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Digital and downloadable resources to help with your qPCR research

As veterans and innovators in real-time PCR (qPCR), we have a wealth of information, both technical and educational, to empower your qPCR research. We’ve featured some of the most frequented resources for both master mixes and general qPCR below.


TaqMan & SYBR assays

Learn about the differences between TaqMan and SYBR real-time PCR assays and find out which chemistry is right for your qPCR application.

Choosing the right dyes for your multiplexing experiment

Tips from the field - our own Field Application Scientist answers frequently asked questions regarding dye selection.

qPCR handbook

Whether you’re new to real-time PCR or just looking to brush up on your techniques, this go-to educational resource provides guidance on running a qPCR experiment, how-to videos, diagrams, frequently asked questions, and best practices.

TaqTalk video series

As experts in qPCR, we address your most frequently asked questions and challenges in our video series, Taq Talk, where you can expect to hear anything from qPCR basics and best practices to experiment setup and choosing the right master mix.


TaqMan master mixes product literature

TaqMan Fast Advanced Master Mix

Product bulletin: TaqMan Fast Advanced Master Mix

 

User guide: TaqMan Fast Advanced Master Mix

 

Datasheet: TaqMan Advanced Master Mix on the
Bio-Rad CFX96 Real-Time PCR System

 

Datasheet: TaqMan Advanced Master Mix on the
Roche LightCycler 480 Real-Time PCR System

   

TaqMan Fast Virus 1-Step Master Mix

Product bulletin: TaqMan Fast Virus 1-Step Master Mix

 

User guide: TaqMan Fast Virus 1-Step Master Mix

 

Product insert: TaqMan Fast Virus 1-Step Master Mix

   

TaqMan RNA-to-Ct 1-Step Kit

Product bulletin: TaqMan RNA-to-Ct 1-Step Kit

 

Application note: Understanding Ct

 

Quick reference card: TaqMan RNA-to-Ct 1-Step Kit

   

TaqMan Sample-to-SNP Kit

Product bulletin: TaqMan Sample-to-SNP Kit

 

Quick reference card: TaqMan Sample-to-SNP Kit


SYBR Green master mixes product literature

SYBR Green Master Mixes

White paper: Design and Optimization of SYBR Green Assays

   

PowerTrack SYBR Green Master Mix

Product bulletin: PowerTrack SYBR Green Master Mix

 

Protocol: Guidelines for comparing PowerTrack SYBR Green Master Mix to other SYBR master mixes

   

PowerUp SYBR Green Master Mix

Product bulletin: PowerUp SYBR Green Master Mix

 

Protocol: Guidelines for comparing PowerUp SYBR Green Master Mix to other SYBR master mixes

   

Power SYBR Green RNA-to-Ct 1-Step Kit

Product bulletin: Power SYBR Green RNA-to-Ct 1-Step Kit

 

Application note: Understanding Ct

 

Quick reference card: Power SYBR Green RNA-to-Ct 1-Step Kit

   

High Resolution Melting (HRM)

Product bulletin: MeltDoctor High Resolution Melt Reagents

 

Guide: A Guide to High Resolution Melting (HRM) Analysis

 

Quick reference card: HRM Genotyping Experiment

 

Quick reference card: HRM Methylation Study

 

Quick reference card: HRM Mutation Scanning Experiment

 

Application note: High Resolution Melt Analysis for Mutation Scanning Prior to Sequencing

 

Application note: Methylation Analysis Using Methylation- Sensitive HRM and DNA Sequencing

 

Application note:  High Resolution Melting for Genotyping Applications

 

Application note: High Resolution melt for genotyping in Jatropha plants

 

Customer story: How Dr. Birgit Ebner identifies variants by HRM in difficult samples


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