Antibody conjugates offering superior optical performance
Invitrogen eBioscience eFluor organic dyes were developed specifically for labeling and detection of biomolecules. Each eFluor dye is engineered for superior optical performance in a variety of applications.
eFluor organic dyes are traditional fluorescent dyes for use in multiple applications, including multicolor flow cytometry and immunofluorescence microscopy. To make fluorophore selection easier, all eFluor organic dyes are named after their emission wavelength and they are fully compatible with most conventional dyes.
| Dye | eFluor 450 | eFluor 506 |
|---|---|---|
| Fluorophore type | Organic dye | Organic dye |
| Ex (nm) | 401 | 419 |
| Em (nm) | 421 | 506 |
| Application | Flow cytometry | Flow cytometry |
| Conjugates available | eFluor 450 primary antibody conjugates eFluor 450 secondary antibody conjugates | |
| Recommended bandpass filter | 450/50 | 510/50 |
| Uses | Comparable to Invitrogen Pacific Blue dye | Use in place of AmCyan or Horizon V500 dye |
Learn more about flow antibodies for the violet laser
| Dye | eFluor 520 | eFluor 570 | PE-eFluor 610 | PerCP-eFluor 710 | |
|---|---|---|---|---|---|
Fluorophore type | Organic dye | Organic dye | Organic dye (tandem), resonance energy transfer from PE molecule to eFluor 610 dye | Organic dye (tandem), resonance energy transfer from PerCP molecule to eFluor 710 dye | |
Ex (nm) | 475 | 546 | 496, 565 | 488 | |
Em (nm) | 535 | 585 | 607 | 710 | |
Application | ICC, IHC | ICC, IHC | FC | FC | |
Conjugates available | |||||
Recommended bandpass filter | 535/45 | 585/40 | 610/20 | 695/40 | |
Uses | Alternative to Invitrogen Alexa Fluor 488 dye | Alternative to Invitrogen Alexa Fluor 555 dye | Alternative to Invitrogen PE-Texas Red dye (Note: PE-eFluor 610 is brighter and requires reduced compensation from far-red channels compared to PE-Texas Red dye) | Alternative to PE-Cyanine 5.5 dye or PerCP-Cyanine 5.5 | |
Learn more about flow antibodies for the blue laser
| Dye | eFluor 570 | eFluor 615 |
|---|---|---|
| Fluorophore type | Organic dye | Organic dye |
| Ex (nm) | 546 | 560 |
| Em (nm) | 585 | 645 |
| Application | ICC, IHC | ICC, IHC |
| Conjugates available | ||
| Recommended bandpass filter | 585/40 | 645/75 |
| Uses | Alternative to Invitrogen Alexa Fluor 555 dye | Alternative to Invitrogen Alexa Fluor 647 dye |
Learn more about flow antibodies for the green laser
| Dye | eFluor 660 | APC-eFluor 780 |
|---|---|---|
| Fluorophore type | Organic dye | Organic dye (tandem), resonance energy transfer from APC molecule to eFluor 780 dye |
| Ex (nm) | 633 | 645 |
| Em (nm) | 668 | 767 |
| Application | Flow cytometry, ICC, IHC | Flow cytometry |
| Conjugates available | ||
| Recommended bandpass filter | 660/20 | 780/60 |
| Uses | Alternative to APC or Invitrogen Alexa Fluor 647 dye | Comparable to APC-Cyanine 7 and Invitrogen APC-Alexa Fluor 750 dye |
Learn more about flow antibodies for the red laser
Resources
Custom Services
Contact our eBioscience custom services group for antibody conjugation, antibody cocktails, immunoassay development, bulk reagents, and testing services.
Support
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.