| Probe Type | Application | Starting Material for Probe Synthesis | Probe Synthesis Reaction | Ambion Kit | Comments |
|---|---|---|---|---|---|
| dsDNA (I,N) | Northern, Southern, dot blots | cDNA inserts, PCR product, plasmid with insert | Random priming reaction | DECAprime™ II |
|
| ssRNA (I,N) | NPA, in situ hybridization | Plasmid with insert and polymerase promoter | In vitro transcription | MAXIscript™ |
|
| Oligonucleotide (I) | Northern, Southern, dot blots | Oligonucleotide | End labeling | KinaseMax™ |
|
| NPA | Oligonucleotide | End labeling | KinaseMax™ |
| |
| ssRNA ssDNA dsDNA Oligonucleotide (N) | Northern, Southern, Dot blots, in situ hybridization | DNA, RNA or oligonucleotide | Chemical labeling, post-nucleic acid synthesis | BrightStar™ Psoralen-Biotin |
|
(I) Isotopic
(N) Nonisotopic