in Flow cytometry/Cell sorting to suggest that IL-2-F5111.2 may provide an immunotherapy to treat autoimmune diseases and graft-versus-host disease.

Sun Jul 01 00:00:00 EDT 2018

CD226 regulates natural killer cell antitumor responses via phosphorylation-mediated inactivation of transcription factor FOXO1.

Proceedings of the National Academy of Sciences of the United States of America

Du X,de Almeida P,Manieri N,de Almeida Nagata D,Wu TD,Harden Bowles K,Arumugam V,Schartner J,Cubas R,Mittman S,Javinal V,Anderson KR,Warming S,Grogan JL,Chiang EY

25-0081 was used in Flow cytometry/Cell sorting to define a pathway by which CD226 exerts control of NK cell responses against tumors.

Tue Dec 11 00:00:00 EST 2018

Intranasal delivery of adjuvant-free peptide nanofibers elicits resident CD8⁺ T cell responses.

Journal of controlled release : official journal of the Controlled Release Society

Si Y,Wen Y,Kelly SH,Chong AS,Collier JH

25-0081 was used in Flow cytometry/Cell sorting to investigate the extent to which self-assembled peptide nanofibers bearing an influenza peptide epitope elicit antigen-specific CD8+ T cell responses when delivered intranasally, and compare these responses with those elicited by subcutaneous immunization.

Sat Jul 28 00:00:00 EDT 2018

The Severe Deficiency of the Somatotrope GH-Releasing Hormone/Growth Hormone/Insulin-Like Growth Factor 1 Axis of Mice Is Associated With an Important Splenic Atrophy and Relative B Lymphopenia.

Frontiers in endocrinology

Bodart G,Farhat K,Renard-Charlet C,Becker G,Plenevaux A,Salvatori R,Geenen V,Martens H

25-0081 was used in Flow cytometry/Cell sorting to examine the precise control exerted by the somatotrope growth hormone(GH)-releasing hormone/GH/insulin-like growth factor 1 axis on the immune system.

Sun Apr 10 00:00:00 EDT 2022

Bcl11b is essential for licensing Th2 differentiation during helminth infection and allergic asthma.

Nature communications

Lorentsen KJ,Cho JJ,Luo X,Zuniga AN,Urban JF,Zhou L,Gharaibeh R,Jobin C,Kladde MP,Avram D

25-0081 was used in Flow cytometry/Cell sorting to establish novel roles for Bcl11b in the regulatory loop that licenses Th2 program in vivo.

Thu Apr 26 00:00:00 EDT 2018

Spred1 Safeguards Hematopoietic Homeostasis against Diet-Induced Systemic Stress.

Cell stem cell

Tadokoro Y,Hoshii T,Yamazaki S,Eto K,Ema H,Kobayashi M,Ueno M,Ohta K,Arai Y,Hara E,Harada K,Oshima M,Oshima H,Arai F,Yoshimura A,Nakauchi H,Hirao A

25-0081-82 was used in Flow Cytometry to demonstrate the involvement of diet-induced stress on hematopoietic stem cell self-renewal.

Thu May 03 00:00:00 EDT 2018

Type 2 Innate Lymphocytes Actuate Immunity Against Tumours and Limit Cancer Metastasis.

Scientific reports

Saranchova I,Han J,Zaman R,Arora H,Huang H,Fenninger F,Choi KB,Munro L,Pfeifer CG,Welch I,Takei F,Jefferies WA

25-0081 was used in Flow cytometry/Cell sorting to study the role of type 2 innate lymphoid cells in enhancing anti-cancer immunity and controlling metastasis.

Tue Feb 13 00:00:00 EST 2018

Cyclophilin J limits inflammation through the blockage of ubiquitin chain sensing.

Nature communications

Sheng C,Yao C,Wang Z,Chen H,Zhao Y,Xu D,Huang H,Huang W,Chen S

25-0081-82 was used in flow cytometry following flow cytometry to identify cyclophilin J as a negative feedback regulator of the NF-κB signalling pathway.

Mon Oct 22 00:00:00 EDT 2018

Photosensitizer Micelles Together with IDO Inhibitor Enhance Cancer Photothermal Therapy and Immunotherapy.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Peng J,Xiao Y,Li W,Yang Q,Tan L,Jia Y,Qu Y,Qian Z

25-0081 was used in Flow cytometry/Cell sorting to demonstrate that NLG919/IR780 micelles combine PTT, immunotherapy, suppress the tumour margin and distal tumour growth post photothermal therapy.

Tue May 01 00:00:00 EDT 2018

Efficient immunoaffinity chromatography of lymphocytes directly from whole blood.

Scientific reports

Mohr F,Przibilla S,Leonhardt F,Stemberger C,Dreher S,Müller TR,Fräßle SP,Schmidt GP,Kiene ML,Stadler H,Busch DH

25-0081 was used in Flow cytometry/Cell sorting to show that defined lymphocytes can be rapidly purified by immunoaffinity chromatography starting directly from whole blood.

Tue Nov 13 00:00:00 EST 2018

A Plasmodium Cross-Stage Antigen Contributes to the Development of Experimental Cerebral Malaria.

Frontiers in immunology

Fernandes P,Howland SW,Heiss K,Hoffmann A,Hernández-Castañeda MA,Obrová K,Frank R,Wiedemann P,Bendzus M,Rénia L,Mueller AK

25-0081 was used in Flow cytometry/Cell sorting to highlight the importance of the natural route of infection in the study of experimental cerebral malaria with potentials implications for vaccine and therapeutic strategies against malaria.

Wed Sep 18 00:00:00 EDT 2019

Limited Foxp3⁺ Regulatory T Cells Response During Acute Trypanosoma cruzi Infection Is Required to Allow the Emergence of Robust Parasite-Specific CD8⁺ T Cell Immunity.

Frontiers in immunology

Araujo Furlan CL,Tosello Boari J,Rodriguez C,Canale FP,Fiocca Vernengo F,Boccardo S,Beccaria CG,Adoue V,Joffre O,Gruppi A,Montes CL,Acosta Rodriguez EV

25-0081 was used in Flow cytometry/Cell sorting to study the role of regulatory T cells during Trypanosoma cruzi infection.

Thu Sep 26 00:00:00 EDT 2019

Vitamin A-Retinoic Acid Signaling Regulates Hematopoietic Stem Cell Dormancy.

Cell

Cabezas-Wallscheid N,Buettner F,Sommerkamp P,Klimmeck D,Ladel L,Thalheimer FB,Pastor-Flores D,Roma LP,Renders S,Zeisberger P,Przybylla A,Schönberger K,Scognamiglio R,Altamura S,Florian CM,Fawaz M,Vonficht D,Tesio M,Collier P,Pavlinic D,Geiger H,Schroeder T,Benes V,Dick TP,Rieger MA,Stegle O,Trumpp A

25-0081 was used in Flow cytometry/Cell sorting to highlight the impact of dietary vitamin A on the regulation of cell-cycle-mediated stem cell plasticity.

Thu May 18 00:00:00 EDT 2017

Identification of Nascent Memory CD8 T Cells and Modeling of Their Ontogeny.

Cell systems

Crauste F,Mafille J,Boucinha L,Djebali S,Gandrillon O,Marvel J,Arpin C

25-0081-82 was used in Flow Cytometry to identify Bcl2 and Mki67 as a marker combination that enables the tracking of nascent memory cells within the effector phase.

Wed Mar 22 00:00:00 EDT 2017

Prenatal Administration of Betamethasone Causes Changes in the T Cell Receptor Repertoire Influencing Development of Autoimmunity.

Frontiers in immunology

Gieras A,Gehbauer C,Perna-Barrull D,Engler JB,Diepenbruck I,Glau L,Joosse SA,Kersten N,Klinge S,Mittrücker HW,Friese MA,Vives-Pi M,Tolosa E

25-0081 was used in Flow cytometry/Cell sorting to demonstrate that prenatal administration of betamethasone causes changes in the T cell receptor repertoire influencing development of autoimmunity.

Wed Nov 20 00:00:00 EST 2019

MYC selects against reduced BCL2A1/A1 protein expression during B cell lymphomagenesis.

Oncogene

Sochalska M,Schuler F,Weiss JG,Prchal-Murphy M,Sexl V,Villunger A

25-0081 was used in Flow cytometry/Cell sorting to analyse the role of the anti-apoptotic protein, BCL2A1/A1, in blood cancers driven by either the MYC or ABL kinases.

Sat Apr 01 00:00:00 EDT 2017

AIM2 Inflammasome Is Critical for Influenza-Induced Lung Injury and Mortality.

Journal of immunology (Baltimore, Md. : 1950)

Zhang H,Luo J,Alcorn JF,Chen K,Fan S,Pilewski J,Liu A,Chen W,Kolls JK,Wang J

25-0081 was used in Flow cytometry/Cell sorting to demonstrate that influenza infection activates the AIM2 inflammasome, which plays a critical role in IAV-induced lung injury and mortality.

Thu Jun 01 00:00:00 EDT 2017

Fractional Laser Releases Tumor-Associated Antigens in Poorly Immunogenic Tumor and Induces Systemic Immunity.

Scientific reports

Kawakubo M,Cunningham TJ,Demehri S,Manstein D

25-0081 was used in Flow cytometry/Cell sorting to assess the utility of ablative fractional photothermolysis for treating oncological indications.

Fri Oct 06 00:00:00 EDT 2017

A Burkholderia pseudomallei Outer Membrane Vesicle Vaccine Provides Cross Protection against Inhalational Glanders in Mice and Non-Human Primates.

Vaccines

Baker SM,Davitt CJH,Motyka N,Kikendall NL,Russell-Lodrigue K,Roy CJ,Morici LA

25-0081 was used in Flow cytometry/Cell sorting to demonstrate the role of Burkholderia pseudomallei outer membrane vesicle vaccines in cross protection against inhalationalglanders.

Sat Dec 09 00:00:00 EST 2017

Histone/protein deacetylase 11 targeting promotes Foxp3+ Treg function.

Scientific reports

Huang J,Wang L,Dahiya S,Beier UH,Han R,Samanta A,Bergman J,Sotomayor EM,Seto E,Kozikowski AP,Hancock WW

25-0081 was used in Flow cytometry/Cell sorting to examine the effects of global histone/protein deacetylase inhibition on regulatory T cell function.

Thu Aug 17 00:00:00 EDT 2017

Paeoniflorin Ameliorates Experimental Autoimmune Encephalomyelitis via Inhibition of Dendritic Cell Function and Th17 Cell Differentiation.

Scientific reports

Zhang H,Qi Y,Yuan Y,Cai L,Xu H,Zhang L,Su B,Nie H

25-0081 was used in Flow cytometry/Cell sorting to investigate the effect Paeoniflorin has on Th cells and dendritic cells in a mouse MS model.

Mon Feb 06 00:00:00 EST 2017

Tregitopes and impaired antigen presentation: Drivers of the immunomodulatory effects of IVIg?

Immunity, inflammation and disease

Sordé L,Spindeldreher S,Palmer E,Karle A

25-0081 was used in Flow cytometry/Cell sorting to study the potential impact of IVIg on the ability of antigen presenting cells to present peptides.

Fri Dec 01 00:00:00 EST 2017

Fractional laser exposure induces neutrophil infiltration (N1 phenotype) into the tumor and stimulates systemic anti-tumor immune response.

PloS one

Kawakubo M,Demehri S,Manstein D

25-0081 was used in Flow cytometry/Cell sorting to explore the utility of ablative fractional photothermolysis in treating oncological indications, including induction of local tumour regression and inducing anti-tumour immunity.

Fri Oct 13 00:00:00 EDT 2017

Cooperation of Oncolytic Herpes Virotherapy and PD-1 Blockade in Murine Rhabdomyosarcoma Models.

Scientific reports

Chen CY,Wang PY,Hutzen B,Sprague L,Swain HM,Love JK,Stanek JR,Boon L,Conner J,Cripe TP

25-0081 was used in Flow cytometry/Cell sorting to show the combination of PD-1 blockade and oHSV-1 may be an effective treatment strategy for childhood soft tissue sarcoma.

Wed May 24 00:00:00 EDT 2017

Lymphatic vessels regulate immune microenvironments in human and murine melanoma.

The Journal of clinical investigation

Lund AW,Wagner M,Fankhauser M,Steinskog ES,Broggi MA,Spranger S,Gajewski TF,Alitalo K,Eikesdal HP,Wiig H,Swartz MA

25-0081 was used in Flow cytometry/Cell sorting to suggest that the establishment of tumour-associated inflammation and immunity critically depends on lymphatic vessel remodelling and drainage.

Thu Sep 01 00:00:00 EDT 2016

mTOR has distinct functions in generating versus sustaining humoral immunity.

The Journal of clinical investigation

Jones DD,Gaudette BT,Wilmore JR,Chernova I,Bortnick A,Weiss BM,Allman D

25-0081 was used in Flow cytometry/Cell sorting to conclude that mTOR signalling plays critical but diverse roles in early and late phases of antibody responses and plasma cell differentiation.

Tue Nov 01 00:00:00 EDT 2016

Resolving TYK2 locus genotype-to-phenotype differences in autoimmunity.

Science translational medicine

Dendrou CA,Cortes A,Shipman L,Evans HG,Attfield KE,Jostins L,Barber T,Kaur G,Kuttikkatte SB,Leach OA,Desel C,Faergeman SL,Cheeseman J,Neville MJ,Sawcer S,Compston A,Johnson AR,Everett C,Bell JI,Karpe F,Ultsch M,Eigenbrot C,McVean G,Fugger L

25-0081 was used in Flow cytometry/Cell sorting to reveal a protective homozygous effect that defined a signalling optimum between autoimmunity and immunodeficiency and identified TYK2 as a potential drug target for autoimmune disorders.

Wed Nov 02 00:00:00 EDT 2016

MicroRNA-7 Deficiency Ameliorates the Pathologies of Acute Lung Injury through Elevating KLF4.

Frontiers in immunology

Zhao J,Chen C,Guo M,Tao Y,Cui P,Zhou Y,Qin N,Zheng J,Zhang J,Xu L

25-0081 was used in Flow cytometry/Cell sorting to study the role of microRNA-7 in acute lung injury, allowing the development of new therapeutic strategies against inflammatory lung disease.

Wed Mar 16 00:00:00 EDT 2022

IL-33-Dependent Group 2 Innate Lymphoid Cells Promote Cutaneous Wound Healing.

The Journal of investigative dermatology

Rak GD,Osborne LC,Siracusa MC,Kim BS,Wang K,Bayat A,Artis D,Volk SW

25-0081 was used in Flow cytometry/Cell sorting to demonstrate that cutaneous injury promotes an IL-33-dependent group 2 ILC response and that abrogation of this response can impair efficient wound closure.

Mon Feb 01 00:00:00 EST 2016

Lineage tracing of cells involved in atherosclerosis.

Atherosclerosis

Albarrán-Juárez J,Kaur H,Grimm M,Offermanns S,Wettschureck N

25-0081 was used in Flow cytometry/Cell sorting to identify the origin of vascular smooth muscle cells and macrophages within atherosclerosis lesions.

Mon Aug 01 00:00:00 EDT 2016

G-CSF-Induced Suppressor IL-10+ Neutrophils Promote Regulatory T Cells That Inhibit Graft-Versus-Host Disease in a Long-Lasting and Specific Way.

Journal of immunology (Baltimore, Md. : 1950)

Perobelli SM,Mercadante AC,Galvani RG,Gonçalves-Silva T,Alves AP,Pereira-Neves A,Benchimol M,Nóbrega A,Bonomo A

25-0081 was used in Flow cytometry/Cell sorting to study the role of incoming IL-10+ neutrophils from G-CSF-treated donor spleen in inhibiting acute graft-versus-host disease.

Tue Nov 01 00:00:00 EDT 2016

Contamination of DNase Preparations Confounds Analysis of the Role of DNA in Alum-Adjuvanted Vaccines.

Journal of immunology (Baltimore, Md. : 1950)

Noges LE,White J,Cambier JC,Kappler JW,Marrack P

25-0081 was used in Flow cytometry/Cell sorting to investigate the contribution of host DNA released at the site of vaccine injection to the adjuvant activity of alum.

Mon Aug 15 00:00:00 EDT 2016

Azacytidine mitigates experimental sclerodermic chronic graft-versus-host disease.

Journal of hematology & oncology

Fransolet G,Ehx G,Somja J,Delens L,Hannon M,Muller J,Dubois S,Drion P,Caers J,Humblet-Baron S,Delvenne P,Beguin Y,Conteduca G,Baron F

25-0081 was used in Flow cytometry/Cell sorting to assessed the impact of azacytidine on cGVHD in a murine model of sclerodermic cGVHD.

Mon Jul 04 00:00:00 EDT 2016

Host protective ASP-based vaccine against the parasitic nematode Ostertagia ostertagi triggers NK cell activation and mixed IgG1-IgG2 response.

Scientific reports

González-Hernández A,Van Coppernolle S,Borloo J,Van Meulder F,Paerewijck O,Peelaers I,Leclercq G,Claerebout E,Geldhof P

25-0081 was used in Flow cytometry/Cell sorting to elucidate the immunological importance of both the antigen and adjuvant: nASP and QuilA respectively, in a Ostertagia ostertagi vaccine.

Mon Jul 11 00:00:00 EDT 2016

Tetanus toxoid and CCL3 improve dendritic cell vaccines in mice and glioblastoma patients.

Nature

Mitchell DA,Batich KA,Gunn MD,Huang MN,Sanchez-Perez L,Nair SK,Congdon KL,Reap EA,Archer GE,Desjardins A,Friedman AH,Friedman HS,Herndon JE,Coan A,McLendon RE,Reardon DA,Vredenburgh JJ,Bigner DD,Sampson JH

25-0081 was used in Flow cytometry/Cell sorting to show that pre-conditioning with a potent recall antigen significantly improves the homing ability and efficacy of tumour-antigen-specific dendritic cells.

Thu Mar 19 00:00:00 EDT 2015

CXCR4 expression on pathogenic T cells facilitates their bone marrow infiltration in a mouse model of aplastic anemia.

Blood

Arieta Kuksin C,Gonzalez-Perez G,Minter LM

25-0081 was used in Flow cytometry/Cell sorting to demonstrate that CXCR4 mediates migration of pathogenic T-cells to the bone marrow in aplastic anemia mice.

Thu Mar 26 00:00:00 EDT 2015

Hematopoietic stem cell quiescence and function are controlled by the CYLD-TRAF2-p38MAPK pathway.

The Journal of experimental medicine

Tesio M,Tang Y,Müdder K,Saini M,von Paleske L,Macintyre E,Pasparakis M,Waisman A,Trumpp A

25-0081 was used in Flow cytometry/Cell sorting to elucidate the molecular mechanisms regulating haematopoietic stem cell dormancy.

Mon Apr 06 00:00:00 EDT 2015

A Threshold Level of Intratumor CD8+ T-cell PD1 Expression Dictates Therapeutic Response to Anti-PD1.

Cancer research

Ngiow SF,Young A,Jacquelot N,Yamazaki T,Enot D,Zitvogel L,Smyth MJ

25-0081 was used in Flow cytometry/Cell sorting to provide a framework to interrogate intra-tumour CD8(+) T-cell PD1 and immune PDL1 levels and response in human cancer.

Tue Sep 15 00:00:00 EDT 2015

Mast cells boost myeloid-derived suppressor cell activity and contribute to the development of tumor-favoring microenvironment.

Cancer immunology research

Danelli L,Frossi B,Gri G,Mion F,Guarnotta C,Bongiovanni L,Tripodo C,Mariuzzi L,Marzinotto S,Rigoni A,Blank U,Colombo MP,Pucillo CE

25-0081 was used in Flow cytometry/Cell sorting to examine the interactions between myeloid-derived suppressor cells and mast cells in the mucosa of patients with colon carcinoma.

Thu Jan 01 00:00:00 EST 2015

Premature thymic involution is independent of structural plasticity of the thymic stroma.

European journal of immunology

Franckaert D,Schlenner SM,Heirman N,Gill J,Skogberg G,Ekwall O,Put K,Linterman MA,Dooley J,Liston A

25-0081 was used in Flow cytometry/Cell sorting to investigate thymic involution in FVB/N mice, showing that disruption of the epithelial-endothelial relationship and a progressive loss of pro-T cells precedes involution, and loss of pro-T cells is sufficient to drive premature involution, suggesting that pro-T cells are the main driver of involution.

Fri May 01 00:00:00 EDT 2015

Hind limb unloading, a model of spaceflight conditions, leads to decreased B lymphopoiesis similar to aging.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Lescale C,Schenten V,Djeghloul D,Bennabi M,Gaignier F,Vandamme K,Strazielle C,Kuzniak I,Petite H,Dosquet C,Frippiat JP,Goodhardt M

25-0081 was used in Flow cytometry/Cell sorting to demonstrate that mechanical unloading of hind limbs results in a decrease in early B-cell differentiation resembling age-related modifications in B lymphopoiesis.

Sun Feb 01 00:00:00 EST 2015

IFN-β Selectively Inhibits IL-2 Production through CREM-Mediated Chromatin Remodeling.

Journal of immunology (Baltimore, Md. : 1950)

Otero DC,Fares-Frederickson NJ,Xiao M,Baker DP,David M

25-0081 was used in Flow cytometry/Cell sorting to investigate the mechanism by which interferon beta limits T cell responses and its potential therapeutic use for multiple sclerosis.

Mon Jun 01 00:00:00 EDT 2015

T-bet Is Required for the Rapid Clearance of Attenuated Rabies Virus from Central Nervous System Tissue.

Journal of immunology (Baltimore, Md. : 1950)

Lebrun A,Portocarrero C,Kean RB,Barkhouse DA,Faber M,Hooper DC

25-0081 was used in Flow cytometry/Cell sorting to study the role of CD4 T cell subsets in the clearance of attenuated rabies virus from central nervous system tissues.

Sun Nov 01 00:00:00 EDT 2015

Excessive proliferation and impaired function of primitive hematopoietic cells in bone marrow due to senescence post chemotherapy in a T cell acute lymphoblastic leukemia model.

Journal of translational medicine

Jiang C,Hu X,Wang L,Cheng H,Lin Y,Pang Y,Yuan W,Cheng T,Wang J

25-0081 was used in Flow cytometry/Cell sorting to conclude that primitive hematopoietic cells in bone marrow enter proliferation earlier than leukemic cells after chemotherapy, and gradually lose their regenerative capacity partly by senescence due to accelerated cycling.

Fri Jul 17 00:00:00 EDT 2015

Bi-Allelic TCRα or β Recombination Enhances T Cell Development but Is Dispensable for Antigen Responses and Experimental Autoimmune Encephalomyelitis.

PloS one

Schuldt NJ,Auger JL,Hogquist KA,Binstadt BA

25-0081 was used in Flow cytometry/Cell sorting to investigate dual TCRβ expression in T cells.

Thu Jun 23 00:00:00 EDT 2016

Toxoplasma gondii superinfection and virulence during secondary infection correlate with the exact ROP5/ROP18 allelic combination.

mBio

Jensen KD,Camejo A,Melo MB,Cordeiro C,Julien L,Grotenbreg GM,Frickel EM,Ploegh HL,Young L,Saeij JP

25-0081 was used in Flow cytometry/Cell sorting to investigate the interaction between infected host and Toxoplasma gondii strains in endemic regions, showing that secondary infection correlates with the ROP5/ROP18 allele combinations.

Tue Feb 24 00:00:00 EST 2015

Validation of a multicolor staining to monitor phosphoSTAT5 levels in regulatory T-cell subsets.

Oncotarget

Ehx G,Hannon M,Beguin Y,Humblet-Baron S,Baron F

25-0081 was used in Flow cytometry/Cell sorting to evaluate a novel staining method with the potential to monitor the responses of various Treg subsets to IL-2 therapy.

Tue Dec 22 00:00:00 EST 2015

Inhibition of iNOS activity enhances the anti-tumor effects of alpha-galactosylceramide in established murine cancer model.

Oncotarget

Ito H,Ando T,Seishima M

25-0081 was used in Flow cytometry/Cell sorting to investigate how the inhibition of iNOS expression could enhance the therapeutic efficacy of GalCer via the increase of tumor antigen-specific immune response and the suppression of MDSCs.

Tue Dec 08 00:00:00 EST 2015

Local Salmonella immunostimulation recruits vaccine-specific CD8 T cells and increases regression of bladder tumor.

Oncoimmunology

Domingos-Pereira S,Hojeij R,Reggi E,Derré L,Chevalier MF,Romero P,Jichlinski P,Nardelli-Haefliger D

25-0081 was used in Flow cytometry/Cell sorting to demonstrate that combining vaccination with local immunostimulation may be an effective treatment strategy for different types of cancer.

Wed Jul 01 00:00:00 EDT 2015

Expression of a Chimeric Antigen Receptor in Multiple Leukocyte Lineages in Transgenic Mice.

PloS one

Yong CS,Westwood JA,Schröder J,Papenfuss AT,von Scheidt B,Moeller M,Devaud C,Darcy PK,Kershaw MH

25-0081 was used in Flow cytometry/Cell sorting to generate a mouse model expressing chimeric antigen receptors (CARs) specific for a tumour antigen under a pan-hematopoietic promoter.

Wed Jun 08 00:00:00 EDT 2016

Sunitinib depletes myeloid-derived suppressor cells and synergizes with a cancer vaccine to enhance antigen-specific immune responses and tumor eradication.

Oncoimmunology

Draghiciu O,Nijman HW,Hoogeboom BN,Meijerhof T,Daemen T

25-0081 was used in Flow cytometry/Cell sorting to demonstrate that sunitinib can create a favourable microenvironment depleted of MDSCs, and act synergistically with a cancer vaccine, resulting in enhanced levels of active tumor-antigen specific CTLs.

Sun Mar 01 00:00:00 EST 2015

A NOTCH1-driven MYC enhancer promotes T cell development, transformation and acute lymphoblastic leukemia.

Nature medicine

Herranz D,Ambesi-Impiombato A,Palomero T,Schnell SA,Belver L,Wendorff AA,Xu L,Castillo-Martin M,Llobet-Navás D,Cordon-Cardo C,Clappier E,Soulier J,Ferrando AA

25-0081 was used in Flow cytometry/Cell sorting to identify N-Me as a long-range oncogenic enhancer implicated directly in the pathogenesis of human leukaemia.

Wed Oct 01 00:00:00 EDT 2014

Megakaryocytes regulate hematopoietic stem cell quiescence through CXCL4 secretion.

Nature medicine

Bruns I,Lucas D,Pinho S,Ahmed J,Lambert MP,Kunisaki Y,Scheiermann C,Schiff L,Poncz M,Bergman A,Frenette PS

25-0081 was used in Flow cytometry/Cell sorting to indicate that a terminally differentiated cell type derived from HSCs contributes to the HSC niche, directly regulating HSC behavior.

Sat Nov 01 00:00:00 EDT 2014

Mycobacteria manipulate macrophage recruitment through coordinated use of membrane lipids.

Nature

Cambier CJ,Takaki KK,Larson RP,Hernandez RE,Tobin DM,Urdahl KB,Cosma CL,Ramakrishnan L

25-0081 was used in Flow cytometry/Cell sorting to show, in mice and zebrafish, that M. tuberculosis preferentially recruits and infects permissive macrophages while evading microbicidal ones.

Thu Jan 09 00:00:00 EST 2014

Foxp3+ CD4+ T cells improve healing after myocardial infarction by modulating monocyte/macrophage differentiation.

Circulation research

Weirather J,Hofmann UD,Beyersdorf N,Ramos GC,Vogel B,Frey A,Ertl G,Kerkau T,Frantz S

25-0081 was used in Flow cytometry/Cell sorting to determine the mechanisms by which regulatory T cells promote an appropriate healing response after a heart attack.

Fri Jun 20 00:00:00 EDT 2014

The Alarmin HMGN1 contributes to antitumor immunity and is a potent immunoadjuvant.

Cancer research

Wei F,Yang D,Tewary P,Li Y,Li S,Chen X,Howard OM,Bustin M,Oppenheim JJ

25-0081 was used in Flow cytometry/Cell sorting to demonstrate the alarmin HMGN1 contributes to antitumor immunity and may offer an effective adjuvant to heighten responses to cancer vaccines.

Sat Nov 01 00:00:00 EDT 2014

Alphavirus-based vaccines encoding nonstructural proteins of hepatitis C virus induce robust and protective T-cell responses.

Molecular therapy : the journal of the American Society of Gene Therapy

Ip PP,Boerma A,Regts J,Meijerhof T,Wilschut J,Nijman HW,Daemen T

25-0081 was used in Flow cytometry/Cell sorting to study an rSFV-based vector which induces robust T-cell activity and has a potential for the treatment of HCV infections.

Tue Apr 01 00:00:00 EDT 2014

Tissues in different anatomical sites can sculpt and vary the tumor microenvironment to affect responses to therapy.

Molecular therapy : the journal of the American Society of Gene Therapy

Devaud C,Westwood JA,John LB,Flynn JK,Paquet-Fifield S,Duong CP,Yong CS,Pegram HJ,Stacker SA,Achen MG,Stewart TJ,Snyder LA,Teng MW,Smyth MJ,Darcy PK,Kershaw MH

25-0081 was used in Flow cytometry/Cell sorting to explore the relationship between tumour microenvironments and efficacy of immunotherapy.

Wed Jan 01 00:00:00 EST 2014

Lymph node stromal cells constrain immunity via MHC class II self-antigen presentation.

eLife

Baptista AP,Roozendaal R,Reijmers RM,Koning JJ,Unger WW,Greuter M,Keuning ED,Molenaar R,Goverse G,Sneeboer MM,den Haan JM,Boes M,Mebius RE

25-0081 was used in Flow cytometry/Cell sorting to investigate the role of MHC-II in lymph node stromal cells, showing that stromal cells constrain immunity through MHC-II expression.

Wed Nov 19 00:00:00 EST 2014

Intranasal vaccination promotes detrimental Th17-mediated immunity against influenza infection.

PLoS pathogens

Maroof A,Yorgensen YM,Li Y,Evans JT

25-0081 was used in Flow cytometry/Cell sorting to highlight the importance of both route of vaccination and adjuvant selection in vaccine development.

Wed Jan 01 00:00:00 EST 2014

Bacterial superantigens promote acute nasopharyngeal infection by Streptococcus pyogenes in a human MHC Class II-dependent manner.

PLoS pathogens

Kasper KJ,Zeppa JJ,Wakabayashi AT,Xu SX,Mazzuca DM,Welch I,Baroja ML,Kotb M,Cairns E,Cleary PP,Haeryfar SM,McCormick JK

25-0081 was used in Flow cytometry/Cell sorting to study how secreted superantigens contribute to the fitness and evolution of S. pyogenes.

Thu May 01 00:00:00 EDT 2014

Ectopic TBX1 suppresses thymic epithelial cell differentiation and proliferation during thymus organogenesis.

Development (Cambridge, England)

Reeh KA,Cardenas KT,Bain VE,Liu Z,Laurent M,Manley NR,Richie ER

25-0081 was used in Flow cytometry/Cell sorting to test the hypothesis that Tbx1 negatively regulates thymic epithelial cell growth and differentiation.

Fri Aug 01 00:00:00 EDT 2014

Virus-specific memory CD8 T cells provide substantial protection from lethal severe acute respiratory syndrome coronavirus infection.

Journal of virology

Channappanavar R,Fett C,Zhao J,Meyerholz DK,Perlman S

25-0081 was used in Flow cytometry/Cell sorting to determine the factors governing a successful immune response to lethal SARS-CoV challenge.

Wed Oct 01 00:00:00 EDT 2014

Eosinophils regulate peripheral B cell numbers in both mice and humans.

Journal of immunology (Baltimore, Md. : 1950)

Wong TW,Doyle AD,Lee JJ,Jelinek DF

25-0081 was used in Flow cytometry/Cell sorting to assess the effect of eosinophils on B cell survival, proliferation, and Ig secretion.

Tue Apr 15 00:00:00 EDT 2014

Oral combined therapy with probiotics and alloantigen induces B cell-dependent long-lasting specific tolerance.

Journal of immunology (Baltimore, Md. : 1950)

Mercadante AC,Perobelli SM,Alves AP,Gonçalves-Silva T,Mello W,Gomes-Santos AC,Miyoshi A,Azevedo V,Faria AM,Bonomo A

25-0081 was used in Flow cytometry/Cell sorting to suggest that combined therapy is a promising strategy for prevention of GVHD with preservation of graft-versus-leukemia in allogenic HSCT.

Sat Feb 15 00:00:00 EST 2014

Natural history of tumor growth and immune modulation in common spontaneous murine mammary tumor models.

Breast cancer research and treatment

Gad E,Rastetter L,Slota M,Koehnlein M,Treuting PM,Dang Y,Stanton S,Disis ML

25-0081 was used in Flow cytometry/Cell sorting to evaluate the relationship between growth rates of tumours in common spontaneous mammary tumor models and immune biomarkers evaluated in the tumour and blood.

Mon Dec 01 00:00:00 EST 2014

Conditional deletion of ferritin h in mice reduces B and T lymphocyte populations.

PloS one

Vanoaica L,Richman L,Jaworski M,Darshan D,Luther SA,Kühn LC

25-0081 was used in Flow cytometry/Cell sorting to investigate the effect of ferritin H deletion on lymphocytes, showing that it reduces B and T lymphocyte populations.

Tue Jan 13 00:00:00 EST 2015

Establishment of a murine graft-versus-myeloma model using allogeneic stem cell transplantation.

PloS one

Binsfeld M,Beguin Y,Belle L,Otjacques E,Hannon M,Briquet A,Heusschen R,Drion P,Zilberberg J,Bogen B,Baron F,Caers J

25-0081 was used in Flow cytometry/Cell sorting to investigate the anti-myeloma effects of allo-SCT from B10.D2 mice into MHC-matched myeloma-bearing Balb/cJ mice.

Tue Dec 15 00:00:00 EST 2015

Ly49E expression on CD8αα-expressing intestinal intraepithelial lymphocytes plays no detectable role in the development and progression of experimentally induced inflammatory bowel diseases.

PloS one

Van Acker A,Filtjens J,Van Welden S,Taveirne S,Van Ammel E,Vanhees M,Devisscher L,Kerre T,Taghon T,Vandekerckhove B,Plum J,Leclercq G

25-0081 was used in Flow cytometry/Cell sorting to characterise the role of Ly49E receptor expression on CD8αα-expressing innate-like intestinal intraepithelial lymphocytes in the development of inflammatory bowel disease.

Thu Sep 17 00:00:00 EDT 2015

Autophagy is a critical regulator of memory CD8(+) T cell formation.

eLife

Puleston DJ,Zhang H,Powell TJ,Lipina E,Sims S,Panse I,Watson AS,Cerundolo V,Townsend AR,Klenerman P,Simon AK

25-0081 was used in Flow cytometry/Cell sorting to show that mice lacking the autophagy gene Atg7 in T cells failed to establish CD8(+) T cell memory to influenza and MCMV infection.

Tue Nov 11 00:00:00 EST 2014

Three weeks of murine hindlimb unloading induces shifts from B to T and from th to tc splenic lymphocytes in absence of stress and differentially reduces cell-specific mitogenic responses.

PloS one

Gaignier F,Schenten V,De Carvalho Bittencourt M,Gauquelin-Koch G,Frippiat JP,Legrand-Frossi C

25-0081 was used in Functional assays to suggest that B cells are more affected by hindlimb unloading than T cells, which may explain the high susceptibility to pathogens.

Thu Jan 15 00:00:00 EST 2015

Discriminating cellular heterogeneity using microwell-based RNA cytometry.

Nature communications

Dimov IK,Lu R,Lee EP,Seita J,Sahoo D,Park SM,Weissman IL,Lee LP

25-0081 was used in Flow cytometry/Cell sorting to develop a two-stage system to determine cellular heterogeneity.

Tue Mar 25 00:00:00 EDT 2014

mRNA-based vaccines synergize with radiation therapy to eradicate established tumors.

Radiation oncology (London, England)

Fotin-Mleczek M,Zanzinger K,Heidenreich R,Lorenz C,Kowalczyk A,Kallen KJ,Huber SM

25-0081 was used in Flow cytometry/Cell sorting to investigate how mRNA-based vaccines interact with radiation therapy in treating established tumours.

Fri Aug 15 00:00:00 EDT 2014

The nucleotide sugar UDP-glucose mobilizes long-term repopulating primitive hematopoietic cells.

The Journal of clinical investigation

Kook S,Cho J,Lee SB,Lee BC

25-0081 was used in Flow cytometry/Cell sorting to demonstrate a novel mechanism governing the migration of haematopoietic stem progenitor cells under stress.

Thu Aug 01 00:00:00 EDT 2013

Host DNA released in response to aluminum adjuvant enhances MHC class II-mediated antigen presentation and prolongs CD4 T-cell interactions with dendritic cells.

Proceedings of the National Academy of Sciences of the United States of America

McKee AS,Burchill MA,Munks MW,Jin L,Kappler JW,Friedman RS,Jacobelli J,Marrack P

25-0081 was used in Flow cytometry/Cell sorting to study how aluminium acts as an adjuvant by introducing host DNA into the cytoplasm of dendritic cells, where it engages receptors promoting MHC II presentation.

Tue Mar 19 00:00:00 EDT 2013

Mesenchymal stem cells are mobilized from the bone marrow during inflammation.

Frontiers in immunology

Koning JJ,Kooij G,de Vries HE,Nolte MA,Mebius RE

25-0081 was used in Flow cytometry/Cell sorting to analyse mesenchymal stem- and T-cell migration during experimental allergic encephalomyelitis.

Wed Mar 06 00:00:00 EST 2013

Intravaginal TLR agonists increase local vaccine-specific CD8 T cells and human papillomavirus-associated genital-tumor regression in mice.

Mucosal immunology

Domingos-Pereira S,Decrausaz L,Derré L,Bobst M,Romero P,Schiller JT,Jichlinski P,Nardelli-Haefliger D

25-0081 was used in Flow cytometry/Cell sorting to study the immune effects of the instillation of Toll-like receptor agonists after subcutaneous E7 vaccination.

Fri Mar 01 00:00:00 EST 2013

Homologous recombination into the eosinophil peroxidase locus generates a strain of mice expressing Cre recombinase exclusively in eosinophils.

Journal of leukocyte biology

Doyle AD,Jacobsen EA,Ochkur SI,Willetts L,Shim K,Neely J,Kloeber J,Lesuer WE,Pero RS,Lacy P,Moqbel R,Lee NA,Lee JJ

25-0081 was used in Flow cytometry/Cell sorting to develop a novel murine strain to allow a better definition of the role of eosinophils in health and disease.

Mon Jul 01 00:00:00 EDT 2013

Coactivator-associated arginine methyltransferase 1 regulates fetal hematopoiesis and thymocyte development.

Journal of immunology (Baltimore, Md. : 1950)

Li J,Zhao Z,Carter C,Ehrlich LI,Bedford MT,Richie ER

25-0081 was used in Flow cytometry/Cell sorting to demonstrate how CARM1 is a key epigenetic regulator of hematopoiesis that affects multiple lineages at various stages of differentiation.

Tue Jan 15 00:00:00 EST 2013

Paracaspase MALT1 deficiency protects mice from autoimmune-mediated demyelination.

Journal of immunology (Baltimore, Md. : 1950)

Mc Guire C,Wieghofer P,Elton L,Muylaert D,Prinz M,Beyaert R,van Loo G

25-0081 was used in Flow cytometry/Cell sorting to study the role of MALT1 in the development of experimental autoimmune encephalomyelitis.

Fri Mar 15 00:00:00 EDT 2013

Phenotypic CD8+ T cell diversification occurs before, during, and after the first T cell division.

Journal of immunology (Baltimore, Md. : 1950)

Lemaître F,Moreau HD,Vedele L,Bousso P

25-0081 was used in Flow cytometry/Cell sorting to study the development of heterogeneous populations from single CD8(+) T cell progenies.

Thu Aug 15 00:00:00 EDT 2013

Peptidoglycan recognition protein 1 enhances experimental asthma by promoting Th2 and Th17 and limiting regulatory T cell and plasmacytoid dendritic cell responses.

Journal of immunology (Baltimore, Md. : 1950)

Park SY,Jing X,Gupta D,Dziarski R

25-0081 was used in Flow cytometry/Cell sorting to demonstrate the proinflammatory effect of peptidoglycan recognition protein (Pglyrp)1 during the development of allergic asthma.

Mon Apr 01 00:00:00 EDT 2013

Toll-like receptor agonists and alpha-galactosylceramide synergistically enhance the production of interferon-gamma in murine splenocytes.

Scientific reports

Ando T,Ito H,Ohtaki H,Seishima M

25-0081 was used in Flow cytometry/Cell sorting to explore the effects of the costimulation of GalCer and TLR agonists on the immune system and cytokine production.

Fri Feb 14 00:00:00 EST 2014

The ARNT-STAT3 axis regulates the differentiation of intestinal intraepithelial TCRαβ⁺CD8αα⁺ cells.

Nature communications

Nakajima K,Maekawa Y,Kataoka K,Ishifune C,Nishida J,Arimochi H,Kitamura A,Yoshimoto T,Tomita S,Nagahiro S,Yasutomo K

25-0081 was used in Flow cytometry/Cell sorting to demonstrate that the ARNT-STAT3 axis is a critical regulator of TCRαβ(+)CD8αα(+) intestinal intraepithelial T-cell development and differentiation.

Thu Jan 30 00:00:00 EST 2014

Partial deficiency of sphingosine-1-phosphate lyase confers protection in experimental autoimmune encephalomyelitis.

PloS one

Billich A,Baumruker T,Beerli C,Bigaud M,Bruns C,Calzascia T,Isken A,Kinzel B,Loetscher E,Metzler B,Mueller M,Nuesslein-Hildesheim B,Kleylein-Sohn B

25-0081 was used in Flow cytometry/Cell sorting to investigate the involvement of Sgpl1 in models of disease, showing that partial deficiency confers protection in EAE.

Tue Sep 17 00:00:00 EDT 2013

Activation of CD4+ T lymphocytes improves wound healing and survival after experimental myocardial infarction in mice.

Circulation

Hofmann U,Beyersdorf N,Weirather J,Podolskaya A,Bauersachs J,Ertl G,Kerkau T,Frantz S

25-0081 was used in Flow cytometry/Cell sorting to investigate the role of CD4+ T lymphocytes in wound healing after experimental myocardial infarction in mice.

Tue Apr 03 00:00:00 EDT 2012

Nucleoside salvage pathway kinases regulate hematopoiesis by linking nucleotide metabolism with replication stress.

The Journal of experimental medicine

Austin WR,Armijo AL,Campbell DO,Singh AS,Hsieh T,Nathanson D,Herschman HR,Phelps ME,Witte ON,Czernin J,Radu CG

25-0081 was used in Flow cytometry/Cell sorting to investigate a functional link between nucleotide deficiency, replication stress, and two enzymes of the nucleoside salvage pathway: deoxycytidine kinase and thymidine kinase.

Mon Nov 19 00:00:00 EST 2012

Chemokine receptor Ccr1 drives neutrophil-mediated kidney immunopathology and mortality in invasive candidiasis.

PLoS pathogens

Lionakis MS,Fischer BG,Lim JK,Swamydas M,Wan W,Richard Lee CC,Cohen JI,Scheinberg P,Gao JL,Murphy PM

25-0081 was used in Flow cytometry/Cell sorting to show that mice lacking the chemokine receptor Ccr1 have improved renal function and survival without affecting the tissue fungal burden.

Mon Dec 17 00:00:00 EST 2012

Differential requirements for IRF-2 in generation of CD1d-independent T cells bearing NK cell receptors.

Journal of immunology (Baltimore, Md. : 1950)

Notake T,Horisawa S,Sanjo H,Miyagawa S,Hida S,Taki S

25-0081 was used in Flow cytometry/Cell sorting to investigate the mechanisms behind the generation and function of NKR(+) T cells.

Tue May 15 00:00:00 EDT 2012

STAT1-independent control of a neurotropic measles virus challenge in primary neurons and infected mice.

Journal of immunology (Baltimore, Md. : 1950)

O'Donnell LA,Conway S,Rose RW,Nicolas E,Slifker M,Balachandran S,Rall GF

25-0081 was used in Flow cytometry/Cell sorting to determine whether STAT1 is required for IFN-γ-mediated protection from infection in neurons, showing that absence of STAT1 in mice did not restrict the ability of IFN-γ to control viral infection, through a pathway independent of the usual pathway.

Wed Feb 15 00:00:00 EST 2012

Differential effects of IL-12 on Tregs and non-Treg T cells: roles of IFN-γ, IL-2 and IL-2R.

PloS one

Zhao J,Zhao J,Perlman S

25-0081 was used in Flow cytometry/Cell sorting to investigate whether loss of immunosuppression occurs when IFN-γ is expressed by Tregs, showing differential effects of IL-12 on Tregs and non-Treg T cells.

Thu Feb 21 00:00:00 EST 2013

Negative selection assay based on stimulation of T cell receptor transgenic thymocytes with peptide-MHC tetramers.

PloS one

Rybakin V,Gascoigne NR

25-0081 was used in Flow cytometry/Cell sorting to develop an assay for the induction of negative selection based on stimulation of T cell receptor transgenic thymocytes with peptide-MHC tetramers.

Fri Mar 22 00:00:00 EDT 2013

Proteomic cornerstones of hematopoietic stem cell differentiation: distinct signatures of multipotent progenitors and myeloid committed cells.

Molecular & cellular proteomics : MCP

Klimmeck D,Hansson J,Raffel S,Vakhrushev SY,Trumpp A,Krijgsveld J

25-0081 was used in Flow cytometry/Cell sorting to discern the distinct signatures of multipotent progenitors and myeloid committed cells.

Wed Aug 01 00:00:00 EDT 2012

Lymphoma-like T cell infiltration in liver is associated with increased copy number of dominant negative form of TGFβ receptor II.

PloS one

Zhang W,Tsuda M,Yang GX,Tsuneyama K,He XS,Ansari AA,Ridgway WM,Coppel RL,Lian ZX,Leung PS,Gershwin ME

25-0081 was used in Flow cytometry/Cell sorting to further define the processes underlying the development of lymphoma-like T cell infiltration.

Tue Jun 25 00:00:00 EDT 2013

RUNX transcription factor-mediated association of Cd4 and Cd8 enables coordinate gene regulation.

Immunity

Collins A,Hewitt SL,Chaumeil J,Sellars M,Micsinai M,Allinne J,Parisi F,Nora EP,Bolland DJ,Corcoran AE,Kluger Y,Bosselut R,Ellmeier W,Chong MM,Littman DR,Skok JA

25-0081 was used in Flow cytometry/Cell sorting to study how communication between Cd4 and Cd8 appears to enable long-range epigenetic regulation to ensure that expression of one excludes the other in mature CD4 or CD8 single-positive cells.

Fri Mar 25 00:00:00 EDT 2011

Th17 cells induce ectopic lymphoid follicles in central nervous system tissue inflammation.

Immunity

Peters A,Pitcher LA,Sullivan JM,Mitsdoerffer M,Acton SE,Franz B,Wucherpfennig K,Turley S,Carroll MC,Sobel RA,Bettelli E,Kuchroo VK

25-0081 was used in Flow cytometry/Cell sorting to show that in experimental autoimmune encephalomyelitis Th17 cells specifically induced ectopic lymphoid follicles in the central nervous system.

Fri Dec 23 00:00:00 EST 2011

RelA and RelB transcription factors in distinct thymocyte populations control lymphotoxin-dependent interleukin-17 production in γδ T cells.

Immunity

Powolny-Budnicka I,Riemann M,Tänzer S,Schmid RM,Hehlgans T,Weih F

25-0081 was used in Flow cytometry/Cell sorting to investigate the contribution of NF-κB to IL-17 production by T cells, showing that RelA and RelB in distinct thymocyte populations control lymphotoxin-dependent IL-17 production.

Fri Mar 25 00:00:00 EDT 2011

Inhibitory receptors specific for MHC class I educate murine NK cells but not CD8αα intestinal intraepithelial T lymphocytes.

Blood

Taveirne S,Filtjens J,Van Ammel E,De Colvenaer V,Kerre T,Taghon T,Vandekerckhove B,Plum J,Held W,Leclercq G

25-0081 was used in Functional assays to assess whether inhibitory MHC-I receptors educate only natural killer cells or whether they evoke responses in all cell types, which express them.

Thu Jul 14 00:00:00 EDT 2011

PML-RARA can increase hematopoietic self-renewal without causing a myeloproliferative disease in mice.

The Journal of clinical investigation

Welch JS,Yuan W,Ley TJ

25-0081 was used in Flow cytometry/Cell sorting to study a platform for more accurately dissecting the early events in acute promyelocytic leukemia pathogenesis.

Fri Apr 01 00:00:00 EDT 2011

Ly49D-mediated ITAM signaling in immature thymocytes impairs development by bypassing the pre-TCR checkpoint.

Journal of immunology (Baltimore, Md. : 1950)

Merck E,Lees RK,Voyle RB,Held W,MacDonald HR

25-0081 was used in Flow cytometry/Cell sorting to investigate the role of Ly49D-mediated ITAM signaling, showing that it impairs development by bypassing the pre-TCR checkpoint.

Fri Jul 01 00:00:00 EDT 2011

Cutting edge: attrition of Plasmodium-specific memory CD8 T cells results in decreased protection that is rescued by booster immunization.

Journal of immunology (Baltimore, Md. : 1950)

Schmidt NW,Harty JT

25-0081 was used in Flow cytometry/Cell sorting to investigate whether unrelated infections will compromise numbers of Plasmodium-specific memory CD8 T cells, showing that attrition of Plasmodium-specific cells is rescued by booster immunisation.

Fri Apr 01 00:00:00 EDT 2011

Restricted microbiota and absence of cognate TCR antigen leads to an unbalanced generation of Th17 cells.

Journal of immunology (Baltimore, Md. : 1950)

Lochner M,Bérard M,Sawa S,Hauer S,Gaboriau-Routhiau V,Fernandez TD,Snel J,Bousso P,Cerf-Bensussan N,Eberl G

25-0081 was used in Flow cytometry/Cell sorting to demonstrate that a complex microbiota and cognate antigen are required to generate a properly regulated set of Foxp3+RORγt+ T cells and Th17 cells.

Tue Feb 01 00:00:00 EST 2011

Chronic helminth infection promotes immune regulation in vivo through dominance of CD11cloCD103- dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Smith KA,Hochweller K,Hämmerling GJ,Boon L,MacDonald AS,Maizels RM

25-0081 was used in Flow cytometry/Cell sorting to identify that the preferential expansion of a novel population of CD11c(lo) non-plasmacytoid DCs is associated with the pro-regulatory environment of chronic intestinal helminth infection.

Wed Jun 15 00:00:00 EDT 2011

T cell-intrinsic and -extrinsic contributions of the IFNAR/STAT1-axis to thymocyte survival.

PloS one

Moro H,Otero DC,Tanabe Y,David M

25-0081 was used in Flow cytometry/Cell sorting to demonstrate that type I interferons and STAT1 play an important role in the survival of MHC class I-restricted T cells.

Fri Feb 03 00:00:00 EST 2012

Measles virus infection of alveolar macrophages and dendritic cells precedes spread to lymphatic organs in transgenic mice expressing human signaling lymphocytic activation molecule (SLAM, CD150).

Journal of virology

Ferreira CS,Frenzke M,Leonard VH,Welstead GG,Richardson CD,Cattaneo R

25-0081 was used in Flow cytometry/Cell sorting to identify the cells that support primary measles infection before systemic spread.

Mon Mar 01 00:00:00 EST 2010

Virulence of Toxoplasma gondii is associated with distinct dendritic cell responses and reduced numbers of activated CD8+ T cells.

Journal of immunology (Baltimore, Md. : 1950)

Tait ED,Jordan KA,Dupont CD,Harris TH,Gregg B,Wilson EH,Pepper M,Dzierszinski F,Roos DS,Hunter CA

25-0081 was used in Flow cytometry/Cell sorting to show the dendritic cell response to T.gondii alters levels of CD8+ T cells which ultimately determine the outcome of infection.

Sun Aug 01 00:00:00 EDT 2010

Additive and global functions of HoxA cluster genes in mesoderm derivatives.

Developmental biology

Di-Poï N,Koch U,Radtke F,Duboule D

25-0081 was used in Flow cytometry/Cell sorting to report that mutant mice lacking the entire HoxA cluster in mesodermal lineages display the expected spectrum of postnatal respiratory, cardiac and urogenital defects, previously reported for single gene mutations.

Sat May 15 00:00:00 EDT 2010

Flow cytometry analysis of transcription factors in T lymphocytes.

Methods in molecular biology (Clifton, N.J.)

Albu DI,Califano D,Avram D

25-0081 was used in Flow cytometry/Cell sorting to develop and optimise a protocol for the rapid and convenient detection of the transcription factor BCL11B in T lymphocyte subpopulations.

Tue Dec 28 00:00:00 EST 2010

IL-7 adjuvant treatment enhances long-term tumor-antigen-specific CD8+ T-cell responses after immunization with recombinant lentivector.

Blood

Colombetti S,Lévy F,Chapatte L

25-0081 was used in Flow cytometry/Cell sorting to identify that IL-7 treatment should be beneficial in vaccinations settings in which high frequencies of effector CD8+ T cells do not down-regulate IL-7R expression upon priming, such as recombinant lentivector-based vaccines.

Thu Jun 25 00:00:00 EDT 2009

Distinct roles for MyD88 and Toll-like receptor 2 during Leishmania braziliensis infection in mice.

Infection and immunity

Vargas-Inchaustegui DA,Tai W,Xin L,Hogg AE,Corry DB,Soong L

25-0081 was used in Flow cytometry/Cell sorting to investigate whether TLRs are involved in L. braziliensis-mediated dendritic cell activation, showing distinct roles for MyD88 and TLR2 in infection.

Wed Jul 01 00:00:00 EDT 2009

In vivo equilibrium of proinflammatory IL-17+ and regulatory IL-10+ Foxp3+ RORgamma t+ T cells.

The Journal of experimental medicine

Lochner M,Peduto L,Cherrier M,Sawa S,Langa F,Varona R,Riethmacher D,Si-Tahar M,Di Santo JP,Eberl G

25-0081 was used in Flow cytometry/Cell sorting to study how potentially antagonistic pro-inflammatory IL-17-producing and regulatory Foxp3(+) RORgamma t(+) T cells coexist and are tightly controlled.

Mon Jun 09 00:00:00 EDT 2008

Regulation of cytokine production by virus-specific CD8 T cells in the lungs.

Journal of virology

Fulton RB,Olson MR,Varga SM

25-0081 was used in Flow cytometry/Cell sorting to show that both virus-induced inflammation and the host tissue environment influence the capacity of virus-specific T cells to produce cytokines.

Fri Aug 01 00:00:00 EDT 2008

Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2.

Investigative ophthalmology & visual science

Mochimaru H,Usui T,Yaguchi T,Nagahama Y,Hasegawa G,Usui Y,Shimmura S,Tsubota K,Amano S,Kawakami Y,Ishida S

Thu May 01 00:00:00 EDT 2008

Myocardial infarct-sparing effect of adenosine A2A receptor activation is due to its action on CD4+ T lymphocytes.

Circulation

Yang Z,Day YJ,Toufektsian MC,Xu Y,Ramos SI,Marshall MA,French BA,Linden J

Tue Nov 07 00:00:00 EST 2006

Cytosolic entry controls CD8+-T-cell potency during bacterial infection.

Infection and immunity

Bahjat KS,Liu W,Lemmens EE,Schoenberger SP,Portnoy DA,Dubensky TW,Brockstedt DG

25-0081 was used in Flow cytometry/Cell sorting to investigate microbial invasion of the cytosol as a key factor in inducing CD8+- T cell-mediated protection against L.monocytogenes.

Wed Nov 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Lymphocyte antigen 3; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-cell surface glycoprotein Lyt-3

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Gene Aliases: BB154331; Cd8a; Cd8b; Cd8b1; Ly-2; Ly-3; Ly-35; Ly-B; Ly-C; Lyt-2; Lyt-3; Lyt2; Lyt3

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UniProt ID: (Mouse) P01731, (Mouse) P10300

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Entrez Gene ID: (Mouse) 12525, (Mouse) 12526

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Function(s)

protein binding protein kinase binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

Process(es)

immune system process T cell mediated immunity cell surface receptor signaling pathway T cell activation cytotoxic T cell differentiation positive regulation of calcium-mediated signaling defense response to virus

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CD8a Monoclonal Antibody (53-6.7), PE-Cyanine7, eBioscience™

CD8a Antibody (25-0081-82) in Flow

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25-0081-82

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