Cell cycle analysis is a very common assay in flow cytometry, and understanding the data is critical to understanding the physiological responses of cells. In this section you will find educational resources to help you with flow cytometric cell cycle analysis experiments including articles, webinars, scientific posters, and more.
Cell cycle features
On demand webinar
DNA content cell cycle analysis using flow cytometry
In this on-demand webinar, find out how careful acquisition and methodical preparation contribute to accurate and consistent DNA analysis.
Scientific poster
Synergistic effect of curcumin in human cell models for chronic lymphocytic leukaemia (PDF)
This scientific poster presentation from ISAC 2018 demonstrates the impact of curcumin (a compound that has been extensively studied in cancer for its proapoptotic, immunomodulatory and antiproliferative properties) on different cell functions including cell cycle and cell proliferation.
Cell cycle learning resources
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| Type | Title | Categories |
|---|---|---|
| BioProbes article (2018) | A comprehensive resource for state-of-the-art flow cytometry methods. Guidelines for the use of flow cytometry and cell sorting in immunological studies | antibodies and proteins, cell counting, cell cycle, cell proliferation, flow cytometer calibration, flow cytometry, flow cytometry compensation, flow cytometry sample preparation, immunology, immunophenotyping, viability |
| BioProbes article (2014) | Multiplex your cell proliferation assay with GFP, RFP, and R-PE probes—Click-iT Plus EdU Proliferation Kits for imaging and flow cytometry | cell proliferation, Click-iT, cell proliferation, multicolor flow cytometry |
| BioProbes article (2013) | Checking vital signs: Don't let dead cells mislead you—Molecular Probes cell health assays for flow cytometry | apoptosis, caspase substrate, cell cycle, cell proliferation, cell tracking, Click-iT, flow cytometry, fluorescence, immunophenotyping, oxidative stress, viability, |
| BioProbes article (2011) | Click-iT EdU Flow Cytometry Kits— Simplifying cell proliferation analysis | cell proliferation, Click-iT, flow cytometry, fluorescence |
| BioProbes article (2009) | A growing list of tools for the violet laser | Alexa Fluor, apoptosis, cell cycle, Click-iT, flow cytometry, fluorescent dyes, immunophenotyping, nanocrystals, Qdot, viability |
| BioProbes article (2009) | Near-infrared dye for live cell cycle analysis | cell cycle, flow cytometry, fluorescent dyes, live-cell imaging, nucleic acid detection |
| BioProbes article (2007) | Improved results are just a click away | Alexa Fluor, cell proliferation, Click-iT, flow cytometry, fluorescent dyes, fluorescence microscopy/fluorescence imaging, high content analysis, in vivo imaging, multicolor flow cytometry, nucleic acid quantitation, nucleosides |
| BioProbes article (2007) | New applications for Vybrant DyeCycle stains | Alexa Fluor, cell cycle, cell isolation, drug discovery, flow cytometry, fluorescent dyes, high content analysis, live-cell imaging, multicolor flow cytometry, stem cell research |
| Scientific poster (2018) | Synergistic effect of curcumin in combination with proapoptotic drugs in human cell models for chronic (CLL) lymphocytic leukaemia | Attune/Attune NxT Flow Cytometer, cell cycle, cancer, drug development, drug research, flow cytometry |
| Scientific poster (2018) | Differently cell doublet profiles: comparison of light scattering with fluorescence measurement techniques | Attune/Attune NxT Flow Cytometer, cell counting, cell cycle, cell viability, flow cytometry, single cell discrimination |
| Scientific Poster (2016) | Cell mediated cytotoxicity in “untouched” whole blood | Attune NxT, cell concentration, cell cycle, flow cytometry assays, viability, whole blood |
| Scientific Poster (2015) | A no lyse, no-wash approach to characterizing phagocyte phenotype and function in whole human blood on the Attune NxT Flow Cytometer | Attune NxT, cell cycle, flow cytometer, flow cytometry assays, immunophenotyping, no lyse, phagocytosis, whole blood |
| Scientific poster (2014) | Copper-safe Click-iT Plus EdU proliferation assay: Improved compatibility with simultaneous phycobiliprotein and fluorescent protein detection | cell proliferation, Click-iT, flow cytometry, immunophenotyping |
| Scientific Poster (2014) | Click-iT EdU Plus for flow cytometry: enhanced compatibility for multiplexing | Attune NxT, cell proliferation, Click-iT, multicolor flow cytometry |
| Scientific Poster (2013) | Cells coming to life: Tools for visualizing immune response | endocytosis/phagocytosis, flow cytometry, fluorescence microscopy/fluorescence imaging, live-cell imaging, pH detection, T cell stimulation |
| Scientific poster (2013) | Improved click chemistry demonstrating EdU cell proliferation with GFP-expressing cells and R-PE-based immunophenotyping | Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometry assays, fluorescent dyes, fluorescent proteins, immunophenotyping |
| Scientific poster (2010) | Analysis of proliferation dynamics in primary and immortalized human cells | Alexa Fluor, cancer, cell proliferation, CellTrace, circulatory system, Click-iT, flow cytometry, fluorescent dyes, fluorescent proteins, nucleic acid quantitation |
| Scientific poster (2010) | Dual pulse labeling using EdU and BrdU to monitor alterations in cellular proliferation by fluorescence microscopy and flow cytometry | Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, drug discovery, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Scientific poster (2010) | Non-cytotoxic near-IR DNA stain for cell cycle analysis in living cells | cell cycle, flow cytometry, fluorescent dyes, fluorescent proteins, live-cell imaging, nucleic acid labeling, viability |
| Scientific poster (2010) | Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry and imaging | Alexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF), immunophenotyping, stem cell research, violet laser-excited reagents |
| Scientific poster (2009) | Dual pulse labeling of S-phase population using click chemistry to measure changes in cell proliferation | Alexa Fluor, cancer, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescent dyes |
| Scientific poster (2009) | Dual pulse labeling using a new thymidine analog 5-ethynyl-2’-deoxyuridine (EdU) to detect alterations in S-phase progression by fluorescence microscopy and flow cytometry | Alexa Fluor, cell cycle, cell proliferation, cells, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Scientific poster (2009) | Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry | Alexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescent dyes, fluorescent proteins, immunophenotyping, nucleic acid quantitation, stem cell research, violet laser-excited reagents |
| Scientific poster (2008) | Characterization of DNA content, cyclin B1 and phosphorylated histone H3 with direct S-phase using EDU incorporation in multiparameter testing of cell lines with cell cycle blocking agents | Alexa Fluor, antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Scientific poster (2008) | Detection of DNA synthesis by automated microscopy and image analysis: Comparison of BRDU method and a new click chemistry-based EDU method | Alexa Fluor, antibodies, ArrayScan, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescent dyes, microplate reader, nucleic acid labeling |
| Scientific poster (2008) | Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry | Alexa Fluor, antibodies, ArrayScan, cell cycle, cells, Click-iT, flow cytometry, fluorescent dyes, microplate reader, nucleic acid labeling, nucleic acid quantitation |
| Scientific poster (2007) | Evaluation of click chemistry-based alternative to BrdU antibody labeling in tissue and cultured cells using fluorescence microscopy and flow cytometry | antibodies, ArrayScan, cell cycle, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, microplate reader, nucleic acid labeling |
| Technology video | Click-iT Plus EdU Proliferation Assay Measuring DNA synthesis is the most precise way to detect changes in cell proliferation. Image-based proliferation assays generate spatial and temporal results that can not be detected with other methods. This video describes and compares two of the most referenced image-based proliferation technologies. | cell proliferation, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging |
| Webinar | DNA content cell cycle analysis using flow cytometry Find out how careful acquisition and methodical preparation contribute to accurate and consistent DNA analysis. In this webinar we’ll discuss: an overview of the methods and materials for using flow cytometry to determine cell cycle by measuring DNA content, selection of DNA dyes for live cell and fixed cell analysis and tips and tricks for consistent results. | cell counting, cell cycle, cell proliferation, flow cytometry |