PCR Thermal Cyclers Support - Getting Started

MicroAmp 96-Well Tray for VeriFlex Blocks (Cat. No. 4379983) is needed to load 1 or 2 MicroAmp Optical 8-Tube Strips with Attached Optical Caps on the ProFlex, SimpliAmp, MiniAmp, MiniAmp Plus, and Veriti Thermal Cycler 96-well blocks, and QuantStudio 3 and QuantStudio 5 blocks.

The MicroAmp Optical 8-Tube Strip with Attached Optical Caps is compatible with the following instruments:

- ProFlex 3 x 32-well PCR System
- ProFlex 96-well PCR System
- Veriti 96-well Thermal Cycler
- SimpliAmp Thermal Cycler
- MiniAmp Thermal Cycler
- MiniAmp Plus Thermal Cycler
- 2720 Thermal Cycler
- GeneAmp PCR System 9700, 96-well
- 7000 PCR System
- 7300 Real-Time PCR System
- 7500 Real-Time PCR system
- QuantStudio Real-Time PCR Systems
- ViiA7 Real-Time PCR System, 96-well

The MicroAmp 8-Tube Strip with Attached Domed Caps is compatible with the following instruments:

- ProFlex 3 x 32-well PCR System
- ProFlex 96-well
- Veriti 96-well Thermal Cycler
- SimpliAmp Thermal Cycler
- MiniAmp Thermal Cycler
- MiniAmp Plus Thermal Cycler
- 2720 Thermal Cycler
- GeneAmp PCR System 9700, 96-well

MicroAmp 96-Well Tray for VeriFlex Blocks (Cat. No. 4379983) is needed to load 1 or 2 MicroAmp Optical 8-Tube Strips with Attached Domed Caps on the ProFlex, SimpliAmp, MiniAmp, MiniAmp Plus, and Veriti Thermal Cycler 96-well blocks.

Yes. The tubes are DNase/RNase/Human DNA free, as they are manufactered in a Class 100K ISO certified clean room UK production facility.

Yes. The tubes are DNase/RNase/Human DNA free, as they are manufactered in a Class 100K ISO certified clean room UK production facility.

These new strips have new features including a graduated 20 μL measuring mark for visual verification and dual end tabs for better labeling and handling. In addition, the individual caps help prevent cross contamination and reduce sample evaporation.

These new strips have new features including a graduated 20 μL measuring mark for visual verification and dual end tabs for better labeling and handling. In addition, the individual caps help prevent cross contamination and reduce sample evaporation.

Please refer to the table on Page 2 of this flyer for compatibility information.

Please refer to the table on Page 2 of this flyer for compatibility information.

Yes. The tubes are DNase/RNase/Human DNA free, as they are manufactered in a Class 100K ISO certified clean room UK production facility.

Yes. The tubes are DNase/RNase/Human DNA free, as they are manufactered in a Class 100K ISO certified clean room UK production facility.

These new strips have new features including a graduated 20 μL measuring mark for visual verification and dual end tabs for better labeling and handling. In addition, the individual caps help prevent cross contamination and reduce sample evaporation.

These new strips have new features including a graduated 20 μL measuring mark for visual verification and dual end tabs for better labeling and handling. In addition, the individual caps help prevent cross contamination and reduce sample evaporation.

Yes. You can use the MicroAmp Clear Adhesive film (Cat. No. 4306311) on the TriFlex 3 x 32-well plate.

The MicroAmp TriFlex 3 x 32-well plates are not validated for use with real-time PCR (qPCR) 96-well block instruments.

No, side tabs can be left in contact and placed directly on the block when the full plate is used. The tear tabs will not interfere with the fit inside the block.

No. We recommend just putting the MicroAmp TriFlex 32-well plate directly on the ProFlex 32-well block. Do not use the 32-well block frame.

No. The MicroAmp TriFlex 3 x 32-well plate is designed for standard (0.2 mL) 96-well blocks only.

We offer only a 96-well standard (0.2 mL) block that is not interchangeable. The 96-well block for the MiniAmp Plus Thermal Cycler has a block with 3 VeriFlex zones while the MiniAmp Thermal Cycler has an isothermal block.

The compatible plates and plastics are the same as those used with the ProFlex 96-Well PCR System and can be viewed here.

The maximum block ramp rate of the MiniAmp Thermal Cycler is 3 degrees C/sec.
The maximum block ramp rate of the MiniAmp Plus Thermal Cycler is 3.5 degrees C/sec.

The Auto Delta feature allows you to incrementally increase or decrease the temperature each cycle at a step within a cycling stage. One common application for this is touchdown PCR.

The MiniAmp Plus Thermal Cycler features innovative VeriFlex Blocks for enhanced PCR functionality. Three separate Peltier blocks (4 columns each) provide maximal versatility and flexibility and offer two key benefits:

- PCR optimization: each block can be set with a specific temperature, which is ideal for precise control over PCR optimization.
- Run more experiments: the three Peltier blocks can also be used to run up to three different annealing temperatures in the same run.

To add an infinite hold, select the infinity symbol for program.

Like the ProFlex PCR System and SimpliAmp Thermal Cycler, the MiniAmp/MiniAmpPlus Thermal Cycler can be connected wirelessly with an optional High-Power USB W-Fi Module, Cat. No. A26774.

There are 2 key differences:
- The MiniAmp Plus Thermal Cycler has 3 VeriFlex zones while the MiniAmp Thermal Cycler does not.
- The MiniAmp Plus Thermal Cycler has a block ramp rate of 3.5 degrees C/sec while the MiniAmp Thermal Cycler has a block ramp rate of 3.0 degrees C/sec.

The SimpliAmp™ PCR machine can be used with a 4°C hold. Further, our in-house QC testing requires the passing of a “no leak” test make sure that any accumulated water (due to condensation when the block temperature is set to low temperature for a long period of time) will not leak into the instrument causing electronic failure.

The storage capacity for the SimpliAmp™ Thermal Cycler is ~5.9 GB. The run methods and run records stored use most of the memory. It can store 1.2 million run methods.

The two different types of trays are for use with different tubes:

-MicroAmp 96-well Tray/Retainer Set for Veriti Systems (Cat. No. 4381850) - this tray is for use with 0.2 mL single tubes without caps (Cat. Nos. N8010533, N8011533), 0.2 mL 8-tube strip with attached dome caps (Cat. No. A30589), and 0.2 mL tube strips without caps (Cat. No. 4316567)
-MicroAmp 96-well Tray for VeriFlex Blocks (Cat. No. 4379983) - this tray is for use with 0.2 mL single tubes with caps (Cat. Nos. N8010540, N8011540)

We offer only a 96-well standard (0.2 mL) block that is not interchangeable.

The compatible plates and plastics are the same as those used with the ProFlex™ 96-Well PCR System and can be viewed here.

The maximum block ramp rate of the SimpliAmp™ PCR machine is 4°C/sec.

The Auto Delta feature allows you to incrementally increase or decrease the temperature each cycle at a step within a cycling stage. One common application for this is touchdown PCR.

The SimpliAmp™ thermocycler features innovative VeriFlex™ Blocks for enhanced PCR functionality. Three separate Peltier blocks (4 columns each) provide maximal versatility and flexibility and offer two key benefits:

• PCR optimization: each block can be set with a specific temperature, which is ideal for precise control over PCR optimization.
• Run more experiments: the three Peltier blocks can also be used to run up to three different annealing temperatures in the same run.

To add an infinite hold, select the infinity (∞) symbol for program.

Like the ProFlex™ PCR System, the SimpliAmp™ Thermal Cycler can be connected wirelessly with an optional High-Power USB W-Fi Module, Cat. No. A26774.

The ProFlex™ PCR machine has 4 different blocks that can be changed with the flip of a switch, including the first-of-its-kind 3 x 32-well independent block. This block allows up to 3 experiments to be run simultaneously, completely independently of each other and without interference. A dual 96-well block is available for your high throughput sequencing needs and a dual flat block is available to support OpenArray® plate technology for genotyping analysis on the QuantStudio™ 12K Flex Real-Time PCR System.

The plates and plastics will be specific to the block type. Please refer to the tables here for a list of compatible plastics, based on your block type.

The 96-well and 3 x 32-well system have a max block ramp rate of 6.0°C/sec and the dual 96-well and dual flat blocks have a max block ramp rate of 2.0°C/sec.

The Auto Delta feature allows you to incrementally increase or decrease the temperature each cycle at a step within a cycling stage. One common application for this is touchdown PCR.

In the ProFlex™ PCR thermocycler, the 96-well and 3 x 32-well blocks feature innovative VeriFlex™ Blocks for enhanced PCR functionality. Six separate Peltier blocks (2 on each 32-well block for 3 x 32-well) provide maximal versatility and flexibility and offer two key benefits:

1. PCR optimization: each block can be set with a specific temperature, which is ideal for precise control over PCR optimization.
2. Run more experiments: the six Peltier blocks can also be used to run up to six different annealing temperatures in the same run.

To add an infinite hold, select the infinity (∞) symbol for program.

The ProFlex™ PCR System can be connected wirelessly with an optional High-Power USB Wi-Fi Module, Cat. No. A26774. Please note that you will need to update to the latest version of firmware in order to use this Wi-Fi module.

The Veriti® thermocycler is available in four formats:
Veriti™ 96-Well Thermal Cycler
Veriti™ 384-Well Thermal Cycler
Veriti™ 60-Well Thermal Cycler
Veriti™ 96-Well Fast Thermal Cycler

The plates and plastics will be specific to the block type. Choose the appropriate block type below for a list of the compatible tubes, plates, and films.

• Veriti® 60-Well
• Veriti® 96-Well 0.1 mL
• Veriti® 96-Well 0.2 mL
• Veriti® 384-Well

The Auto Delta feature allows you to incrementally increase or decrease the temperature each cycle at a step within a cycling stage. One common application for this is touchdown PCR.

The Veriti® thermocycler with a 96-well block (Fast and Standard) features innovative VeriFlex™ Blocks for enhanced PCR functionality. Six separate Peltier blocks provide maximal versatility and flexibility and offer two key benefits:

1. PCR optimization: each block can be set with a specific temperature, which is ideal for precise control over PCR optimization.
2. Run more experiments: the six Peltier blocks can also be used to run up to six different annealing temperatures in the same run.

To add an infinite hold, select the infinity (∞) symbol for program.

Please refer to the guidelines in this protocol specific to the Veriti® PCR machine.

We currently offer only a 96-well, 0.2 mL (standard) VeriFlex block that is not interchangeable.

The compatible plates and plastics are the same as those used with the ProFlex 96-Well PCR System and can be viewed here: https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics/thermal-cycler-plastics/proflex-96-3x32.html.

The maximum block ramp rate for the VeritiPro Thermal Cycler is 6.0 degrees C/sec.

The AutoDelta feature allows you to incrementally increase or decrease the temperature or hold time with each cycle for a given step within a cycling stage. One common application for this is touchdown PCR.

The VeritiPro Thermal Cycler features VeriFlex blocks for enhanced PCR functionality. Six separate Peltier blocks (6 columns each) provide maximal versatility and flexibility and offer two key benefits:

- PCR optimization: Each block can be set with a specific temperature, which is ideal for precise control over PCR optimization.
- Run more experiments: The three Peltier blocks can also be used to run up to six different annealing temperatures in the same run.

To add an infinite hold on the VeritiPro Thermal Cycler, select the infinity symbol for program.

The VeritiPro Thermal Cycler can be connected wirelessly with an optional High-Power USB W-Fi Module, Cat. No. A26774.

Please see the list here for compatible plates and tubes.

The max block ramp rate of the 2720 PCR machine is 2.3°C/sec.

<p>The Auto Delta feature allows you to incrementally increase or decrease the temperature each cycle at a step within a cycling stage. One common application for this is touchdown PCR.</p>

No, the 2720 PCR machine does not have a gradient feature. Our newer thermal cyclers including the ProFlex™ PCR System and Veriti® Thermal Cycler have better-than-gradient VeriFlex™ blocks.

To add an infinite hold, set the time for the hold to ‘99.59’. The screen should then default to the infinity (∞) symbol for your hold.

Please refer to the guidelines in this protocol specific to the 2720 thermocycler.

The GeneAmp® PCR System 9700 offers interchangeable sample blocks in a variety of well formats and materials to accommodate a wide range of applications.

• 96-Well Aluminum
• 96-Well Gold
• 96-Well Silver
• Dual 96-Well
• Dual 384-Well
• 60-Well

The plates and plastics will be specific to the block type. Please refer to the tables here for a list of compatible plastics, based on your block type.

The Auto Delta feature allows you to incrementally increase or decrease the temperature each cycle at a step within a cycling stage. One common application for this is touchdown PCR.

No, the GeneAmp® PCR System 9700 does not have a gradient feature. Our newer thermal cyclers including the ProFlex™ PCR System and Veriti® Thermal Cycler have better-than-gradient VeriFlex™ Blocks.

To add an infinite hold, set the time for the hold to ‘99.59’. The screen should then default to the infinity (∞) symbol for your hold.

Please refer to the guidelines in this protocol specific to the GeneAmp® PCR System 9700 and block type.