Download: Protein Gel Guide
The XCell4 SureLock Midi-Cell Electrophoresis System's unique design allows you to quickly and easily perform simultaneous vertical electrophoresis of 1-4 midi-gels with consistent performance. The XCell4 SureLock Midi-Cell is built with tough polycarbonate to boost durability and features a specially designed lid to enhance user safety.
XCell4 SureLock Midi-Cell specifications
| Capacity | Up to 4 midi gels |
| Gel compatibility | Gel size: 8 x 13cm Gel cassette: 10.3 x 15cm Thickness: 1.0mm |
| Compatible gels | All Invitrogen precast midi gels, and Invitrogen empty midi gel cassettes |
| Buffer chamber Requirement | Upper chamber: 175 mL (per gel) Lower chamber: 700 mL (with 4 gels) |
| Compatible power supplies | PowerEase 300, Zoom Dual Power, Owl systems, for other systems such as Hoefer or Bio-Rad use with Novex Power Supply Adapters (Cat. No. ZA10001) |
| Unit dimensions | 21 x 19 x 16 cm (height with lid on) |
| Material | Polycarbonate |
| Electrode wire | Platinum |
| Electrode limits | 600 VDC or 200 Watts |
| Chemical resistance | Impervious to most alcohols but not compatible with chlorinated hydrocarbons (e.g., chloroform), aromatic hydrocarbons (e.g., toluene, benzene), or acetone. |
| Catalog number | WR0100 |
XCell4 SureLock Midi-Cell system overview
The XCell4 SureLock Midi-Cell Electrophoresis System consists of a Lower Buffer Chamber, two Buffer Cores, Buffer Dam, Displacement Dam, and a lid with cables.
Electrophoresis of proteins on midi-gels in the XCell4 SureLock Midi-Cell is performed after assembling each Buffer Core with 2 midi gels or a Buffer Core with a midi-gel and a Buffer Dam. The Buffer Core/gel assembly is inserted into the Lower Buffer Chamber and is sealed using a positive locking mechanism that seals the Buffer Core/gel assembly firmly into position and ensures leak-free electrophoresis.
The Buffer Core/gel assembly creates separate upper buffer chambers for each gel and a common lower buffer chamber shared by all gels. The lid is placed onto the Midi-Cell and fits on the Midi-Cell in only one orientation to prevent incorrect connection to the electrodes. The unique design of the XCell4 SureLock Midi-Cell evenly dissipates heat resulting in consistent performance of all gels without the need for any external cooling device. The Buffer Core electrical poles are color-coded for easy, intuitive assembly.
Key features of the XCell4 SureLock Midi-Cell Electrophoresis System:
- User-friendly design without any clamps for leak-free electrophoresis
- Flexibility to perform electrophoresis of 1-4 midi-gels
- Unique, heat-dissipating design of the XCell4 SureLock Midi-Cell produces consistent results without the need for any cooling device
- Built-in safety features to enhance user safety
 |
Schematic of XCell4 SureLock Midi-Cell Electrophoresis Unit.
Recommended run conditions
| Gel Type | Voltage (V) | Current* (mA) | Ending Current* | Run Time |
|---|---|---|---|---|
| Tris-glycine SDS-PAGE | 125 | 40-50 | 20-25 | 105 min |
| Tris-glycine Native | 125 | 35-40 | 15-20 | 1-12 hr |
| Bis-Tris (MES) | 200 | 160-200 | 120-170 | 40 min |
| Bis-Tris (MOPS) | 200 | 160-200 | 120-170 | 55 min |
| Tris-acetate SDS-PAGE | 150 | 70-90 | 50-60 | 70 min |
| Tris-acetate Native | 150 | 40-45 | 15-20 | 2-3 hr |
*Current readings are per gel
If simultaneously running 4 gels using the XCell4 SureLock Midi-Cell, we recommend using the ZOOM Dual Power Supply or PowerEase 300. The Low Voltage/High Current outlets can handle the voltage and current of four Bis-Tris gels (Voltage 200 V, Current ~800 mA). The PowerEase 90W Power Supply can be used with the XCell4 SureLock Midi-Cell but the current limit of 500 mA will be reached with 2 gels (2 x ~200 mA max current at 200V). If 4 gels are run in the XCell4 SureLock Midi-Cell with these power supplies, the current will be maxed out and the run will slow down. It will take approximately twice as long to run the gels. Note: To allow connection of the covered power leads of the XCell4 SureLock Midi-Cell with certain power supplies that are not designed for use with covered or non-retractable power leads, use Invitrogen Power Supply Adapters available separately (Cat. No. ZA10001).
XCell4 SureLock Midi-Cell specifications
| Capacity | Up to 4 midi gels |
| Gel compatibility | Gel size: 8 x 13cm Gel cassette: 10.3 x 15cm Thickness: 1.0mm |
| Compatible gels | All Invitrogen precast midi gels, and Invitrogen empty midi gel cassettes |
| Buffer chamber Requirement | Upper chamber: 175 mL (per gel) Lower chamber: 700 mL (with 4 gels) |
| Compatible power supplies | PowerEase 300, Zoom Dual Power, Owl systems, for other systems such as Hoefer or Bio-Rad use with Novex Power Supply Adapters (Cat. No. ZA10001) |
| Unit dimensions | 21 x 19 x 16 cm (height with lid on) |
| Material | Polycarbonate |
| Electrode wire | Platinum |
| Electrode limits | 600 VDC or 200 Watts |
| Chemical resistance | Impervious to most alcohols but not compatible with chlorinated hydrocarbons (e.g., chloroform), aromatic hydrocarbons (e.g., toluene, benzene), or acetone. |
| Catalog number | WR0100 |
XCell4 SureLock Midi-Cell system overview
The XCell4 SureLock Midi-Cell Electrophoresis System consists of a Lower Buffer Chamber, two Buffer Cores, Buffer Dam, Displacement Dam, and a lid with cables.
Electrophoresis of proteins on midi-gels in the XCell4 SureLock Midi-Cell is performed after assembling each Buffer Core with 2 midi gels or a Buffer Core with a midi-gel and a Buffer Dam. The Buffer Core/gel assembly is inserted into the Lower Buffer Chamber and is sealed using a positive locking mechanism that seals the Buffer Core/gel assembly firmly into position and ensures leak-free electrophoresis.
The Buffer Core/gel assembly creates separate upper buffer chambers for each gel and a common lower buffer chamber shared by all gels. The lid is placed onto the Midi-Cell and fits on the Midi-Cell in only one orientation to prevent incorrect connection to the electrodes. The unique design of the XCell4 SureLock Midi-Cell evenly dissipates heat resulting in consistent performance of all gels without the need for any external cooling device. The Buffer Core electrical poles are color-coded for easy, intuitive assembly.
Key features of the XCell4 SureLock Midi-Cell Electrophoresis System:
- User-friendly design without any clamps for leak-free electrophoresis
- Flexibility to perform electrophoresis of 1-4 midi-gels
- Unique, heat-dissipating design of the XCell4 SureLock Midi-Cell produces consistent results without the need for any cooling device
- Built-in safety features to enhance user safety
|
Schematic of XCell4 SureLock Midi-Cell Electrophoresis Unit.
Recommended run conditions
| Gel Type | Voltage (V) | Current* (mA) | Ending Current* | Run Time |
|---|---|---|---|---|
| Tris-glycine SDS-PAGE | 125 | 40-50 | 20-25 | 105 min |
| Tris-glycine Native | 125 | 35-40 | 15-20 | 1-12 hr |
| Bis-Tris (MES) | 200 | 160-200 | 120-170 | 40 min |
| Bis-Tris (MOPS) | 200 | 160-200 | 120-170 | 55 min |
| Tris-acetate SDS-PAGE | 150 | 70-90 | 50-60 | 70 min |
| Tris-acetate Native | 150 | 40-45 | 15-20 | 2-3 hr |
*Current readings are per gel
If simultaneously running 4 gels using the XCell4 SureLock Midi-Cell, we recommend using the ZOOM Dual Power Supply or PowerEase 300. The Low Voltage/High Current outlets can handle the voltage and current of four Bis-Tris gels (Voltage 200 V, Current ~800 mA). The PowerEase 90W Power Supply can be used with the XCell4 SureLock Midi-Cell but the current limit of 500 mA will be reached with 2 gels (2 x ~200 mA max current at 200V). If 4 gels are run in the XCell4 SureLock Midi-Cell with these power supplies, the current will be maxed out and the run will slow down. It will take approximately twice as long to run the gels. Note: To allow connection of the covered power leads of the XCell4 SureLock Midi-Cell with certain power supplies that are not designed for use with covered or non-retractable power leads, use Invitrogen Power Supply Adapters available separately (Cat. No. ZA10001).
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