Invitrogen

CD16/CD32 Monoclonal Antibody (93), Biotin, eBioscience™

CD16/CD32 Antibody in Flow Cytometry (Flow)

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CD16/CD32 Antibody (13-0161-82) in Flow

Staining of mouse splenocytes with Anti-Mouse CD16/CD32 APC (left), and PE-Cy7 (right). Autofluorescence is shown via open histogram. Total cells were used for analysis.

CD16/CD32 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

Product Image Gallery

Product Details

13-0161-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.125 µg/test

View 14 publications 14 publications

Affinity Purification (AP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Fruit fly, Mouse

Host/Isotype

Rat / IgG2a, lambda

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466377

Product Specific Information

Description: The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.

Applications Reported: The 93 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 93 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD16 is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by natural killer (NK) cells, and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC (antibody dependent cell mediated cytotoxicity) by NK cells. IN comparison, CD32 is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII), and mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Further, CD32 is involved in the phagocytosis of immune complexes, the regulation of antibody production by B-cells, and variations of the CD32 gene may increase vulnerability to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms of CD32 have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nonredundancy of IL-1α and IL-1β is defined by distinct regulation of tissues orchestrating resistance versus tolerance to infection.

Science advances

Eislmayr K,Bestehorn A,Morelli L,Borroni M,Vande Walle L,Lamkanfi M,Kovarik P

Published figure using CD16/CD32 monoclonal antibody (Product # 13-0161-82) in Flow Cytometry

Fri Mar 04 00:00:00 EST 2022

Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions.

The Journal of experimental medicine

Ho TT,Dellorusso PV,Verovskaya EV,Bakker ST,Flach J,Smith LK,Ventura PB,Lansinger OM,Hérault A,Zhang SY,Kang YA,Mitchell CA,Villeda SA,Passegué E

Published figure using CD16/CD32 monoclonal antibody (Product # 13-0161-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

C/EBPα and GATA-2 Mutations Induce Bilineage Acute Erythroid Leukemia through Transformation of a Neomorphic Neutrophil-Erythroid Progenitor.

Cancer cell

Di Genua C,Valletta S,Buono M,Stoilova B,Sweeney C,Rodriguez-Meira A,Grover A,Drissen R,Meng Y,Beveridge R,Aboukhalil Z,Karamitros D,Belderbos ME,Bystrykh L,Thongjuea S,Vyas P,Nerlov C

Published figure using CD16/CD32 monoclonal antibody (Product # 13-0161-82) in Flow Cytometry

Mon May 11 00:00:00 EDT 2020

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Published figure using CD16/CD32 monoclonal antibody (Product # 13-0161-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

Rate of Progression through a Continuum of Transit-Amplifying Progenitor Cell States Regulates Blood Cell Production.

Developmental cell

Li H,Natarajan A,Ezike J,Barrasa MI,Le Y,Feder ZA,Yang H,Ma C,Markoulaki S,Lodish HF

13-0161-81 was used in Flow Cytometry to show that glucocorticoids enhance erythrocyte production by slowing the rate of progression through this developmental continuum of transit-amplifying progenitors, permitting more cell divisions prior to terminal erythroid differentiation.

Mon Apr 08 00:00:00 EDT 2019

miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling.

Nature communications

Lam J,van den Bosch M,Wegrzyn J,Parker J,Ibrahim R,Slowski K,Chang L,Martinez-Høyer S,Condorelli G,Boldin M,Deng Y,Umlandt P,Fuller M,Karsan A

Published figure using CD16/CD32 monoclonal antibody (Product # 13-0161-82) in Flow Cytometry

Wed Jun 20 00:00:00 EDT 2018

Myeloid progenitor cluster formation drives emergency and leukaemic myelopoiesis.

Nature

Hérault A,Binnewies M,Leong S,Calero-Nieto FJ,Zhang SY,Kang YA,Wang X,Pietras EM,Chu SH,Barry-Holson K,Armstrong S,Göttgens B,Passegué E

Published figure using CD16/CD32 monoclonal antibody (Product # 13-0161-82) in Flow Cytometry

Thu Apr 06 00:00:00 EDT 2017

Rational combination of oncolytic vaccinia virus and PD-L1 blockade works synergistically to enhance therapeutic efficacy.

Nature communications

Liu Z,Ravindranathan R,Kalinski P,Guo ZS,Bartlett DL

Published figure using CD16/CD32 monoclonal antibody (Product # 13-0161-82) in Flow Cytometry

Mon Mar 27 00:00:00 EDT 2017

Gliotoxin Suppresses Macrophage Immune Function by Subverting Phosphatidylinositol 3,4,5-Trisphosphate Homeostasis.

mBio

Schlam D,Canton J,Carreño M,Kopinski H,Freeman SA,Grinstein S,Fairn GD

Published figure using CD16/CD32 monoclonal antibody (Product # 13-0161-82) in Flow Cytometry

Tue Apr 05 00:00:00 EDT 2016

Gliotoxin Suppresses Macrophage Immune Function by Subverting Phosphatidylinositol 3,4,5-Trisphosphate Homeostasis.

mBio

Schlam D,Canton J,Carreño M,Kopinski H,Freeman SA,Grinstein S,Fairn GD

Published figure using CD16/CD32 monoclonal antibody (Product # 13-0161-82) in Flow Cytometry

Tue Apr 05 00:00:00 EDT 2016

Agonist anti-GITR monoclonal antibody and stereotactic radiation induce immune-mediated survival advantage in murine intracranial glioma.

Journal for immunotherapy of cancer

Patel MA,Kim JE,Theodros D,Tam A,Velarde E,Kochel CM,Francica B,Nirschl TR,Ghasemzadeh A,Mathios D,Harris-Bookman S,Jackson CC,Jackson C,Ye X,Tran PT,Tyler B,Coric V,Selby M,Brem H,Drake CG,Pardoll DM,Lim M

Published figure using CD16/CD32 monoclonal antibody (Product # 13-0161-82) in Flow Cytometry

Wed May 18 00:00:00 EDT 2016

Immune complexes regulate bone metabolism through FcRγ signalling.

Nature communications

Negishi-Koga T,Gober HJ,Sumiya E,Komatsu N,Okamoto K,Sawa S,Suematsu A,Suda T,Sato K,Takai T,Takayanagi H

13-0161 was used in Flow cytometry/Cell sorting to investigate whether immune complexes regulate bone metabolism directly.

Tue Mar 31 00:00:00 EDT 2015

Morphine decreases bacterial phagocytosis by inhibiting actin polymerization through cAMP-, Rac-1-, and p38 MAPK-dependent mechanisms.

The American journal of pathology

Ninković J,Roy S

Published figure using CD16/CD32 monoclonal antibody (Product # 13-0161-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2012

Morphine decreases bacterial phagocytosis by inhibiting actin polymerization through cAMP-, Rac-1-, and p38 MAPK-dependent mechanisms.

The American journal of pathology

Ninković J,Roy S

Published figure using CD16/CD32 monoclonal antibody (Product # 13-0161-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2012

In vivo inhibition of c-MYC in myeloid cells impairs tumor-associated macrophage maturation and pro-tumoral activities.

PloS one

Pello OM,Chèvre R,Laoui D,De Juan A,Lolo F,Andrés-Manzano MJ,Serrano M,Van Ginderachter JA,Andrés V

Published figure using CD16/CD32 monoclonal antibody (Product # 13-0161-82) in Flow Cytometry

Mon Feb 25 00:00:00 EST 2013

Tracking single hematopoietic stem cells in vivo using high-throughput sequencing in conjunction with viral genetic barcoding.

Nature biotechnology

Lu R,Neff NF,Quake SR,Weissman IL

13-0161 was used in Magnetic cell separation to investigate how to combine viral genetic barcoding with high-throughput sequencing to track single cells in a heterogeneous population.

Sun Oct 02 00:00:00 EDT 2011

The transcriptional coactivator Cbp regulates self-renewal and differentiation in adult hematopoietic stem cells.

Molecular and cellular biology

Chan WI,Hannah RL,Dawson MA,Pridans C,Foster D,Joshi A,Göttgens B,Van Deursen JM,Huntly BJ

13-0161 was used in Flow cytometry/Cell sorting to investigate the role of Cbp in adult HSC maintenance, and the cellular and molecular mechanisms underlying its function.

Thu Dec 01 00:00:00 EST 2011

Ethanol exposure impairs LPS-induced pulmonary LIX expression: alveolar epithelial cell dysfunction as a consequence of acute intoxication.

Alcoholism, clinical and experimental research

Walker JE,Odden AR,Jeyaseelan S,Zhang P,Bagby GJ,Nelson S,Happel KI

13-0161 was used in Affinity chromatography to demonstrate direct suppression of AE2 cell innate immune function by ethanol.

Sun Feb 01 00:00:00 EST 2009

Bioinformatics

Protein Aliases: CD16A; CD16B; CD32; CD32 receptor 2; Fc gamma receptor IIB; Fc gamma receptor III; Fc gamma RIIB; Fc-gamma RII; fc-gamma RIII; Fc-gamma-RII; fc-gamma-RIIB; Fcg receptor III; FCG2; FcgammaRIIB; FcgammaRIII; FCGR2C; FCGR3A; FCGR3B; FCGRIII; FCR-10; FcRII-b; FcRII-c; fcRIII; FCRIIIA; FGFR2B; IgG Fc receptor II beta; igG Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor II; low affinity immunoglobulin gamma Fc region receptor II-b; low affinity immunoglobulin gamma Fc region receptor III; Ly-17; Lymphocyte antigen 17; RP11-5K23.1

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Gene Aliases: AI528646; CD16; CD32; F630109E10Rik; Fc[g]RII; Fcgr2; Fcgr2a; Fcgr2b; FcgRII; Fcr-2; Fcr-3; fcRII; Ly-17; Ly-m20; LyM-1

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UniProt ID: (Mouse) P08101

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Entrez Gene ID: (Mouse) 14130, (Mouse) 14131

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Function(s)

receptor signaling protein activity protein binding IgG binding immunoglobulin binding IgG receptor activity immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD16/CD32 Monoclonal Antibody (93), Biotin, eBioscience™

CD16/CD32 Antibody (13-0161-82) in Flow

Product Details

13-0161-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Affinity Purification (AP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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