Dynabeads® Magnetic Beads can be used to isolate a number of different organelles and organelle-specific proteins. This magnetic separation technology enables the specific isolation of true fractions in small volumes, and can replace time-consuming and labor-intensive density gradient centrifugations.
Advantages include:
- Rapid, high-purity isolations from crude fractions or fractions of common densities
- Morphologically intact target organelles can be fixed for EM studies or solubilized directly on the bead surface
- Immunomagnetically isolated organelles can be assayed by conventional techniques
- Easy protocol yields high-purity functional organelles
As more and more organelle-specific markers are identified, the corresponding immunomagnetic isolation of subgroups of organelles can be performed. Examples of organelles or organelle fractions that have been isolated with Dynabeads® include exosomes, mitochondria, peroxisomes, kidney glomeruli, and endocytic and secretory compartments.
Compare Dynabeads® organelle isolation methods
| 1. Select your application | Application specification | 2. Click on the Solution | Advantage |
|---|---|---|---|
| Isolation of fragile organelles or organelle fractions in general | Using a mouse primary antibody | Dynabeads® M-280 Sheep anti-Mouse IgG | Direct or indirect isolation of organelles made more efficient with two layers of Abs. |
| Using a rabbit primary antibody | Dynabeads® M-280 Sheep anti-Rabbit IgG | Direct or indirect isolation of organelles made more efficient with two layers of Abs. | |
| Antibodies made in any other species | Dynabeads® Protein G Dynabeads® Protein A | Efficient direct or indirect isolation of organelles. | |
| Isolation of large organelle fractions or isolation from a viscous sample | Using antibodies made in a specific species | Dynabeads® M-450 with a secondary antibody against the corresponding primary Ab species | More efficient pull to the magnet in viscous samples |
| Isolation of organelles using a ligand that is not an antibody | When high yield is important | Dynabeads® M-280 Tosylactivated | Easy coupling of a molecule onto these hydrophobic beads for efficient organelle isolation |
| Isolation of organelles using a ligand that is not an antibody | When high purity is important | Dynabeads® M-270 Epoxy | Coupling of a molecule onto these hydrophilic beads for very high purity organelle isolation |
Note: 4.5 µm Dynabeads® Magnetic Beads can be used instead
For Research Use Only. Not for use in diagnostic procedures.