Sample preparation plays a critical role in producing quality data in your flow cytometry experiment. This section offers educational resources for sample preparation for intracellular staining, extracellular staining and more.
Sample preparation features
Selection guide
Intracellular staining guide (for mouse & human cytokines and non-cytokine proteins)
This selection guide features buffer conditions, blocking conditions and more for select mouse and human protein antigens.
Biology overview
Foxp3: The master regulator of T regulatory cells
This biology overview of Foxp3 includes the challenges in sample preparation when studying this system.
Sample preparation learning resources
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| Type | Title | Categories |
|---|---|---|
| BioProbes articles (Issues 50–present day) | BioProbes Journal of Cell Biology Application | cell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification |
| Immunology information | Foxp3 overview | flow cytometry, Foxp3, intracellular staining |
| Protocols | Cell preparation for flow cytometry protocols (Invitrogen eBioscience reagents) | flow cytometry, sample preparation |
| Protocols | Red blood cell lysis protocols using eBioscience lysis buffers (Invitrogen eBioscience reagents) | flow cytometry, sample preparation |
| Protocols | Staining cell surface targets for flow cytometry (Invitrogen eBioscience reagents) | extracellular staining, flow cytometry, immunophenotyping |
| Protocols | Staining intracellular antigens for flow cytometry (Invitrogen eBioscience reagents) | flow cytometry, immunophenotyping, intracellular staining |
| Protocols | Super Bright staining buffer protocol | antibody conjugates, flow cytometry, immunophenotyping |
| Scientific poster (2009) | A novel xeno-free and serum-free culture medium for the expansion of human mesenchymal stem cells | cell culture, cell expansion, flow cytometry, fluorescent dyes, gene expression, immunophenotyping, stem cell research |
| Scientific poster (2009) | A simple, fast and quantitative single-step dead-cell indicator for flow cytometry | apoptosis, fluorescent dyes, multicolor flow cytometry, nucleic acid quantitation, viability |
| Scientific poster (2009) | Dead cell stains in flow cytometry: A comprehensive analysis | apoptosis, cells, flow cytometry, fluorescent dyes, immunophenotyping, viability |
| Selection guide | Antibody fixation considerations | sample preparation |
| Selection guide | Human antibody cross reactivity chart | antibody cross-reactivity, flow cytometry |
| Selection guide | Mouse antibody cross reactivity chart | antibody cross-reactivity, flow cytometry |
| Selection guide | Cytokine bioassays guide | cytokines, flow cytometry, immunophenotyping, intracellular staining |
| Selection guide | Intracellular staining guide (for mouse & human cytokines and non-cytokine proteins) | cytokines, flow cytometry, intracellular staining |
| Selection guide | Intracellular staining buffer selection guide | flow cytometry, immunophenotyping, intracellular staining |
| Selection guide | Phospho flow cytometry antibody clone buffer selection guide | flow cytometry, immunophenotyping, intracellular staining, phosphoflow cytometry |
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.