Bacterial Artificial Chromosomes (BAC) have been developed to accommodate much larger pieces of DNA than plasmids can. To provide maximum information and utility, a BAC library is generally organized into a map that reflects the DNA sequence in a chromosome. The high-throughput BAC fingerprinting screening using the Prism® SNaPshot® Multiplex Kit is an effective, easy, and cost-efficient solution that has been validated on the Life Technologies 3730/3730xl DNA Analyzers to map BAC libraries.
Step-by-Step Guide to BAC Fingerprinting
 DNA extraction is a critical first step in the experimental workflow of DNA Sequencing and Fragment analysis. The overall quality, accuracy and length of the DNA sequence read can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction. Recommended Product: DNA Isolation |
The restriction ends are labeled by incorporation of a fluorescently-labeled dideoxynucleotide complementary to the informatory first base at the 3' end. Recommended Products: Perform Restriction Digestion |
Recommended Products: Perform and purify the SNaPshot® reaction
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 Add aliquot of PCR product to size standard and Hi-Di Formamide mix; heat denature and proceed with electrophoresis. Recommended Products: Prepare Sample for AnalysisSizing InformationTo learn more about our GeneScan Size standards used in sizing experiments please see our table below.
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 During capillary electrophoresis, the products of the PCR are injected electrokinetically into capillaries filled with polymer. High voltage is applied so that the fluorescent DNA fragments are separated by size and are detected by a laser/camera system. Which Electrophoresis Instrument (Genetic Analyzer) Is Right for You? |
 Analyze the BAC fingerprint profile. Which Data Analysis Software Is Right for You? |
DNA extraction is a critical first step in the experimental workflow of DNA Sequencing and Fragment analysis. The overall quality, accuracy and length of the DNA sequence read can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction. Recommended Product: DNA Isolation |
The restriction ends are labeled by incorporation of a fluorescently-labeled dideoxynucleotide complementary to the informatory first base at the 3' end. Recommended Products: Perform Restriction Digestion |
Recommended Products: Perform and purify the SNaPshot® reaction
| ||||||||||
Add aliquot of PCR product to size standard and Hi-Di Formamide mix; heat denature and proceed with electrophoresis. Recommended Products: Prepare Sample for AnalysisSizing InformationTo learn more about our GeneScan Size standards used in sizing experiments please see our table below.
|
During capillary electrophoresis, the products of the PCR are injected electrokinetically into capillaries filled with polymer. High voltage is applied so that the fluorescent DNA fragments are separated by size and are detected by a laser/camera system. Which Electrophoresis Instrument (Genetic Analyzer) Is Right for You? |
Analyze the BAC fingerprint profile. Which Data Analysis Software Is Right for You? |
Step-by-Step Guide to BAC Fingerprinting
DNA extraction is a critical first step in the experimental workflow of DNA Sequencing and Fragment analysis. The overall quality, accuracy and length of the DNA sequence read can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction. Recommended Product: DNA Isolation |
The restriction ends are labeled by incorporation of a fluorescently-labeled dideoxynucleotide complementary to the informatory first base at the 3' end. Recommended Products: Perform Restriction Digestion |
Recommended Products: Perform and purify the SNaPshot® reaction
| ||||||||||
Add aliquot of PCR product to size standard and Hi-Di Formamide mix; heat denature and proceed with electrophoresis. Recommended Products: Prepare Sample for AnalysisSizing InformationTo learn more about our GeneScan Size standards used in sizing experiments please see our table below.
|
During capillary electrophoresis, the products of the PCR are injected electrokinetically into capillaries filled with polymer. High voltage is applied so that the fluorescent DNA fragments are separated by size and are detected by a laser/camera system. Which Electrophoresis Instrument (Genetic Analyzer) Is Right for You? |
Analyze the BAC fingerprint profile. Which Data Analysis Software Is Right for You? |
DNA extraction is a critical first step in the experimental workflow of DNA Sequencing and Fragment analysis. The overall quality, accuracy and length of the DNA sequence read can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction. Recommended Product: DNA Isolation |
The restriction ends are labeled by incorporation of a fluorescently-labeled dideoxynucleotide complementary to the informatory first base at the 3' end. Recommended Products: Perform Restriction Digestion |
Recommended Products: Perform and purify the SNaPshot® reaction
| ||||||||||
Add aliquot of PCR product to size standard and Hi-Di Formamide mix; heat denature and proceed with electrophoresis. Recommended Products: Prepare Sample for AnalysisSizing InformationTo learn more about our GeneScan Size standards used in sizing experiments please see our table below.
|
During capillary electrophoresis, the products of the PCR are injected electrokinetically into capillaries filled with polymer. High voltage is applied so that the fluorescent DNA fragments are separated by size and are detected by a laser/camera system. Which Electrophoresis Instrument (Genetic Analyzer) Is Right for You? |
Analyze the BAC fingerprint profile. Which Data Analysis Software Is Right for You? |
Highlighted products for BAC fingerprinting:
For Research Use Only. Not for use in diagnostic procedures.